scholarly journals The Antibacterial Properties of Bayur Tissues’ Extract (Pterospermum subpeltatum C.B. Rob)

2014 ◽  
Vol 69 (5) ◽  
Author(s):  
Pince Salempa ◽  
Alfian Noor ◽  
Tjodi Harlim ◽  
Nunuk Hariani ◽  
Muharram Muharram ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Traditional Medicine ◽  
Secondary Metabolite ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Shigella Boydii ◽  
And Staphylococcus Aureus ◽  
Antibacterial Potential

Pterospermum is of plant genus in the Sterculiaceae family, which has never been studied for its secondary metabolite compound. The others species from this genus have long been used in generations of traditional medicine. For example, P. Javanicum has been used to treat dysentery, toothaches and ulcers. From the antibacterial testing carried out on some tissues of Pterospermum subpeltatum by GNA diffusion method,  this plant showed antibacterial potential, especially toward Shigella boydii and Staphylococcus aureus.

scholarly journals Antibacterial Activity of Clay Soils against Food-Borne Salmonella Typhimurium and Staphylococcus aureus

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 170
Author(s):  
Nur Naqiyah Azmi ◽  
Nor Ainy Mahyudin ◽  
Wan Hasyera Wan Omar ◽  
Nor-Khaizura Mahmud Ab Rashid ◽  
Che Fauziah Ishak ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Salmonella Typhimurium ◽  
Metal Ion ◽  
Antimicrobial Agents ◽  
Antibacterial Properties ◽  
Xrd Analysis ◽  
Diffusion Method ◽  
Food Borne ◽  
And Staphylococcus Aureus

Natural clays have recently been proven to possess antibacterial properties. Effective natural antimicrobial agents are needed to combat bacterial contamination on food contact surfaces, which are increasingly more prevalent in the food chain. This study sought to determine the antibacterial activity of clays against the food-borne pathogens Salmonella Typhimurium ATCC 14028 and Staphylococcus aureus ATCC 13565. Soils were processed to yield leachates and suspensions from untreated and treated clays. Soil particle size, pH, cation-exchange capacity, metal composition and mineralogy were characterized. Antibacterial screening was performed on six Malaysian soils via the disc diffusion method. In addition, a time-kill assay was conducted on selected antibacterial clays after 6 h of exposure. The screening revealed that Munchong and Carey clays significantly inhibit Salmonella Typhimurium (11.00 ± 0.71 mm) and S. aureus (7.63 ± 0.48 mm), respectively. Treated Carey clay leachate and suspension completely kill Salmonella Typhimurium, while S. aureus viability is reduced (2 to 3 log10). The untreated Carey and all Munchong clays proved ineffective as antibacterials. XRD analysis confirmed the presence of pyrite and magnetite. Treated Carey clays had a higher soluble metal content compared to Munchong; namely Al (92.63 ± 2.18 mg/L), Fe (65.69 ± 3.09 mg/L) and Mg (88.48 ± 2.29 mg/L). Our results suggest that metal ion toxicity is responsible for the antibacterial activity of these clays.

Characterization and Antibacterial Potential of Tithonia diversifolia Extract and Its Iron II Nanoparticles

2020 ◽  
pp. 101-107
Author(s):  
Hellen Chebet ◽  
Johnson Kinyua ◽  
Patrick Kareru ◽  
Njiema Gitaari
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Tithonia Diversifolia ◽  
Gram Positive Bacteria ◽  
And Staphylococcus Aureus ◽  
Positive Controls ◽  
Antibacterial Potential

Several plants worldwide have exhibited potentiality in human pathogen eradication. The aim of this work was to characterize the compounds and determine antibacterial efficiency of Tithonia diversifolia essential oils and its iron II nanoparticles. The experimental test was done against gram positive bacteria (Bacillus subtilis, Staphylococcus aureus) and gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa). Characterization of the compounds was done using UV-VIS and FTIR to determine the functional groups that are present. Antibacterial activity was done using disc diffusion method by Beer Lambert. The results obtained indicated that the crude extract at 0.1% v/v concentration was effective against Pseudomonas aeruginosa and Staphylococcus aureus only, while the complexed sample was significantly effective against all microbes under test at the corresponding concentration. The outcome showed comparable results to the positive controls used and therefore, the plant extract was effective against human disease causing microbes and is recommendable for use against them.

Antioxidant and Antimicrobial Activities of the Fresh aerial parts of Ammi visnaga L. from Algeria

2016 ◽  
Vol 6 (2) ◽  
pp. 70-76
Author(s):  
Soumia Keddari ◽  
Narimen Benaoum ◽  
Yasmina Mokhtaria Boufadi ◽  
Mansouria Belhocine ◽  
Ali Riazi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Ammi Visnaga ◽  
Wide Range

Medicinal plants have been used for countries as cures for human diseases because they contain components of therapeutic value. Among these medi-cinal plants, Ammi visnage which have an immense reservoir of potential compounds attributed to the secondary metabolites which have the advan-tage of being of great diversity of chemical structure and have a very wide range of biological activities. The objectives of the present work were to stu-dy the antioxidant and antimicrobial activity of phenolic compounds ex-tracted from A. visnaga L. Its extraction is performed by two methods, etha-nol extraction and water extraction. The results showed that A. visnaga L.. ethanolic extract contains a mixture of phytochemical classes as polyphenol, flavonoids and revealed that this plant has high antioxidant activity (IC50 0.069 mg/ml). Regarding the antimicrobial activity results expressed by the diameter of the inhibition zones by diffusion method AWDT, the most signifi-cant inhibition was observed against to Staphylococcus aureus (12 mm) to the ethanol extract at concentration of 100mg / ml. Thus the aqueous ex-tract had a significant inhibitory activity against on the strains Staphylococ-cus aureus (8 mm), E. coli ATCC 10536 (8 mm) to a concentration of 100 mg / ml. The results for the antibacterial properties have shown that Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and M. luteus.) were more sensitive than gram-negative (Pseudomonas aeruginosa, E. coli ATCC 10536) against from the action of phenolic compounds of the Ammi visnaga ethanolic extract.

scholarly journals Hop Extract Acts as an Antioxidant with Antimicrobial Effects against Propionibacterium Acnes and Staphylococcus Aureus

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 223 ◽  
Author(s):  
Natalja Weber ◽  
Klaus Biehler ◽  
Kay Schwabe ◽  
Birgit Haarhaus ◽  
Karl-W. Quirin ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Propionibacterium Acnes ◽  
Inhibition Zone ◽  
Positive Control ◽  
Antioxidative Effect ◽  
Minimal Inhibitory Concentrations ◽  
Anti Inflammatory ◽  
And Staphylococcus Aureus ◽  
And Oxidative Stress ◽  
Antibacterial Potential

Acne is associated with hyperkeratosis, elevated levels of skin sebum and growth of Propionibacterium acnes (P. acnes) and Staphylococcus aureus (S. aureus). Furthermore, P. acnes promotes inflammation by inducing IL-6 production and oxidative stress. The aim of this study was to assess the antioxidant, anti-inflammatory and antibacterial potential of a hop-CO2-extract with 50% humulone and lupulone. The susceptibility of P. acnes and S. aureus to the hop extract was tested by using the broth microdilution technique. The minimal inhibitory concentrations (MIC) for P. acnes and S. aureus were 3.1 and 9.4 µg/mL, respectively. In addition, the hop extract showed an antioxidative effect with a half maximal inhibitory concentration (IC50) of 29.43 µg/mL as well as additional anti-inflammatory effects by reducing the IL-6 expression (IC50: 0.8 µg/mL). In addition, a gel formulation with 0.3% hop extract (w/w) had antibacterial activity against P. acnes and S. aureus (inhibition zone value: 5.5 mm and 3 mm, respectively) which was significantly superior to the placebo gel. The positive control (a gel with the antibiotic clindamycin) showed an inhibition zone of 9 mm. Due to its antioxidant, anti-inflammatory and antibacterial effects hop extract might be a treatment option for acne-prone skin.

scholarly journals AKTIVITAS ANTIMIKROBA EKSTRAK DAUN JAMBU BIJI DARI BEBERAPA KULTIVAR TERHADAP Staphylococcus aureus ATCC 25923 DENGAN HOLE-PLATE DIFFUSION METHOD

2003 ◽  
Vol 9 (1) ◽  
pp. 49-51
Author(s):  
Farida Lanawati Darsono ◽  
Stephanie Devi Artemisia
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Traditional Medicine ◽  
Tween 80 ◽  
Antibacterial Activities ◽  
Psidium Guajava ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Gram Positive Bacteria ◽  
Activity Test

A study has been performed on the antimicrobial activities of jambu biji (Psidium guajava Linn) leaves from several cultivars (red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that the leaves of jambu biji are frequently used in traditional medicine as a remedy against diarrhoea. The hole-plate diffusion method was used for conducting the antimicrobial activity test with antibiotics (Ampicilline trihidrat) as reference standards. The extracts of jambu biji for each cultivar were obtained by reflux with ethanol 96 percent. The concentrations of the extracts applied to the holes were 10 percent, 20 percent, and 30 percent w/v, the extracts were reconstituted with tween 80 and ethanol 96 percent. Based on the result of the study, it can be concluded that the extract of jambu biji from each cultivar with the concentration of 10 percent, 20 percent, and 30 percent w/v showed antibacterial activities against Staphylococcus aureus ATCC 25923. The result obtained statictically evaluated using Anava Factorial 3x3 and furthery tested for significancy (a = 0.05). Based on the results of study, it can be concluded that the extract of jambu biji leaves from red cultivar, white cultivar and yellow cultivar showed antibacterial activities against Staphylococcus aureus ATCC 25923.

scholarly journals Preliminary Screening of Bacteria Isolated from Insects Living in Poultry for Antibiotic Production

2020 ◽  
pp. 49-57
Author(s):  
Ifeoma Geraldine Okudo ◽  
Chinelo Ursula Umedum ◽  
Stephen Nnaemeka Ezekwueche ◽  
Chibuzo Christian Uba
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Antibiotic Production ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
Preliminary Screening ◽  
Agar Plug ◽  
And Staphylococcus Aureus

Aim: This present study was conducted to isolate antibiotic producing bacteria from insects living in poultry. Place and Duration of Study: Insects living in poultry were collected from poultry farms within Onitsha metropolis in Anambra State between April 2018 and September 2018. Methodology: The gut of one hundred insects; (Musca domestica and Alphitobius diaperinus) were analyzed. The insects were dissected and emulsified in 10ml of peptone water. The dilutions were cultured on Nutrient agar and Blood agar  for 24 h. The bacterial isolates were characterized using  molecular identification. The DNA was extracted from the identified isolates and analyzed by 16S rRNA. In preliminary screening, the isolates were inoculated into Muller Hinton agar using agar plug. The promising isolate showing antagonism was subjected to submerged fermentation method and the secondary metabolites were extracted. Screening of the secondary metabolites extract was done using agar well diffusion. The minimum inhibitory concentration (MIC) of the secondary metabolite was determined using broth dilution method at different concentrations. The inhibitory activity of the organism was checked against four bacteria namely; Bacillus subtilis, Salmonella serovar typhi, Escherichia coli and Staphylococcus aureus. Results: The sequence analysis revealed the strains to be Lysinibacillus macroides, Paealcaligenes hermetiae, Bordetella flabilis, Bacillus aerophilus, Klebsiella variicola. Lysinibacillus macroides showed antagonism against the test bacteria during the preliminary test. After fermentation, the secondary metabolite extracts from Lysinibacillus macroides exhibited antibacterial activities against Salmonella Serovar Typhi, Staphyloccus aureus and Bacillus subtilis at different concentrations. Conclusion: The extracts from bacterial isolate; Lysinibacillus macroides exhibited antibacterial activities against Bacillus subtilis, Salmonella serovar typhi and Staphylococcus aureus. The extracts may serve as a new drug molecule produced from natural source when purified.

scholarly journals Antibacterial activity of different extract of medicinal plant Antirrihnum Orontium.

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Himayat Ullah ◽  
Shehzad Saleem ◽  
Wisal Ahmad ◽  
Muhammad Hanif ◽  
Zahid Ali ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Water Extract ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Water Extracts ◽  
The Family ◽  
Quaternary Base ◽  
And Staphylococcus Aureus

Antirrhinum Orontium is belonging to the family Plantaginacea commonly known as lesser snapdragon which is a flowering plant belong to genus Antirrhinum. It was traditionally used in treatment of Tumor, Eye inflammation and Ulcer. Antirrhinum Orontium is contain mostly tertiary alkaloids bases one of them is identified as 4-methyl-2, 6-naphthyridine and Choline is identified as quaternary base. The recent study is to investigate the antibacterial activity of Ethyl acetate, Dichloromethane, Hexane and Water extracts of Antirrhinum Orontium, against the pathogen like Escherichia coli and Staphylococcus aureus. Both the Soxhlet and Maceration water extract were found most active from the other extract against Escherichia coli and Staphylococcus aureus by using agar well diffusion method. The measured zone of inhibition for Soxhlet water extract is 21±0.05mm and Maceration water extract is 20±0.05mm against gram positive Staphylococcus aureus, while for gram negative Escherichia coli 23±0.05mm is measured for Soxlet and 21±0.05mm measured for Maceration water extracts

scholarly journals Antibacterial Activity of Biologically Synthesized Silver and Zinc Nanoparticles Using Allcemilla vulgaris (Layd’s Mantle) Leaf Extract

2019 ◽  
pp. 1-11
Author(s):  
Dilek Demirezen-Yilmaz ◽  
Fatih Doğan Koca ◽  
Nurhan Ertaş-Onmaz
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Escherichia Coli O157 ◽  
X Ray Diffraction ◽  
Zinc Nanoparticles ◽  
X Ray ◽  
Vis Spectroscopy ◽  
And Staphylococcus Aureus ◽  
Antibacterial Potential

In this study, the powder of Allcemilla vulgaris was used in the sythesis of silver and zinc nanoparticle. Biologically synthesized nanoparticles were characterized using Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), UV-Vis spectroscopy, X-ray diffraction (XRD) and Zeta potential and then evaluated for antibacterial potential using micro dilution broth method. The minimum inhibitory concentration values of AgNP were 4.25 µg/mL and 6.64 µg/ mL for Escherichia coli O157:H7 and Staphylococcus aureus, respectively. Similarly, the MIC values of ZnNP were 3.32 µg/mL and 6.25 µg/mL, respectively for Escherichia coli O157:H7 and Staphylococcus aureus.

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