Tracing Clostridium perfringens strains along the chicken production chain from farm to slaughter by multilocus sequence typing

2021 ◽  
Author(s):  
Wenping Xu ◽  
Hairong Wang ◽  
Suo Chen ◽  
Yong Chen ◽  
Lixue Liu ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Multilocus Sequence Typing ◽  
Production Chain

Multilocus sequence typing of Clostridium perfringens strains from neonatal calves, dairy workers and associated environment in India

Anaerobe ◽  
2020 ◽  
Vol 63 ◽  
pp. 102212
Author(s):  
Asha Kumari Verma ◽  
Mostafa Y. Abdel-Glil ◽  
Angappan Madesh ◽  
Shailendri Gupta ◽  
Athira Cheruplackal Karunakaran ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Multilocus Sequence Typing ◽  
Dairy Workers ◽  
Neonatal Calves

scholarly journals Multilocus Sequence Typing Analysis of Clostridium perfringens Isolates from Necrotic Enteritis Outbreaks in Broiler Chicken Populations

2008 ◽  
Vol 46 (12) ◽  
pp. 3957-3964 ◽  
Author(s):  
G. Chalmers ◽  
H. L. Bruce ◽  
D. B. Hunter ◽  
V. R. Parreira ◽  
R. R. Kulkarni ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Multilocus Sequence Typing ◽  
Broiler Chicken ◽  
Necrotic Enteritis

scholarly journals The Circulation of Type F Clostridium perfringens among Humans, Sewage, and Ruditapes philippinarum (Asari Clams)

Pathogens ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 669 ◽  
Author(s):  
Keita Yanagimoto ◽  
Kosei Uematsu ◽  
Takaya Yamagami ◽  
Eiji Haramoto
Keyword(s):  
Clostridium Perfringens ◽  
Causative Agent ◽  
Multilocus Sequence Typing ◽  
Water Environment ◽  
Ruditapes Philippinarum ◽  
Foodborne Diseases ◽  
Sewage Pollution

Clostridium perfringens is an important pathogen that is responsible for gastroenteritis; the causative agent for the symptoms is C. perfringens enterotoxin (CPE), which is mainly produced by type F C. perfringens. Since shellfishes may gather C. perfringens in the water environment, this study estimated the potential circulation of type F C. perfringens among humans, sewage, and Ruditapes philippinarum (asari clams) as a result of sewage pollution. A comparison of the characteristics among the isolates from 86 sewage influents, 36 effluents, 76 asari clams, and 37 humans was conducted. Serotyping, cpe genotyping, and toxin genotyping showed that C. perfringens with a plasmid IS1151 sequence downstream of cpe was predominant among sewage influents, effluents, humans, and asari clams. Multilocus sequence typing suggested that some isolates from a human, sewage influents, effluents, and asari clams were linked to each other. These results demonstrated that asari clams are the necessary infection sources of C. perfringens responsible for carriers and foodborne diseases, and that these pathogens from humans infected by asari clams can pollute the water environment. It is useful to assess bacteria such as C. perfringens isolates from sewage to estimate the trend of those from the community.

scholarly journals Prevalence and multilocus sequence typing of Clostridium perfringens isolated from 4 duck farms in Shandong province, China

2020 ◽  
Vol 99 (10) ◽  
pp. 5105-5117 ◽  
Author(s):  
Li Xiu ◽  
Yu Liu ◽  
Wei Wu ◽  
Suo Chen ◽  
Zhaobing Zhong ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Multilocus Sequence Typing ◽  
Shandong Province

scholarly journals A Wide Variety of Clostridium perfringens Type A Food-Borne Isolates That Carry a ChromosomalcpeGene Belong to One Multilocus Sequence Typing Cluster

2012 ◽  
Vol 78 (19) ◽  
pp. 7060-7068 ◽  
Author(s):  
Yinghua Xiao ◽  
Arjen Wagendorp ◽  
Roy Moezelaar ◽  
Tjakko Abee ◽  
Marjon H. J. Wells-Bennik
Keyword(s):  
Clostridium Perfringens ◽  
Multilocus Sequence Typing ◽  
Food Poisoning ◽  
Distinct Group ◽  
Nationwide Survey ◽  
Type A ◽  
Evolutionary Significance ◽  
Pcr Analysis ◽  
Content Type ◽  
Food Borne

ABSTRACTOf 98 suspected food-borneClostridium perfringensisolates obtained from a nationwide survey by the Food and Consumer Product Safety Authority in The Netherlands, 59 strains were identified asC. perfringenstype A. Using PCR-based techniques, thecpegene encoding enterotoxin was detected in eight isolates, showing a chromosomal location for seven isolates and a plasmid location for one isolate. Further characterization of these strains by using (GTG)5fingerprint repetitive sequence-based PCR analysis distinguishedC. perfringensfrom other sulfite-reducing clostridia but did not allow for differentiation between various types ofC. perfringensstrains. To characterize theC. perfringensstrains further, multilocus sequence typing (MLST) analysis was performed on eight housekeeping genes of both enterotoxic and non-cpeisolates, and the data were combined with a previous global survey covering strains associated with food poisoning, gas gangrene, and isolates from food or healthy individuals. This revealed that the chromosomalcpestrains (food strains and isolates from food poisoning cases) belong to a distinct cluster that is significantly distant from all the othercpeplasmid-carrying andcpe-negative strains. These results suggest that different groups ofC. perfringenshave undergone niche specialization and that a distinct group of food isolates has specific core genome sequences. Such findings have epidemiological and evolutionary significance. Better understanding of the origin and reservoir of enterotoxicC. perfringensmay allow for improved control of this organism in foods.

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