Pancreas preservation with amphotericin B deteriorates islet yield for porcine islet isolation

2021 ◽  
Author(s):  
Sayaka Sawada ◽  
Chika Miyagi‐Shiohira ◽  
Kazuho Kuwae ◽  
Yoshihito Tamaki ◽  
Kai Nishime ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Islet Isolation ◽  
Porcine Islet ◽  
Pancreas Preservation

scholarly journals Excellent Islet Yields after 18-h Porcine Pancreas Preservation by Ductal Injection, Pancreas Preservation with MK Solution, Bottle Purification, and Islet Purification Using Iodixanol with UW Solution and Iodixanol with MK Solution

2019 ◽  
Vol 8 (10) ◽  
pp. 1561 ◽  
Author(s):  
Kazuho Kuwae ◽  
Chika Miyagi-Shiohira ◽  
Eri Hamada ◽  
Yoshihito Tamaki ◽  
Kai Nishime ◽  
...  
Keyword(s):  
Stimulation Index ◽  
Islet Isolation ◽  
Preservation Solution ◽  
Porcine Pancreas ◽  
Porcine Islet ◽  
University Of Wisconsin ◽  
Pancreas Preservation ◽  
Purification Methods ◽  
Pancreas Weight ◽  
Wisconsin Solution

Successful islet isolation is the key to successful islet transplantation. Our group recently modified the islet isolation protocol to include pancreatic ductal injection of the preservation solution, pancreas storage in modified extracellular-type trehalose-containing Kyoto (MK) solution, and use of an iodixanol-based purification solution and bottle purification. In this study, we applied these methods to porcine islet isolation after 18-h pancreas preservation and compared two solutions with different compositions in bottle purification. Islet yield before purification was 651,661 ± 157,719 islet equivalents (IE) and 5576 ± 1538 IE/g pancreas weight. An IU solution was made by adding iodixanol to University of Wisconsin solution and an IK solution was made by adding iodixanol to MK solution. The efficacy of the two solutions for islet isolation was compared. There were no significant differences between the two purification methods with regard to islet yield, survival rate, purity, score, or stimulation index. These results indicate that our isolation protocol produces efficient islet yields from prolonged cold-stored pancreas and that IU and IK solutions are equally useful for islet purification.

Pancreas Preservation in Modified Histidine-lactobionate Solution Is Superior to That in University of Wisconsin Solution for Porcine Islet Isolation

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Mayuko Sakai-Yonaha ◽  
Chika Miyagi-Shiohira ◽  
Kazuho Kuwae ◽  
Yoshihito Tamaki ◽  
Kai Nishime ◽  
...  
Keyword(s):  
Islet Isolation ◽  
University Of Wisconsin Solution ◽  
Porcine Islet ◽  
University Of Wisconsin ◽  
Pancreas Preservation ◽  
Wisconsin Solution

Pancreas preservation in extracellular‐type p38 inhibitor‐containing solution improves islet yield for porcine islet isolation

2020 ◽  
Author(s):  
Tasuku Yonaha ◽  
Chika Miyagi‐Shiohira ◽  
Kazuho Kuwae ◽  
Yoshihito Tamaki ◽  
Kai Nishime ◽  
...  
Keyword(s):  
Islet Isolation ◽  
P38 Inhibitor ◽  
Porcine Islet ◽  
Pancreas Preservation

scholarly journals Improvement of Porcine Islet Isolation by Inhibition of Trypsin Activity during Pancreas Preservation and Digestion Using α1-Antitrypsin

2012 ◽  
Vol 21 (2-3) ◽  
pp. 465-471 ◽  
Author(s):  
Masayuki Shimoda ◽  
Hirofumi Noguchi ◽  
Yasutaka Fujita ◽  
Morihito Takita ◽  
Tetsuya Ikemoto ◽  
...  
Keyword(s):  
Islet Isolation ◽  
Trypsin Activity ◽  
Porcine Islet ◽  
Pancreas Preservation

Prevention of oxidative stress in porcine islet isolation

2010 ◽  
Vol 13 (1) ◽  
pp. 38-47 ◽  
Author(s):  
Philipp Stiegler ◽  
Vanessa Stadlbauer ◽  
Florian Hackl ◽  
Silvia Schaffellner ◽  
Florian Iberer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Islet Isolation ◽  
Porcine Islet

scholarly journals A Preliminary Study of the Activation of Endogenous Pancreatic Exocrine Enzymes during Automated Porcine Islet Isolation

1999 ◽  
Vol 8 (3) ◽  
pp. 265-276 ◽  
Author(s):  
S. A. White ◽  
H. Djaballah ◽  
D. P. Hughes ◽  
D. L. Roberts ◽  
H. H. Contractor ◽  
...  
Keyword(s):  
Islet Isolation ◽  
Porcine Islet ◽  
Pancreatic Exocrine ◽  
Preliminary Study

scholarly journals A Novel Preservation Solution Containing a JNK Inhibitory Peptide Efficiently Improves Islet Yield for Porcine Islet Isolation

2019 ◽  
Vol 103 (2) ◽  
pp. 344-352 ◽  
Author(s):  
Hirofumi Noguchi ◽  
Chika Miyagi-Shiohira ◽  
Yoshiki Nakashima ◽  
Nana Ebi ◽  
Eri Hamada ◽  
...  
Keyword(s):  
Islet Isolation ◽  
Inhibitory Peptide ◽  
Preservation Solution ◽  
Porcine Islet

Excellence of the two-layer method (University of Wisconsin solution/perfluorochemical) in pancreas preservation before islet isolation

Surgery ◽  
1997 ◽  
Vol 122 (2) ◽  
pp. 435-442 ◽  
Author(s):  
Yasuki Tanioka ◽  
David E.R Sutherland ◽  
Yoshikazu Kuroda ◽  
Thomas R Gilmore ◽  
Tor C Asaheim ◽  
...  
Keyword(s):  
Islet Isolation ◽  
University Of Wisconsin Solution ◽  
University Of Wisconsin ◽  
Layer Method ◽  
Pancreas Preservation ◽  
Wisconsin Solution

THE INFLUENCE OF PANCREAS PRESERVATION ON HUMAN ISLET ISOLATION OUTCOMES: IMPACT OF THE TWO-LAYER METHOD

2004 ◽  
Vol 78 ◽  
pp. 352
Author(s):  
T Tsujimura ◽  
Y Kuroda ◽  
J Avila ◽  
T Kin ◽  
A M.J. Shapiro ◽  
...  
Keyword(s):  
Human Islet ◽  
Islet Isolation ◽  
Layer Method ◽  
Pancreas Preservation
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