scholarly journals The role of bacterial extracellular vesicles in chronic wound infections: Current knowledge and future challenges

2021 ◽  
Author(s):  
Helen L. Brown ◽  
Aled Clayton ◽  
Phil Stephens
Keyword(s):  
Extracellular Vesicles ◽  
Chronic Wound ◽  
Current Knowledge ◽  
Wound Infections ◽  
Future Challenges ◽  
Chronic Wound Infections

scholarly journals Biofilm Formation Reducing Properties of Manuka Honey and Propolis in Proteus mirabilis Rods Isolated from Chronic Wounds

2020 ◽  
Vol 8 (11) ◽  
pp. 1823
Author(s):  
Joanna Kwiecińska-Piróg ◽  
Jana Przekwas ◽  
Michał Majkut ◽  
Krzysztof Skowron ◽  
Eugenia Gospodarek-Komkowska
Keyword(s):  
Proteus Mirabilis ◽  
Biofilm Formation ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Ethanol Extract ◽  
Minimal Bactericidal Concentration ◽  
Wound Infections ◽  
Manuka Honey ◽  
The Impact ◽  
Chronic Wound Infections

Chronic wound infections are difficult to manage because of the biofilm formation in the wound environment. New measures for eliminating infections are necessary to increase the chance of wound healing. Apitherapy may be the new solution. The aim of this study was to assess the prevalence of wound infection factors and to examine the impact of Manuka honey and ethanol extract of propolis on biofilm formation of Proteus mirabilis isolated from chronic wound infections. According to the findings, the most frequent factors of infection are Staphylococcus aureus (46.1%), Pseudomonas aeruginosa (35.0%), and Proteus mirabilis (10.6%). Minimal inhibitory concentration and minimal bactericidal concentration values were assigned using the microbroth dilution test according to the Clinical and Laboratory Standards Institute. Biofilm of Proteus mirabilis isolates was formed in 96-well polystyrene plates and treated with Manuka honey (concentrations from 1.88% to 30.0%) and ethanol extract of propolis (1.0% to 40.0%). After 24 h, the biofilm viability was expressed by formazan absorbance (λ = 470 nm). Manuka honey reduced the biofilm viability in all, and ethanol extract of propolis in most, of the concentrations tested. Ethanol extract of propolis at the concentrations of 20.0% and 40.0%, reduced biofilm viability stronger than ethanol itself. With these results comes the conclusion that these substances can reduce biofilm formation.

Treating Chronic Wound Infections with Genetically Modified Free Flaps

2009 ◽  
Vol 123 (4) ◽  
pp. 1157-1168 ◽  
Author(s):  
Shadi Ghali ◽  
Kirit A. Bhatt ◽  
Marlese P. Dempsey ◽  
Deidre M. Jones ◽  
Sunil Singh ◽  
...  
Keyword(s):  
Chronic Wound ◽  
Genetically Modified ◽  
Free Flaps ◽  
Wound Infections ◽  
Chronic Wound Infections

Chronic wound infections: the role ofPseudomonas aeruginosaandStaphylococcus aureus

2015 ◽  
Vol 13 (5) ◽  
pp. 605-613 ◽  
Author(s):  
Raffaele Serra ◽  
Raffaele Grande ◽  
Lucia Butrico ◽  
Alessio Rossi ◽  
Ugo Francesco Settimio ◽  
...  
Keyword(s):  
Chronic Wound ◽  
Wound Infections ◽  
Chronic Wound Infections

scholarly journals Hypertonic glucose inhibits growth and attenuates virulence factors of multidrug-resistant Pseudomonas aeruginosa

2020 ◽  
Author(s):  
Tao Chen ◽  
Ye Xu ◽  
Wenya Xu ◽  
Wenli Liao ◽  
Chunquan Xu ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
Diabetic Foot ◽  
Biofilm Formation ◽  
Chronic Wound ◽  
Multidrug Resistant ◽  
Wound Infections ◽  
Hypertonic Glucose ◽  
Diabetic Foot Infections ◽  
Chronic Wound Infections

Abstract Background: Pseudomonas aeruginosa is the most common Gram-negative pathogen responsible for chronic wound infections, such as diabetic foot infections, and further exacerbates the treatment options and cost of such conditions. Hypertonic glucose, a commonly used prolotherapy solution, can accelerate the proliferation of granulation tissue and improve microcirculation in wounds. However, the action of hypertonic glucose on bacterial pathogens that infect wounds is unclear. In this study, we investigated the inhibitory effects of hypertonic glucose on multidrug-resistant P. aeruginosa strains isolated from diabetic foot infections. Hypertonic glucose represents a novel approach to control chronic wound infections caused by P. aeruginosa. Results: Four multidrug-resistant P. aeruginosa clinical strains isolated from diabetic foot ulcers from a tertiary hospital in China and the reference P. aeruginosa PAO1 strain were studied. Hypertonic glucose significantly inhibited the growth, biofilm formation, and swimming motility of P. aeruginosa clinical strains and PAO1. Furthermore, hypertonic glucose significantly reduced the production of pyocyanin and elastase virulence factors in P. aeruginosa. The expression of major quorum sensing genes (lasI, lasR, rhlI, and rhlR) in P. aeruginosa were all downregulated in response to hypertonic glucose treatment. In a Galleria mellonella larvae infection model, the administration of hypertonic glucose was shown to increase the survival rates of larvae infected by P. aeruginosa strains (3/5).Conclusions: Hypertonic glucose inhibited the growth, biofilm formation, and swimming motility of P. aeruginosa, as well as reduced the production of virulence factors and quorum sensing gene expression. Further studies that investigate hypertonic glucose therapy should be considered in treating chronic wound infections.

scholarly journals Trichophytonas a Rare Cause of Postoperative Wound Infection Resistant to Standard Empiric Antimicrobial Therapy

2018 ◽  
Vol 2018 ◽  
pp. 1-3
Author(s):  
Sheema Gaffar ◽  
John K. Birknes ◽  
Kenji M. Cunnion
Keyword(s):  
Wound Infection ◽  
Tinea Capitis ◽  
Chronic Wound ◽  
Fungal Infections ◽  
Surgical Wound Infection ◽  
Postoperative Wound Infection ◽  
Wound Infections ◽  
Surgical Wound ◽  
Trichophyton Tonsurans ◽  
Chronic Wound Infections

Fungal infections are rare causes of acute surgical wound infections, butCandidais not an infrequent etiology in chronic wound infections.Trichophytonspecies is a common cause of tinea capitis but has not been reported as a cause of neurosurgical wound infection. We report a case ofTrichophyton tonsuranscausing a nonhealing surgical wound infection in a 14-year-old male after hemicraniectomy. His wound infection was notable for production of purulent exudate from the wound and lack of clinical improvement despite empiric treatment with multiple broad-spectrum antibiotics targeting typical bacterial causes of wound infection. Multiple wound cultures consistently grewTrichophytonfungus, and his wound infection clinically improved rapidly after starting terbinafine and discontinuing antibiotics.

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