scholarly journals Endotoxin testing of a wound debridement device containing medicinal Lucilia sericata larvae

2017 ◽  
Vol 25 (3) ◽  
pp. 498-501
Author(s):  
Samantha F. Pickles ◽  
David I. Pritchard
Keyword(s):  
Lucilia Sericata ◽  
Wound Debridement

scholarly journals Gene expression in Lucilia sericata (Diptera: Calliphoridae) larvae exposed to Pseudomonas aeruginosa and Acinetobacter baumanii identifies shared and microbe-specific induction of immune genes

2021 ◽  
Author(s):  
Claire H McKenna ◽  
Danial Asgari ◽  
Tawni C Crippen ◽  
Le Zheng ◽  
Ronald A Sherman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pseudomonas Aeruginosa ◽  
Pathogenic Bacteria ◽  
Molecular Level ◽  
Lucilia Sericata ◽  
Acinetobacter Baumanii ◽  
Wound Debridement ◽  
Immune Genes ◽  
Specific Induction ◽  
Deficiency Gene

Antibiotic resistance is a continuing challenge in medicine. There are various strategies for expanding antibiotic therapeutic repertoires, including the use of blow flies. Their larvae exhibit strong antibiotic and antibiofilm properties that alter microbiome communities. One species, Lucilia sericata, is used to treat problematic wounds due to its debridement capabilities and its excretions and secretions that kill some pathogenic bacteria. There is much to be learned about how L. sericata interacts with microbiomes at the molecular level. To address this deficiency, gene expression was assessed after feeding exposure (1 hour or 4 hours) to two clinically problematic pathogens: Pseudomonas aeruginosa and Acinetobacter baumanii. The results identified immunity related genes that were differentially expressed when exposed to these pathogens, as well as non-immune genes possibly involved in gut responses to bacterial infection. There was a greater response to P. aeruginosa that increased over time, while few genes responded to A. baumanii exposure and expression was not time-dependent. The response to feeding on pathogens indicates a few common responses and features distinct to each pathogen, which is useful in improving wound debridement therapy and helps develop biomimetic alternatives.

Wound debridement potential of glycosidases of the wound-healing maggot, Lucilia sericata

2012 ◽  
Vol 26 (3) ◽  
pp. 291-299 ◽  
Author(s):  
G. TELFORD ◽  
A. P. BROWN ◽  
A. RICH ◽  
J. S. C. ENGLISH ◽  
D. I. PRITCHARD
Keyword(s):  
Wound Healing ◽  
Lucilia Sericata ◽  
Wound Debridement

scholarly journals Effectiveness of Chronic Wound Debridement with the Use of Larvae of Lucilia Sericata

2019 ◽  
Vol 8 (11) ◽  
pp. 1845 ◽  
Author(s):  
Dariusz Bazaliński ◽  
Maria Kózka ◽  
Magdalena Karnas ◽  
Paweł Więch
Keyword(s):  
Chronic Wound ◽  
Scientific Evidence ◽  
Bacterial Load ◽  
Cost Effective ◽  
Tissue Growth ◽  
Lucilia Sericata ◽  
Wound Debridement ◽  
Cost Effective Method ◽  
Phaenicia Sericata ◽  
Maggot Therapy

The process of successful wound healing depends on effective debridement and infection control. One method of wound debridement, known since antiquity, is based on the use of fly larvae. Solid scientific evidence proves that maggot debridement therapy (MDT), like surgical intervention, can be effectively and safely used to remove necrotic tissue. Based on a review of the related literature, this study was designed to assess the effectiveness of chronic wound cleansing with the use of larvae of Lucilia sericata (Phaenicia sericata). Maggot therapy, applied in wound debridement and treatment, is a safe and effective method. Its benefits are associated with debridement, disinfection and faster tissue growth. MDT may reduce the duration of antibiotic therapy and the need for hospitalization, or it may decrease the number of outpatient visits required. It is a relatively cost-effective method, and, in addition to financial gains, it may reduce the frequency of inpatient treatment. In the literature, an increasing amount of scientific evidence confirms that such treatment can effectively reduce the biofilm and bacterial load in a wound.

A Jonah-like chymotrypsin from the therapeutic maggot Lucilia sericata plays a role in wound debridement and coagulation

2016 ◽  
Vol 70 ◽  
pp. 138-147 ◽  
Author(s):  
Anne-Kathrin Pöppel ◽  
Mareike Kahl ◽  
Andre Baumann ◽  
Jochen Wiesner ◽  
Anke Gökçen ◽  
...  
Keyword(s):  
Lucilia Sericata ◽  
Wound Debridement

scholarly journals Surgical Strategies to Promote Cutaneous Healing

2021 ◽  
Vol 9 (2) ◽  
pp. 45
Author(s):  
Ines Maria Niederstätter ◽  
Jennifer Lynn Schiefer ◽  
Paul Christian Fuchs
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Chronic Wounds ◽  
Treatment Algorithm ◽  
Surgical Site Infections ◽  
Repair Process ◽  
Suture Technique ◽  
The Body ◽  
Wound Debridement ◽  
Reconstructive Ladder

Usually, cutaneous wound healing does not get impeded and processes uneventfully, reaching wound closure easily. The goal of this repair process is to restore the integrity of the body surface by creating a resilient and stable scar. Surgical practice and strategies have an impact on the course of wound healing and the later appearance of the scar. By considering elementary surgical principles, such as the appropriate suture material, suture technique, and timing, optimal conditions for wound healing can be created. Wounds can be differentiated into clean wounds, clean–contaminated wounds, contaminated, and infected/dirty wounds, based on the degree of colonization or infection. Furthermore, a distinction is made between acute and chronic wounds. The latter are wounds that persist for longer than 4–6 weeks. Care should be taken to avoid surgical site infections in the management of wounds by maintaining sterile working conditions, using antimicrobial working techniques, and implementing the principles of preoperative antibiotics. Successful wound closure is influenced by wound debridement. Wound debridement removes necrotic tissue, senescent and non-migratory cells, bacteria, and foreign bodies that impede wound healing. Additionally, the reconstructive ladder is a viable and partially overlapping treatment algorithm in plastic surgery to achieve successful wound closure.

Punch Grafting for the Management of Hard-to-Heal Diabetic Foot Ulcers: A Prospective Case Series

2021 ◽  
pp. 153473462110310
Author(s):  
Marta García-Madrid ◽  
Irene Sanz-Corbalán ◽  
Aroa Tardáguila-García ◽  
Raúl J. Molines-Barroso ◽  
Mateo López-Moral ◽  
...  
Keyword(s):  
Wound Healing ◽  
Clinical Outcomes ◽  
Diabetic Foot ◽  
Donor Site ◽  
Case Series ◽  
Foot Ulcers ◽  
Diabetic Foot Ulcers ◽  
Wound Debridement ◽  
Prospective Case Series

Punch grafting is an alternative treatment to enhance wound healing which has been associated with promising clinical outcomes in various leg and foot wound types. We aimed to evaluate the clinical outcomes of punch grafting as a treatment for hard-to-heal diabetic foot ulcers (DFUs). Six patients with chronic neuropathic or neuroischemic DFUs with more than 6 months of evolution not responding to conventional treatment were included in a prospective case series between May 2017 and December 2020. All patients were previously debrided using an ultrasound-assisted wound debridement and then, grafted with 4 to 6 mm punch from the donor site that was in all cases the anterolateral aspect of the thigh. All patients were followed up weekly until wound healing. Four (66.7%) DFUs were located in the heel, 1 (16.7%) in the dorsal aspect of the foot and 1 (16.7%) in the Achilles tendon. The median evolution time was 172 (interquartile range [IQR], 25th-75th; 44-276) weeks with a median area of 5.9 (IQR; 1.87-37.12) cm2 before grafting. Complete epithelization was achieved in 3 (50%) patients at 12 weeks follow-up period with a mean time of 5.67 ± 2.88 weeks. Two of the remaining patients achieved wound healing at 32 and 24 weeks, respectively, and 1 patient showed punch graft unsuccessful in adhering. The median time of wound healing of all patients included in the study was 9.00 (IQR; 4.00-28.00) weeks. The wound area reduction (WAR) at 4 weeks was 38.66% and WAR at 12 weeks was 88.56%. No adverse effects related to the ulcer were registered through the follow-up period. Autologous punch graft is an easy procedure that promotes healing, achieving wound closure in chronic DFUs representing an alternative of treatment for hard-to-heal DFUs in which conservative treatment has been unsuccessful.

Antibacterial substances of low molecular weight isolated from the blowfly, Lucilia sericata

2007 ◽  
Vol 21 (2) ◽  
pp. 127-131 ◽  
Author(s):  
L. HUBERMAN ◽  
N. GOLLOP ◽  
K. Y. MUMCUOGLU ◽  
E. BREUER ◽  
S. R. BHUSARE ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Lucilia Sericata ◽  
Low Molecular Weight
Sign in / Sign up

Export Citation Format

Share Document