scholarly journals Preservation of neutralizing antibody function in COVID‐19 convalescent plasma treated using a riboflavin and ultraviolet light‐based pathogen reduction technology

Vox Sanguinis ◽  
2021 ◽  
Author(s):  
Susan Yonemura ◽  
Lindsay Hartson ◽  
Taru S. Dutt ◽  
Marcela Henao‐Tamayo ◽  
Raymond Goodrich ◽  
...  
Keyword(s):  
Ultraviolet Light ◽  
Neutralizing Antibody ◽  
Pathogen Reduction ◽  
Antibody Function

scholarly journals Preservation of neutralizing antibody function in COVID-19 convalescent plasma treated using a riboflavin and ultraviolet light-based pathogen reduction technology

2021 ◽  
Author(s):  
Susan Yonemura ◽  
Lindsay Hartson ◽  
Taru Dutt ◽  
Marcela Henao-Tamayo ◽  
Raymond Goodrich ◽  
...  
Keyword(s):  
Ultraviolet Light ◽  
Therapeutic Option ◽  
Transmitted Disease ◽  
Coagulation Factor ◽  
Neutralizing Antibody ◽  
Coagulation Factors ◽  
Post Treatment ◽  
Protein Receptor ◽  
Pathogen Reduction ◽  
Antibody Function

AbstractBackground and ObjectiveConvalescent plasma (CP) has been embraced as a safe therapeutic option for coronavirus disease 2019 (COVID-19) while other treatments are developed. However, transfusion-transmitted disease is a risk, particularly in regions with high endemic prevalence of transfusion-transmissible diseases. Pathogen reduction can mitigate this risk; thus, the objective of this study was to evaluate the effect of riboflavin and ultraviolet light (R+UV) pathogen reduction technology on the functional properties of CCP.Materials and MethodsCCP units (n = 6) from recovered COVID-19 research donors were treated with R+UV. Pre- and post-treatment samples were tested for coagulation factor and immunoglobulin retention. Antibody binding to spike protein receptor binding domain (RBD), S1, and S2 epitopes of SARS-CoV-2 was assessed by ELISA.Neutralizing antibody (nAb) function was assessed by pseudovirus reporter viral particle neutralization (RVPN) assay and plaque reduction neutralization test (PRNT).ResultsMean retention of coagulation factors was ≥ 70% while retention of immunoglobulins was 100%. Starting nAb titers were low, but PRNT50 titers did not differ between pre- and post-treatment samples. No statistically significant differences were detected in levels of IgG (P ≥ 0.3665) and IgM (P ≥ 0.1208) antibodies to RBD, S1, and S2 proteins before and after treatment.ConclusionR+UV PRT effects on coagulation factors were similar to previous reports, but no significant effects were observed on immunoglobulin concentration and antibody function. SARS-CoV-2 nAb function in COVID-19 convalescent plasma is conserved following R+UV PRT treatment.

Effects of riboflavin and ultraviolet light treatment on pathogen reduction and platelets

Transfusion ◽  
2020 ◽  
Vol 60 (11) ◽  
pp. 2647-2654
Author(s):  
Yundi Yin ◽  
Ling Li ◽  
Li Gong ◽  
Haixia Xu ◽  
Zhong Liu
Keyword(s):  
Ultraviolet Light ◽  
Light Treatment ◽  
Pathogen Reduction

Riboflavin-ultraviolet light pathogen reduction treatment does not impact the immunogenicity of murine red blood cells

Transfusion ◽  
2015 ◽  
Vol 56 (4) ◽  
pp. 863-872 ◽  
Author(s):  
Christopher A. Tormey ◽  
Manjula Santhanakrishnan ◽  
Nicole H. Smith ◽  
Jingchun Liu ◽  
Susanne Marschner ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Ultraviolet Light ◽  
Blood Cells ◽  
Reduction Treatment ◽  
Pathogen Reduction

scholarly journals Impact of pathogen reduction technology on neutralizing antibody titer in COVID-19 convalescent plasma

2021 ◽  
Author(s):  
Massimo Franchini ◽  
Fausto Baldanti ◽  
Elena Percivalle ◽  
Alessandro Ferrari ◽  
Pietro Biasi ◽  
...  
Keyword(s):  
Antibody Titer ◽  
Neutralizing Antibody ◽  
Pathogen Reduction

Two pathogen reduction technologies-methylene blue plus light and shortwave ultraviolet light-effectively inactivate hepatitis C virus in blood products

Transfusion ◽  
2012 ◽  
Vol 53 (5) ◽  
pp. 1010-1018 ◽  
Author(s):  
Eike Steinmann ◽  
Ute Gravemann ◽  
Martina Friesland ◽  
Juliane Doerrbecker ◽  
Thomas H. Müller ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Ultraviolet Light ◽  
Blood Products ◽  
Pathogen Reduction

scholarly journals Pathogen reduction with riboflavin and ultraviolet light induces a quasi‐apoptotic state in blood leukocytes

Transfusion ◽  
2019 ◽  
Vol 59 (11) ◽  
pp. 3501-3510 ◽  
Author(s):  
Johnson Q. Tran ◽  
Marcus O. Muench ◽  
John W. Heitman ◽  
Rachael P. Jackman
Keyword(s):  
Ultraviolet Light ◽  
Blood Leukocytes ◽  
Pathogen Reduction

scholarly journals Our experience in riboflavin and ultraviolet light pathogen reduction technology for platelets: from platelet production to patient care

Transfusion ◽  
2018 ◽  
Vol 58 (8) ◽  
pp. 1881-1889 ◽  
Author(s):  
Teresa Jimenez-Marco ◽  
Marta Garcia-Recio ◽  
Enrique Girona-Llobera
Keyword(s):  
Patient Care ◽  
Ultraviolet Light ◽  
Platelet Production ◽  
Pathogen Reduction

Pathogen reduction of fresh plasma using riboflavin and ultraviolet light: effects on plasma coagulation proteins

Transfusion ◽  
2009 ◽  
Vol 49 (10) ◽  
pp. 2167-2172 ◽  
Author(s):  
Valerie S. Hornsey ◽  
Olive Drummond ◽  
Alex Morrison ◽  
Loraine McMillan ◽  
Ian R. MacGregor ◽  
...  
Keyword(s):  
Ultraviolet Light ◽  
Plasma Coagulation ◽  
Coagulation Proteins ◽  
Fresh Plasma ◽  
Light Effects ◽  
Pathogen Reduction

scholarly journals A New SARS CoV-2 Dual Purpose Serology Test: Highly Accurate Infection Tracing and Neutralizing Antibody Response Detection

2021 ◽  
Author(s):  
Sean C. Taylor ◽  
Beth Hurst ◽  
Carmen L. Charlton ◽  
Ashley Bailey ◽  
Jamil N. Kanji ◽  
...  
Keyword(s):  
Neutralizing Antibody ◽  
Live Cell ◽  
Dual Purpose ◽  
Protein Receptor ◽  
Serology Test ◽  
Response Detection ◽  
High Throughput Assay ◽  
Improved Accuracy ◽  
Antibody Function ◽  
Combined Data

Many SARS CoV-2 serology tests have proven to be less accurate than expected and do not assess antibody function as neutralizing, correlating with protection from reinfection. A new assay technology measuring the interaction of purified SARS CoV-2 spike protein receptor binding domain (RBD) with the extracellular domain of the human ACE2 receptor detects these important antibodies. This cPassTM surrogate virus neutralization test (sVNT) when compared directly with eight SARS CoV-2 IgG serology and two live cell neutralization tests gives similar or improved accuracy for qualitative delineation between positive and negative individuals in a fast, scalable and high throughput assay. The combined data support cPassTM sVNT as a tool for highly accurate SARS CoV-2 immunity surveillance of infected/recovered and/or vaccinated individuals, as well as drug and convalescent donor screening. The data also prevue a novel application for cPassTM sVNT in calibrating the stringency of live cell neutralization tests and its use in longitudinal testing of recovered and/or vaccinated patients.

Riboflavin and ultraviolet light for pathogen reduction of murine cytomegalovirus in blood products

Transfusion ◽  
2014 ◽  
Vol 55 (4) ◽  
pp. 858-863 ◽  
Author(s):  
Shawn D. Keil ◽  
Natia Saakadze ◽  
Richard Bowen ◽  
James L. Newman ◽  
Sulaiman Karatela ◽  
...  
Keyword(s):  
Ultraviolet Light ◽  
Murine Cytomegalovirus ◽  
Blood Products ◽  
Pathogen Reduction
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