scholarly journals GMP‐grade CD34 + selection from HLA‐homozygous licensed cord blood units and short‐term expansion under European ATMP regulations

Vox Sanguinis ◽  
2020 ◽  
Author(s):  
Stefanie Liedtke ◽  
Lutz Korschgen ◽  
Janine Korn ◽  
Almuth Duppers ◽  
Gesine Kogler
Keyword(s):  
Cord Blood ◽  
Short Term ◽  
Cd34 Selection

Short‐Term exposure of umbilical cord blood CD34 + cells to human platelet lysate and cytokines enhances engraftment

Transfusion ◽  
2020 ◽  
Vol 60 (10) ◽  
pp. 2348-2358
Author(s):  
Marie‐Ève Rhéaume ◽  
Pascal Rouleau ◽  
Tony Tremblay ◽  
Isabelle Paré ◽  
Lionel Loubaki
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Short Term ◽  
Short Term Exposure ◽  
Cd34 Cells ◽  
Cord Blood Cd34 ◽  
Human Platelet Lysate

scholarly journals Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture

2012 ◽  
Vol 42 (2) ◽  
pp. 155-160 ◽  
Author(s):  
Céline Borlon ◽  
Bruce Russell ◽  
Kanlaya Sriprawat ◽  
Rossarin Suwanarusk ◽  
Annette Erhart ◽  
...  
Keyword(s):  
Cord Blood ◽  
Plasmodium Vivax ◽  
Short Term ◽  
Short Term Culture

Short-Term Culture of Umbilical Cord Blood–Derived CD34 Cells Enhances Engraftment Into NOD/SCID Mice Through Increased CXCR4 Expression

2009 ◽  
Vol 18 (8) ◽  
pp. 1221-1226 ◽  
Author(s):  
Norioki Ohno ◽  
Teruyuki Kajiume ◽  
Yasuhiko Sera ◽  
Takashi Sato ◽  
Masao Kobayashi
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cxcr4 Expression ◽  
Scid Mice ◽  
Short Term ◽  
Cd34 Cells ◽  
Short Term Culture

Delayed Engraftment of Nonobese Diabetic/Severe Combined Immunodeficient Mice Transplanted With Ex Vivo–Expanded Human CD34+ Cord Blood Cells

Blood ◽  
1999 ◽  
Vol 93 (3) ◽  
pp. 1097-1105 ◽  
Author(s):  
G. Güenechea ◽  
J.C. Segovia ◽  
B. Albella ◽  
M. Lamana ◽  
M. Ramı́rez ◽  
...  
Keyword(s):  
Cord Blood ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Hematopoietic Progenitors ◽  
Short Term ◽  
Immunodeficient Mice ◽  
Nonobese Diabetic ◽  
Cd34 Cells

Abstract The ex vivo expansion of hematopoietic progenitors is a promising approach for accelerating the engraftment of recipients, particularly when cord blood (CB) is used as a source of hematopoietic graft. With the aim of defining the in vivo repopulating properties of ex vivo–expanded CB cells, purified CD34+ cells were subjected to ex vivo expansion, and equivalent proportions of fresh and ex vivo–expanded samples were transplanted into irradiated nonobese diabetic (NOD)/severe combined immunodeficient (SCID) mice. At periodic intervals after transplantation, femoral bone marrow (BM) samples were obtained from NOD/SCID recipients and the kinetics of engraftment evaluated individually. The transplantation of fresh CD34+ cells generated a dose-dependent engraftment of recipients, which was evident in all of the posttransplantation times analyzed (15 to 120 days). When compared with fresh CB, samples stimulated for 6 days with interleukin-3 (IL-3)/IL-6/stem cell factor (SCF) contained increased numbers of hematopoietic progenitors (20-fold increase in colony-forming unit granulocyte-macrophage [CFU-GM]). However, a significant impairment in the short-term repopulation of recipients was associated with the transplantation of the ex vivo–expanded versus the fresh CB cells (CD45+repopulation in NOD/SCIDs BM: 3.7% ± 1.2% v 26.2% ± 5.9%, respectively, at 20 days posttransplantation; P < .005). An impaired short-term engraftment was also observed in mice transplanted with CB cells incubated with IL-11/SCF/FLT-3 ligand (3.5% ± 1.7% of CD45+ cells in femoral BM at 20 days posttransplantation). In contrast to these data, a similar repopulation with the fresh and the ex vivo–expanded cells was observed at later stages posttransplantation. At 120 days, the repopulation of CD45+ and CD45+/CD34+ cells in the femoral BM of recipients ranged between 67.2% to 81.1% and 8.6% to 12.6%, respectively, and no significant differences of engraftment between recipients transplanted with fresh and the ex vivo–expanded samples were found. The analysis of the engrafted CD45+ cells showed that both the fresh and the in vitro–incubated samples were capable of lymphomyeloid reconstitution. Our results suggest that although the ex vivo expansion of CB cells preserves the long-term repopulating ability of the sample, an unexpected delay of engraftment is associated with the transplantation of these manipulated cells.

Hematopoietic activity of human short-term repopulating cells in mobilized peripheral blood cell transplants is restricted to the first 5 months after transplantation

Blood ◽  
2010 ◽  
Vol 115 (24) ◽  
pp. 5023-5025 ◽  
Author(s):  
Oksana Zavidij ◽  
Claudia R. Ball ◽  
Friederike Herbst ◽  
Sylvia Fessler ◽  
Manfred Schmidt ◽  
...  
Keyword(s):  
Cord Blood ◽  
Peripheral Blood ◽  
Cell Types ◽  
Peripheral Blood Cell ◽  
Short Term ◽  
Cell Transplants ◽  
Stem And Progenitor Cells ◽  
Quantitative Contribution ◽  
Kinetics Of ◽  
Mobilized Peripheral Blood

Abstract Kinetics of hematopoietic recovery driven by different types of human stem and progenitor cells after transplantation are not fully understood. Short-term repopulating cells (STRCs) dominate early hematopoiesis after transplantation. STRCs are highly enriched in adult mobilized peripheral blood compared with cord blood, but the length of their contribution to hematopoiesis remains unclear. To understand posttransplantation durability and lineage contribution of STRCs, we compared repopulation kinetics of mobilized peripheral blood (high STRC content) with cord blood transplants (low STRC content) in long-lived NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (IL2RG−/−) mice. This comparison demonstrates that quantitative contribution of human STRCs to hematopoiesis is restricted to the first 5 months after transplantation. The ratio of STRCs to long-term repopulating cells dramatically changes during ontogeny. This model enables to precisely determine early and late engraftment kinetics of defined human repopulating cell types and to preclinically assess the engraftment kinetics of engineered stem cell transplants.

scholarly journals Comparative study of the in vitro behavior of cord blood subpopulations after short-term cytokine exposure

1999 ◽  
Vol 23 (3) ◽  
pp. 211-220 ◽  
Author(s):  
A Rice ◽  
C Flemming ◽  
J Case ◽  
J Stevenson ◽  
L Gaudry ◽  
...  
Keyword(s):  
Comparative Study ◽  
Cord Blood ◽  
Short Term
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