Short-Term Culture of Umbilical Cord Blood–Derived CD34 Cells Enhances Engraftment Into NOD/SCID Mice Through Increased CXCR4 Expression

2009 ◽  
Vol 18 (8) ◽  
pp. 1221-1226 ◽  
Author(s):  
Norioki Ohno ◽  
Teruyuki Kajiume ◽  
Yasuhiko Sera ◽  
Takashi Sato ◽  
Masao Kobayashi
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cxcr4 Expression ◽  
Scid Mice ◽  
Short Term ◽  
Cd34 Cells ◽  
Short Term Culture

Short‐Term exposure of umbilical cord blood CD34 + cells to human platelet lysate and cytokines enhances engraftment

Transfusion ◽  
2020 ◽  
Vol 60 (10) ◽  
pp. 2348-2358
Author(s):  
Marie‐Ève Rhéaume ◽  
Pascal Rouleau ◽  
Tony Tremblay ◽  
Isabelle Paré ◽  
Lionel Loubaki
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Short Term ◽  
Short Term Exposure ◽  
Cd34 Cells ◽  
Cord Blood Cd34 ◽  
Human Platelet Lysate

scholarly journals Engraftment and Development of Human CD34+-Enriched Cells From Umbilical Cord Blood in NOD/LtSz-scid/scid Mice

Blood ◽  
1997 ◽  
Vol 90 (1) ◽  
pp. 85-96 ◽  
Author(s):  
Christopher J. Hogan ◽  
Elizabeth J. Shpall ◽  
Oren McNulty ◽  
Ian McNiece ◽  
John E. Dick ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Progenitor Cell ◽  
Scid Mice ◽  
Hematopoietic Progenitor Cell ◽  
Developmental Potential ◽  
Cd34 Cells ◽  
Cell Fractions

Abstract Understanding the repopulating characteristics of human hematopoietic stem/progenitor cell fractions is crucial for predicting their performance after transplant into high-risk patients following high-dose therapy. We report that human umbilical cord blood cells, 78% to 100% of which express the hematopoietic progenitor cell surface marker CD34, can consistently engraft, develop, and proliferate in the hematopoietic tissues of sublethally irradiated NOD/LtSz-scid/scid mice. Engraftment and development of CD34+ cells is not dependent on human growth factor support. CD34+ cells home to the mouse bone marrow (BM) that becomes the primary site of human hematopoietic development containing myeloid, lymphoid, erythroid, and CD34+ progenitor populations. Myeloid, and in particular lymphoid cells possessing more mature cell surface markers, comprise the human component of mouse spleen and peripheral blood, indicating that development proceeds from primary hematopoietic sites to the periphery. Repopulation of secondary recipients with human cells by BM from primary recipients demonstrates the maintenance of substantial proliferation capacity of the input precursor population. These data suggest that the cells capable of initiating human cell engraftment (SCID-repopulating cells) are contained in the CD34+ cell fraction, and that this mouse model will be useful for assaying the developmental potential of other rare human hematopoietic cell fractions in vivo.

Similar repopulating capacity of mitotically active and resting umbilical cord blood CD34+ cells in NOD/SCID mice

Blood ◽  
2000 ◽  
Vol 96 (6) ◽  
pp. 2100-2107 ◽  
Author(s):  
Jannine Wilpshaar ◽  
J. H. Frederik Falkenburg ◽  
Xia Tong ◽  
Willy A. Noort ◽  
Robert Breese ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Linear Models ◽  
Human Cells ◽  
Scid Mice ◽  
Mammalian Development ◽  
Hematopoietic Stem ◽  
Cd34 Cells ◽  
Cord Blood Cd34

It was hypothesized that during mammalian development, the extensive need for hematopoietic cells requires equal contribution to blood cell production from both quiescent and cycling hematopoietic stem cells (HSCs) while maintaining the stem cell pool. To investigate this hypothesis, the engraftment potential of umbilical cord blood (UCB) CD34+ cells residing in either G0(G0CD34+ cells) or G1(G1CD34+ cells) phases of the cell cycle was assessed in nonobese diabetic/severe combined immune-deficient (NOD/SCID) mice. Whereas the level of chimerism in mice transplanted with UCB G0CD34+ cells was 69.9% ± 24.0%, mice receiving equal numbers of G1CD34+ cells harbored 46.7% ± 21.3% human cells 8 weeks posttransplantation. Both groups of cells sustained multilineage differentiation and the production of CD34+cells in recipient animals. The relationship between the number of transplanted G0CD34+ or G1CD34+ cells and the level of chimerism was analyzed by a general linear models procedure. Although the initial level of chimerism following transplantation of G0CD34+ cells was higher than that sustained by G1CD34+ cells, the increment in the degree of chimerism obtained with each additional 103 cells of either phenotype was identical, suggesting that the reconstitution potential of these 2 types of cells was similar. Of interest is that human cells recovered from primary recipients of both G0CD34+ and G1CD34+cells engrafted in secondary NOD/SCID recipients, albeit at a substantially lower level, confirming the primitive nature of UCB CD34+ cells residing in G1.

Mesenchymal stem cells promote engraftment of human umbilical cord blood–derived CD34+ cells in NOD/SCID mice

2002 ◽  
Vol 30 (8) ◽  
pp. 870-878 ◽  
Author(s):  
Willy A Noort ◽  
Alwine B Kruisselbrink ◽  
Pieternella S in't Anker ◽  
Marjolein Kruger ◽  
Rutger L van Bezooijen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Scid Mice ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Cd34 Cells

scholarly journals CXCR4 expression in CD34+ cells and unit predominance after double umbilical cord blood transplantation

Leukemia ◽  
2012 ◽  
Vol 27 (5) ◽  
pp. 1181-1183 ◽  
Author(s):  
P Ramirez ◽  
J E Wagner ◽  
T E DeFor ◽  
C R Eide ◽  
J S Miller ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cord Blood Transplantation ◽  
Cxcr4 Expression ◽  
Umbilical Cord Blood Transplantation ◽  
Blood Transplantation ◽  
Cd34 Cells

Nonexpanded primary lung and bone marrow–derived mesenchymal cells promote the engraftment of umbilical cord blood–derived CD34+ cells in NOD/SCID mice

2003 ◽  
Vol 31 (10) ◽  
pp. 881-889 ◽  
Author(s):  
Pieternella S in 't Anker ◽  
Willy A Noort ◽  
Alwine B Kruisselbrink ◽  
Sicco A Scherjon ◽  
Willem Beekhuizen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Mesenchymal Cells ◽  
Scid Mice ◽  
Cd34 Cells

Enhanced In Vivo Homing of Uncultured and Selectively Amplified Umbilical Cord Blood CD34+ Cells by Co-Transplantation with Cord Blood Derived Unrestricted Somatic Stem Cells.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 1276-1276
Author(s):  
Shing Leng Chan ◽  
Michael S.K. Choi ◽  
Stephan Wnendt ◽  
Morey Kraus ◽  
Eileen Teng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Scid Mice ◽  
Somatic Stem Cells ◽  
Cd34 Cells ◽  
Cord Blood Cd34 ◽  
Unrestricted Somatic Stem Cells

Abstract Mesenchymal stem cells have been implicated as playing an important role in stem cell engraftment. A new pluripotent population of umbilical cord blood (UCB)-derived Unrestricted Somatic Stem Cells (USSCs), with intrinsic and directable potential to develop into mesodermal, endodermal and ectodermal fates, has recently been identified and characterized (1). In this study we evaluated the capacity of USSCs to influence the homing of UCB -derived CD34+ cells into the marrow and spleen of NOD/SCID mice. Cultured USSCs were co-transplanted with CFDA-labeled cord blood CD34+ cells into sublethally irradiated NOD/SCID mice. Femurs and spleens were harvested 16 hrs thereafter and the percentage of CD34+ cells determined by flow cytometry. USSCs induced a significant enhancement of CD34+ cell homing to both bone marrow and spleen (2.2 ± 0.3 and 2.4 ± 0.6 -fold, respectively; p<0.05). Similar findings were obtained with frozen USSC samples that had been thawed prior to transplantation. The effect of USSCs was specific, as no homing enhancement could be observed by co-transplantation of CD34+ cells with lineage-positive UCB cells, which contain T, B and mature myelo-erythroid cells. Enhanced marrow homing by USSCs was unaltered by extensive culture passaging of the cells, with a similar degree of enhancement observed for both early (p5) and late (p10) passage USSCs (1.7 ± 0.1 and 1.9 ± 0.1 -fold, respectively). Enhanced homing was dose-dependent, detectable at USSC/CD34+ ratios of 1:1 and above. USSCs were also found to enhance the homing of day 14 cells harvested from cultures of selectively amplifiedTM, ex-vivo expanded UCB lineage-negative (lin-) cells. The relative proportion of homing CD34+ cells within the culture-expanded cell population, was unaffected by USSC co-transplantation. Our findings thus demonstrate that USSCs enhance the homing of UCB hematopoietic stem cells and suggest a clinical potential for these cells in facilitating engraftment under conditions of limiting cord blood stem cell numbers.

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