Loss of the ability to generate large burst-forming unit-like megakaryocytic colonies from thawed cord blood in semisolid cultures after short term suspension culture

Vox Sanguinis ◽  
2014 ◽  
Vol 108 (3) ◽  
pp. 294-301
Author(s):  
M. Eskola ◽  
S. Bäckman ◽  
S. Möttönen ◽  
R. Kekomäki
Keyword(s):  
Suspension Culture ◽  
Cord Blood ◽  
Short Term ◽  
Large Burst

scholarly journals Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin C

2021 ◽  
Vol 11 (4) ◽  
pp. 1941
Author(s):  
Seungmin Yeom ◽  
Myung Chul Lee ◽  
Shambhavi Pandey ◽  
Jaewoon Lim ◽  
Sangbae Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Shear Stress ◽  
Stem Cell ◽  
Vitamin C ◽  
Suspension Culture ◽  
Small Molecules ◽  
Tumor Development ◽  
Embryonic Stem ◽  
Short Term ◽  
External Stimuli

Induced pluripotent stem cells (iPSCs) are a good medicine source because of their potential to differentiate into various tissues or cells. However, traditionally, iPSCs made by specific transgenes and virus vectors are not appropriate for clinical use because of safety concerns and risk of tumor development. The goal of this research was to develop an alternative method for reprogramming, using small molecules and external stimuli. Two groups were established: short-term shear stress (STSS) under suspension culture and a combination of short-term shear stress and vitamin C (SSVC) under suspension culture. For STSS, the pipetting was carried out for cells twice per day for 2 min for 14 days in the embryonic stem cell (ES) medium. In the case of SSVC, the procedure was the same as for STSS however, its ES medium included 10 µM of vitamin C. After 14 days, all spheroids were picked and checked for pluripotency by ALP (alkaline phosphatase) assay and immunocytochemistry. Both groups partially showed the characteristics of stem cells but data demonstrated that the spheroids under shear stress and vitamin C had improved stem cell-like properties. This research showed the possibility of external stimuli and small molecules to reprogram the somatic cells without the use of transgenes.

scholarly journals A simple in vitro method for the assessment of ineffective erythropoiesis

Blood ◽  
1981 ◽  
Vol 58 (4) ◽  
pp. 782-787 ◽  
Author(s):  
D Samson ◽  
J Tikerpae ◽  
H Crowne
Keyword(s):  
Suspension Culture ◽  
Cell Counting ◽  
Normal Range ◽  
Ineffective Erythropoiesis ◽  
Short Term ◽  
In Vitro Method ◽  
Hematologic Disorders

Abstract A simple in vitro method has been developed for the assessment of ineffective erythropoiesis by measuring the release of heme from a labeled cohort of erythroblasts in short-term suspension culture. The release of labeled heme was shown to correlate with the death of erythroblasts in culture determined by cell counting. Heme release was markedly increased in conditions where there is known to be excessive ineffective erythropoiesis, while in hematologic disorders where ineffective erythropoiesis is thought to be normal, heme release was within the normal range.

Short‐Term exposure of umbilical cord blood CD34 + cells to human platelet lysate and cytokines enhances engraftment

Transfusion ◽  
2020 ◽  
Vol 60 (10) ◽  
pp. 2348-2358
Author(s):  
Marie‐Ève Rhéaume ◽  
Pascal Rouleau ◽  
Tony Tremblay ◽  
Isabelle Paré ◽  
Lionel Loubaki
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Short Term ◽  
Short Term Exposure ◽  
Cd34 Cells ◽  
Cord Blood Cd34 ◽  
Human Platelet Lysate

scholarly journals Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture

2012 ◽  
Vol 42 (2) ◽  
pp. 155-160 ◽  
Author(s):  
Céline Borlon ◽  
Bruce Russell ◽  
Kanlaya Sriprawat ◽  
Rossarin Suwanarusk ◽  
Annette Erhart ◽  
...  
Keyword(s):  
Cord Blood ◽  
Plasmodium Vivax ◽  
Short Term ◽  
Short Term Culture

Short-Term Culture of Umbilical Cord Blood–Derived CD34 Cells Enhances Engraftment Into NOD/SCID Mice Through Increased CXCR4 Expression

2009 ◽  
Vol 18 (8) ◽  
pp. 1221-1226 ◽  
Author(s):  
Norioki Ohno ◽  
Teruyuki Kajiume ◽  
Yasuhiko Sera ◽  
Takashi Sato ◽  
Masao Kobayashi
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cxcr4 Expression ◽  
Scid Mice ◽  
Short Term ◽  
Cd34 Cells ◽  
Short Term Culture

Delayed Engraftment of Nonobese Diabetic/Severe Combined Immunodeficient Mice Transplanted With Ex Vivo–Expanded Human CD34+ Cord Blood Cells

Blood ◽  
1999 ◽  
Vol 93 (3) ◽  
pp. 1097-1105 ◽  
Author(s):  
G. Güenechea ◽  
J.C. Segovia ◽  
B. Albella ◽  
M. Lamana ◽  
M. Ramı́rez ◽  
...  
Keyword(s):  
Cord Blood ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Hematopoietic Progenitors ◽  
Short Term ◽  
Immunodeficient Mice ◽  
Nonobese Diabetic ◽  
Cd34 Cells

Abstract The ex vivo expansion of hematopoietic progenitors is a promising approach for accelerating the engraftment of recipients, particularly when cord blood (CB) is used as a source of hematopoietic graft. With the aim of defining the in vivo repopulating properties of ex vivo–expanded CB cells, purified CD34+ cells were subjected to ex vivo expansion, and equivalent proportions of fresh and ex vivo–expanded samples were transplanted into irradiated nonobese diabetic (NOD)/severe combined immunodeficient (SCID) mice. At periodic intervals after transplantation, femoral bone marrow (BM) samples were obtained from NOD/SCID recipients and the kinetics of engraftment evaluated individually. The transplantation of fresh CD34+ cells generated a dose-dependent engraftment of recipients, which was evident in all of the posttransplantation times analyzed (15 to 120 days). When compared with fresh CB, samples stimulated for 6 days with interleukin-3 (IL-3)/IL-6/stem cell factor (SCF) contained increased numbers of hematopoietic progenitors (20-fold increase in colony-forming unit granulocyte-macrophage [CFU-GM]). However, a significant impairment in the short-term repopulation of recipients was associated with the transplantation of the ex vivo–expanded versus the fresh CB cells (CD45+repopulation in NOD/SCIDs BM: 3.7% ± 1.2% v 26.2% ± 5.9%, respectively, at 20 days posttransplantation; P < .005). An impaired short-term engraftment was also observed in mice transplanted with CB cells incubated with IL-11/SCF/FLT-3 ligand (3.5% ± 1.7% of CD45+ cells in femoral BM at 20 days posttransplantation). In contrast to these data, a similar repopulation with the fresh and the ex vivo–expanded cells was observed at later stages posttransplantation. At 120 days, the repopulation of CD45+ and CD45+/CD34+ cells in the femoral BM of recipients ranged between 67.2% to 81.1% and 8.6% to 12.6%, respectively, and no significant differences of engraftment between recipients transplanted with fresh and the ex vivo–expanded samples were found. The analysis of the engrafted CD45+ cells showed that both the fresh and the in vitro–incubated samples were capable of lymphomyeloid reconstitution. Our results suggest that although the ex vivo expansion of CB cells preserves the long-term repopulating ability of the sample, an unexpected delay of engraftment is associated with the transplantation of these manipulated cells.

Hematopoietic activity of human short-term repopulating cells in mobilized peripheral blood cell transplants is restricted to the first 5 months after transplantation

Blood ◽  
2010 ◽  
Vol 115 (24) ◽  
pp. 5023-5025 ◽  
Author(s):  
Oksana Zavidij ◽  
Claudia R. Ball ◽  
Friederike Herbst ◽  
Sylvia Fessler ◽  
Manfred Schmidt ◽  
...  
Keyword(s):  
Cord Blood ◽  
Peripheral Blood ◽  
Cell Types ◽  
Peripheral Blood Cell ◽  
Short Term ◽  
Cell Transplants ◽  
Stem And Progenitor Cells ◽  
Quantitative Contribution ◽  
Kinetics Of ◽  
Mobilized Peripheral Blood

Abstract Kinetics of hematopoietic recovery driven by different types of human stem and progenitor cells after transplantation are not fully understood. Short-term repopulating cells (STRCs) dominate early hematopoiesis after transplantation. STRCs are highly enriched in adult mobilized peripheral blood compared with cord blood, but the length of their contribution to hematopoiesis remains unclear. To understand posttransplantation durability and lineage contribution of STRCs, we compared repopulation kinetics of mobilized peripheral blood (high STRC content) with cord blood transplants (low STRC content) in long-lived NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (IL2RG−/−) mice. This comparison demonstrates that quantitative contribution of human STRCs to hematopoiesis is restricted to the first 5 months after transplantation. The ratio of STRCs to long-term repopulating cells dramatically changes during ontogeny. This model enables to precisely determine early and late engraftment kinetics of defined human repopulating cell types and to preclinically assess the engraftment kinetics of engineered stem cell transplants.

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