A new transfectant panel cell line-based MoAb-independent antigen capture assay system for detection of CD36 antibody

Vox Sanguinis ◽  
2014 ◽  
Vol 106 (4) ◽  
pp. 368-371 ◽  
Author(s):  
E. Amakishi ◽  
T. Hayashi ◽  
Y. Koh ◽  
N. Matsuyama ◽  
H. Ishii ◽  
...  
Keyword(s):  
Cell Line ◽  
Assay System ◽  
Capture Assay ◽  
Antigen Capture ◽  
Panel Cell

scholarly journals A NEW TRANSFECTANT PANEL CELL LINE-BASED MOAB-INDEPENDENT ANTIGEN CAPTURE ASSAY SYSTEM FOR DETECTION OF CD36 ANTIBODY

2014 ◽  
Vol 60 (6) ◽  
pp. 609-613
Author(s):  
Etsuko Amakishi ◽  
Tomoya Hayashi ◽  
Yangsook Koh ◽  
Nobuki Matsuyama ◽  
Hiroyuki Ishii ◽  
...  
Keyword(s):  
Cell Line ◽  
Assay System ◽  
Capture Assay ◽  
Antigen Capture ◽  
Panel Cell

scholarly journals Diagnosis of malaria by detection of Plasmodium falciparum HRP-2 antigen with a rapid dipstick antigen-capture assay

The Lancet ◽  
1994 ◽  
Vol 343 (8897) ◽  
pp. 564-568 ◽  
Author(s):  
C Beadle ◽  
G.W Long ◽  
P.D McElroy ◽  
S.L Hoffman ◽  
G.W Long ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Capture Assay ◽  
Antigen Capture

ACIDIFICATION MODIFIED P24 ANTIGEN CAPTURE ASSAY IN HIV-SEROPOSITIVE PATIENTS

1992 ◽  
Vol 85 (Supplement) ◽  
pp. 3S-42
Author(s):  
David Ascher ◽  
Chet Roberts ◽  
Arnold Fowler
Keyword(s):  
Capture Assay ◽  
Antigen Capture ◽  
P24 Antigen ◽  
Hiv Seropositive

Hybridoma Screening by Antigen Capture: Capture or Sandwich ELISA in 96-Well Plates

2022 ◽  
Vol 2022 (1) ◽  
pp. pdb.prot103127
Author(s):  
Edward A. Greenfield
Keyword(s):  
Culture Supernatant ◽  
Detection Method ◽  
Specific Activity ◽  
Binding Capacity ◽  
Sandwich Elisa ◽  
High Specific Activity ◽  
Tissue Culture Supernatant ◽  
Capture Assay ◽  
Antigen Capture ◽  
Elisa Plate

In an antigen capture assay for hybridoma screening, the detection method identifies the presence of the antigen. Often this is achieved by labeling the antigen directly. In this assay, the polyvinyl chloride (PVC) wells of a high-binding-capacity ELISA plate are first coated with an affinity-purified rabbit anti-mouse immunoglobulin and then incubated with hybridoma tissue culture supernatant. Monoclonal antibodies in the supernatant are “captured” on the coated PVC surface and detected by screening with biotin- or histidine (His)–tagged antigen. The antigen can be labeled to a high specific activity and thus very little antigen is required for this procedure.

scholarly journals A New Range of Chitosan Based Nano-antiviral Agents

2021 ◽  
Vol 13 (1-2) ◽  
Author(s):  
Julio Garay-Jimenez
Keyword(s):  
Antiviral Activity ◽  
Antiviral Agents ◽  
Coxsackie Virus ◽  
Autoimmune Myocarditis ◽  
Capture Assay ◽  
Antigen Capture ◽  
Immunodeficiency Virus ◽  
P24 Antigen ◽  
Syncytial Virus ◽  
Hiv 1

ABSTRACT The current study involves the synthesis of fourteen analogs of oligochitosan and their screening for antiviral potential against human immunodeficiency virus (HIV), respiratory syncytial virus (RSV) and Coxsackie virus. The synthesized oligochitosan analogs were characterized by nuclear magnetic resonance (NMR) and FTIR techniques. HIV-1 p24 ELISA was performed using HIV-1 p24 antigen capture assay in order to estimate the viral infectivity loss. It was observed that sulfated oligochitosan was devoid of antiviral activity as compared to oligochitosan UN102 analog. The rest of UN102 analogs which include N-thiol (UN105), N-glutaryl (UN106), N-Azido (UN111) and N-phthaloyl (UN114) and N-citric analog (UN117) exhibited antiviral activity against HIV. The UN102 also decreased viral infection caused by RSV. In addition, UN102 was found to bind Coxsackie virus, which causes autoimmune myocarditis. The findings were of great interest to proceed for the development of novel antiviral agents.

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