A pilot study documenting increased thrombin generation following abrupt withdrawal of heparin therapy in healthy dogs

2018 ◽  
Vol 28 (6) ◽  
pp. 518-526 ◽  
Author(s):  
Erin M. Mays ◽  
David C. Dorman ◽  
Colleen McKendry ◽  
Rita M. Hanel
Keyword(s):  
Pilot Study ◽  
Thrombin Generation ◽  
Heparin Therapy ◽  
Abrupt Withdrawal ◽  
Healthy Dogs

scholarly journals Persistent Thrombin Generation During Heparin Therapy in Patients With Acute Coronary Syndromes

1997 ◽  
Vol 17 (7) ◽  
pp. 1325-1330 ◽  
Author(s):  
Piera Angelica Merlini ◽  
Diego Ardissino ◽  
Kenneth A. Bauer ◽  
Luigi Oltrona ◽  
Antonio Pezzano ◽  
...  
Keyword(s):  
Acute Coronary Syndromes ◽  
Thrombin Generation ◽  
Heparin Therapy ◽  
Coronary Syndromes

Thrombin generation is increased in patients with Clostridium difficile colitis – a pilot study

Biomarkers ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 389-393
Author(s):  
Romeo G. Mihăilă ◽  
Crina Cătană ◽  
Ariela L. Olteanu ◽  
Victoria Bîrluţiu ◽  
Cosmin Sălcudean ◽  
...  
Keyword(s):  
Pilot Study ◽  
Clostridium Difficile ◽  
Thrombin Generation ◽  
Clostridium Difficile Colitis

scholarly journals Salivary pH, calcium, phosphorus and selected enzymes in healthy dogs: a pilot study

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Ilaria Iacopetti ◽  
Anna Perazzi ◽  
Tamara Badon ◽  
Silvia Bedin ◽  
Barbara Contiero ◽  
...  
Keyword(s):  
Pilot Study ◽  
Salivary Ph ◽  
Calcium Phosphorus ◽  
Healthy Dogs

Reproducibility, stability, and biological variability of thrombin generation using calibrated automated thrombography in healthy dogs

2018 ◽  
Vol 47 (2) ◽  
pp. 218-226 ◽  
Author(s):  
Benoît Cuq ◽  
Shauna L. Blois ◽  
R. Darren Wood ◽  
Gabrielle Monteith ◽  
Anthony C. Abrams-Ogg ◽  
...  
Keyword(s):  
Thrombin Generation ◽  
Biological Variability ◽  
Healthy Dogs

Monitoring unfractionated heparin therapy in dogs by measuring thrombin generation

2011 ◽  
Vol 40 (1) ◽  
pp. 24-31 ◽  
Author(s):  
Virginie Allegret ◽  
Marilyn Dunn ◽  
Christian Bédard
Keyword(s):  
Unfractionated Heparin ◽  
Thrombin Generation ◽  
Heparin Therapy

scholarly journals Expression Profile of Circulating MicroRNAs in Dogs With Cardiac Hypertrophy: A Pilot Study

2021 ◽  
Vol 8 ◽  
Author(s):  
Woong-Bin Ro ◽  
Min-Hee Kang ◽  
Doo-Won Song ◽  
Sung-Hun Lee ◽  
Hee-Myung Park
Keyword(s):  
Pilot Study ◽  
Cardiac Hypertrophy ◽  
Microarray Analysis ◽  
Expression Profile ◽  
Heart Diseases ◽  
Differentially Expressed ◽  
Serum Samples ◽  
Down Regulation ◽  
Circulating Micrornas ◽  
Healthy Dogs

This study aimed to identify the expression profile of circulating microRNAs in dogs with eccentric or concentric cardiac hypertrophy. A total of 291 microRNAs in serum samples of five dogs with myxomatous mitral valve degeneration (MMVD) and five dogs with pulmonic stenosis (PS) were compared with those of five healthy dogs using microarray analysis. Results of microarray analysis revealed up-regulation of cfa-miR-130b [fold change (FC) = 2.13, p = 0.014), down-regulation of cfa-miR-375 (FC = 1.51, p = 0.014), cfa-miR-425 (FC = 2.56, p = 0.045), cfa-miR-30d (FC = 3.02, p = 0.047), cfa-miR-151 (FC = 1.89, p = 0.023), cfa-miR-19b (FC = 3.01, p = 0.008), and cfa-let-7g (FC = 2.53, p = 0.015) in MMVD group which showed eccentric cardiac hypertrophy, up-regulation of cfa-miR-346 (FC = 2.74, p = 0.032), down-regulation of cfa-miR-505 (FC = 1.56, p = 0.016) in PS group which showed concentric cardiac hypertrophy, and down-regulation of cfa-miR-30c (FC = 3.45, p = 0.013 in MMVD group; FC = 3.31, p = 0.014 in PS group) and cfa-let-7b (FC = 11.42, p = 0.049 in MMVD group; FC = 5.88, p = 0.01 in PS group) in both MMVD and PS groups. In addition, the unsupervised hierarchical clustering of differentially expressed microRNAs in each group resulted in complete separation of healthy dogs from dogs with heart diseases. Therefore, eleven microRNAs among 291 microRNAs were identified as differentially expressed circulating microRNAs related to MMVD or PS in dogs. This pilot study demonstrates that the microRNAs identified in this study could be possible candidates for novel biomarker or therapeutic target related to cardiac hypertrophy in dogs.

Thrombin Regulation in Children Differs from Adults in the Absence and Presence of Heparin

1994 ◽  
Vol 72 (06) ◽  
pp. 836-842 ◽  
Author(s):  
Maureen Andrew ◽  
Lesley Mitchell ◽  
Patsy Vegh ◽  
Frederick Ofosu
Keyword(s):  
Thrombin Generation ◽  
Heparin Therapy ◽  
Thromboembolic Complications ◽  
Central Importance ◽  
Chromogenic Assay

SummaryThe physiologic mechanisms that protect children from thromboembolic complications are not known. We investigated the regulation of thrombin in children because of its central importance to thrombosis. The capacity to generate thrombin in vitro (chromogenic assay) was decreased by 26% in plasmas from children (1-16 yrs; n = 102) compared to adults ([20-45 yrs; n = 20; p <0.001]). The addition of purified prothrombin to plasmas from children increased thrombin generation to adult values. The capacity of plasmas to inhibit 125I-α-thrombin was increased by 21% in children compared to adults (p = 0.020), with significantly more thrombin complexed to a2-macroglobulin (α2M) in children.When DVT occur in children, adult guidelines for heparin therapy are used. At low heparin concentrations (0.1 and 0.2 U/ml), thrombin generation was decreased by 30% in children compared to adults (p <0.001). At high heparin levels (0.4 U/ml), thrombin generation was negligible in all plasmas. ATIII inhibited over 95% of thrombin in all plasmas in the presence of heparin.In summary, thrombin regulation differs in children from adults and may protect children from thromboembolic complications. When DVT do occur, heparin requirements may differ in children compared to adults.

scholarly journals Thrombin generation and thromboelastometry tests in dogs with chronic kidney disease

2020 ◽  
Vol 40 (2) ◽  
pp. 113-120
Author(s):  
Daniele S. Gonçalves ◽  
Silvano S. Geraldes ◽  
Rita Carolina F. Duarte ◽  
Maria das Graças Carvalho ◽  
Priscylla Tatiana C. Guimarães-Okamoto ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Viscoelastic Properties ◽  
Thrombin Generation ◽  
Control Group ◽  
Fibrinogen Concentration ◽  
Discrete State ◽  
Significance Level ◽  
Bleeding Episodes ◽  
Healthy Dogs

ABSTRACT: Patients with chronic kidney disease (CKD) have paradoxical hemostatic potential because they have bleeding episodes but are also prone to thrombosis. Few studies have evaluated blood viscoelastic properties in dogs with kidney disease; on the other hand, hypercoagulability has been observed in these patients. It is also emphasized that the platelet function and its participation in this process have not yet been fully understood. The objective of this study was to evaluate and compare the Thrombin Generation Test (TGT) and also viscoelastic properties of the blood measured by thromboelastometry (TEM) in dogs with proteinuria in CKD. Twenty healthy dogs (Control Group) and 19 dogs with CKD in stage III or IV, classified according to International Renal Interest Society - IRIS, were selected, and the reference test of urine protein:creatinine ratio (UPCR) should be greater than one (CKD group). Blood samples for TEM, thrombin generation, Prothrombin Time (PT), activated Partial Thromboplastin Time (aPTT), and fibrinogen concentration was collected at a single time for both groups after inclusion criteria being confirmed. Statistical analysis was performed according to the distribution of variables at 5% significance level. Differences were observed between healthy dogs and those with proteinuria in CKD noted in TEM. The TGT was unable to differentiate between sick and healthy groups. However, when the nephropathy was stratified, increases in TTP and peak thrombin concentration by TGT were observed in females and dogs over 30 days of diagnosis of CKD. Both tests signaled a discrete state of hypercoagulability. In fact, TEM is more sensitive to detect hypercoagulability in dogs with CKD. However, the TGT has potential clinical application by allowing long-term sample storage.

Relative Oral Bioavailability of Two Amoxicillin–Clavulanic Acid Formulations in Healthy Dogs: A Pilot Study

2019 ◽  
Vol 55 (1) ◽  
pp. 14-22 ◽  
Author(s):  
Jennifer Moczarnik ◽  
Darren J. Berger ◽  
James O. Noxon ◽  
Dana N. LeVine ◽  
Zhoumeng Lin ◽  
...  
Keyword(s):  
Pilot Study ◽  
Clavulanic Acid ◽  
Coefficient Of Variation ◽  
Oral Bioavailability ◽  
Drug Exposure ◽  
Comparative Investigation ◽  
Pharmacokinetic Parameters ◽  
Pilot Investigation ◽  
Amoxicillin Clavulanic Acid ◽  
Healthy Dogs

ABSTRACT The use of human generic amoxicillin–clavulanic acid formulations in veterinary medicine is currently lacking supportive evidence. This pilot study was conducted to determine preliminary pharmacokinetic parameters and relative oral bioavailability of a human generic and veterinary proprietary 4:1 amoxicillin–clavulanic acid formulation in healthy dogs to evaluate whether drug exposure was similar and to determine if further comparative investigation is warranted. Each dog received a single oral dose of each formulation containing 500:125 mg of amoxicillin–clavulanic acid at two separate instances with a 2 wk washout period between product administration. Following drug administration, blood was collected at fixed times over 24 hr to measure plasma amoxicillin and clavulanic acid concentrations using liquid chromatography–mass spectrometry. There were no statistically significant differences between pharmacokinetic parameters of either formulation. Clavulanic acid showed greater between-dog variation in drug exposure between formulations compared with amoxicillin and was also observed to be more variable within the veterinary proprietary formulation. The average relative oral bioavailability was 98.2% (23.6% coefficient of variation) for amoxicillin and 152.6% (64.3% coefficient of variation) for clavulanic acid between formulations. This pilot investigation supports the need for further bioequivalence studies regarding these formulations before commenting on product interchangeability.

scholarly journals Microbiologic Evaluation of Gallbladder Bile of Healthy Dogs and Dogs with Iatrogenic Hypercortisolism: A Pilot Study

2010 ◽  
Vol 24 (1) ◽  
pp. 224-228 ◽  
Author(s):  
P.H. Kook ◽  
S. Schellenberg ◽  
P. Grest ◽  
C.E. Reusch ◽  
L. Corboz ◽  
...  
Keyword(s):  
Pilot Study ◽  
Gallbladder Bile ◽  
Healthy Dogs
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