Reproducibility, stability, and biological variability of thrombin generation using calibrated automated thrombography in healthy dogs

2018 ◽  
Vol 47 (2) ◽  
pp. 218-226 ◽  
Author(s):  
Benoît Cuq ◽  
Shauna L. Blois ◽  
R. Darren Wood ◽  
Gabrielle Monteith ◽  
Anthony C. Abrams-Ogg ◽  
...  
Keyword(s):  
Thrombin Generation ◽  
Biological Variability ◽  
Healthy Dogs

Development and analytic validation of an immunoassay for the quantification of canine S100A12 in serum and fecal samples and its biological variability in serum from healthy dogs

2011 ◽  
Vol 144 (3-4) ◽  
pp. 200-209 ◽  
Author(s):  
Romy M. Heilmann ◽  
David J. Lanerie ◽  
Craig G. Ruaux ◽  
Niels Grützner ◽  
Jan S. Suchodolski ◽  
...  
Keyword(s):  
Biological Variability ◽  
Fecal Samples ◽  
Healthy Dogs

scholarly journals Thrombin generation and thromboelastometry tests in dogs with chronic kidney disease

2020 ◽  
Vol 40 (2) ◽  
pp. 113-120
Author(s):  
Daniele S. Gonçalves ◽  
Silvano S. Geraldes ◽  
Rita Carolina F. Duarte ◽  
Maria das Graças Carvalho ◽  
Priscylla Tatiana C. Guimarães-Okamoto ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Viscoelastic Properties ◽  
Thrombin Generation ◽  
Control Group ◽  
Fibrinogen Concentration ◽  
Discrete State ◽  
Significance Level ◽  
Bleeding Episodes ◽  
Healthy Dogs

ABSTRACT: Patients with chronic kidney disease (CKD) have paradoxical hemostatic potential because they have bleeding episodes but are also prone to thrombosis. Few studies have evaluated blood viscoelastic properties in dogs with kidney disease; on the other hand, hypercoagulability has been observed in these patients. It is also emphasized that the platelet function and its participation in this process have not yet been fully understood. The objective of this study was to evaluate and compare the Thrombin Generation Test (TGT) and also viscoelastic properties of the blood measured by thromboelastometry (TEM) in dogs with proteinuria in CKD. Twenty healthy dogs (Control Group) and 19 dogs with CKD in stage III or IV, classified according to International Renal Interest Society - IRIS, were selected, and the reference test of urine protein:creatinine ratio (UPCR) should be greater than one (CKD group). Blood samples for TEM, thrombin generation, Prothrombin Time (PT), activated Partial Thromboplastin Time (aPTT), and fibrinogen concentration was collected at a single time for both groups after inclusion criteria being confirmed. Statistical analysis was performed according to the distribution of variables at 5% significance level. Differences were observed between healthy dogs and those with proteinuria in CKD noted in TEM. The TGT was unable to differentiate between sick and healthy groups. However, when the nephropathy was stratified, increases in TTP and peak thrombin concentration by TGT were observed in females and dogs over 30 days of diagnosis of CKD. Both tests signaled a discrete state of hypercoagulability. In fact, TEM is more sensitive to detect hypercoagulability in dogs with CKD. However, the TGT has potential clinical application by allowing long-term sample storage.

Effect of dalteparin administration on thrombin generation kinetics in healthy dogs

2017 ◽  
Vol 46 (2) ◽  
pp. 269-277 ◽  
Author(s):  
Carolyn Gara-Boivin ◽  
Jérôme R.E. de Castillo ◽  
Marilyn E. Dunn ◽  
Christian Bédard
Keyword(s):  
Thrombin Generation ◽  
Healthy Dogs

Thrombin generation and inhibition in the mediation of ischemic neuronal death

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S19-S19
Author(s):  
Marlise de Castro Ribeiro ◽  
Jerome Badaut ◽  
Melanie Price ◽  
Marita Meins ◽  
Julien Bogousslavsky ◽  
...  
Keyword(s):  
Neuronal Death ◽  
Thrombin Generation

Thrombin generation in paediatric patients with congenital heart disease

2008 ◽  
Vol 28 (S 01) ◽  
pp. S61-S66 ◽  
Author(s):  
G. Cvirn ◽  
A. Rosenkranz ◽  
B. Leschnik ◽  
W. Raith ◽  
W. Muntean ◽  
...  
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Negative Correlation ◽  
Congenital Heart ◽  
Thrombin Generation ◽  
Significant Negative Correlation ◽  
Peak Height ◽  
Global Test ◽  
Paediatric Patients ◽  
Age Range

SummaryThrombin generation was studied in paediatric patients with congenital heart disease (CHD) undergoing cardiac surgery using the calibrated automated thrombography (CAT) in terms of the lag time until the onset of thrombin formation, time to thrombin peak maximum (TTP), endogenous thrombin potential (ETP), and thrombin peak height. The suitability to determine the coagulation status of these patients was investigated. Patients, material, methods: CAT data of 40 patients with CHD (age range from newborn to 18 years) were compared to data using standard coagulation parameters such as prothrombin (FII), antithrombin (AT), tissue factor pathway inhibitor (TFPI), prothrombin fragment 1.2 (F 1.2), thrombin-antithrombin (TAT), activated partial thromboplastin time (aPTT), and prothrombin time (PT). Results: A significant positive correlation was seen between ETP and FII (p < 0.01; r = 0.369), as well as between peak height and F II (p < 0.01; r = 0.483). A significant negative correlation was seen between ETP and TFPI values (p < 0.05; r = –0.225) while no significant correlation was seen between peak height and TFPI. A significant negative correlation was seen between F 1.2 generation and ETP (p < 0.05; r = –0.254) and between F 1.2 generation and peak height (p < 0.05; r = –0.236). No correlation was seen between AT and ETP or peak. Conclusions: CAT is a good global test reflecting procoagulatory and inhibitory factors of the haemostatic system in paediatric patients with CHD.

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