scholarly journals Comparison of effusion cell block and biopsy immunohistochemistry in mesothelial hyperplasia, mesothelioma, and carcinoma in dogs

2021 ◽  
Author(s):  
Elspeth M. Milne ◽  
Martina Piviani ◽  
Hannah M. Hodgkiss‐Geere ◽  
Chiara Piccinelli ◽  
Michael Cheeseman ◽  
...  
Keyword(s):  
Effusion Cell ◽  
Cell Block

scholarly journals A Retrospective Single-Institutional Analysis of the Usefulness of Pleural Effusion-Cell Block for Diagnosing Malignant Pleural Mesothelioma

Haigan ◽  
2020 ◽  
Vol 60 (7) ◽  
pp. 972-978
Author(s):  
Masaki Hashimoto ◽  
Michiko Yuki ◽  
Toru Nakamichi ◽  
Akifumi Nakamura ◽  
Ayumi Kuroda ◽  
...  
Keyword(s):  
Pleural Effusion ◽  
Malignant Pleural Mesothelioma ◽  
Institutional Analysis ◽  
Effusion Cell ◽  
Pleural Mesothelioma ◽  
Cell Block

scholarly journals Cytological Evaluation of Two Methods of Effusion Cell Block Preparations

2017 ◽  
Vol 4 (4) ◽  
pp. A403-A409
Author(s):  
DeepaSiddappa Masur ◽  
ShilpaSomashekhar Biradar
Keyword(s):  
Effusion Cell ◽  
Cell Block ◽  
Cytological Evaluation

Microstructural investigation of surface roughness in MBE-grown AlAs

1995 ◽  
Vol 53 ◽  
pp. 454-455
Author(s):  
R. Rajesh ◽  
R. Droopad ◽  
C. H. Kuo ◽  
R. W. Carpenter ◽  
G. N. Maracas
Keyword(s):  
Thin Film ◽  
Real Time ◽  
Growth Control ◽  
Native Oxide ◽  
Effusion Cell ◽  
Surface Oxide Layer ◽  
Microstructural Investigation ◽  
Mbe Growth ◽  
Film Substrate ◽  
And Control

Knowledge of material pseudodielectric functions at MBE growth temperatures is essential for achieving in-situ, real time growth control. This allows us to accurately monitor and control thicknesses of the layers during growth. Undesired effusion cell temperature fluctuations during growth can thus be compensated for in real-time by spectroscopic ellipsometry. The accuracy in determining pseudodielectric functions is increased if one does not require applying a structure model to correct for the presence of an unknown surface layer such as a native oxide. Performing these measurements in an MBE reactor on as-grown material gives us this advantage. Thus, a simple three phase model (vacuum/thin film/substrate) can be used to obtain thin film data without uncertainties arising from a surface oxide layer of unknown composition and temperature dependence.In this study, we obtain the pseudodielectric functions of MBE-grown AlAs from growth temperature (650°C) to room temperature (30°C). The profile of the wavelength-dependent function from the ellipsometry data indicated a rough surface after growth of 0.5 μm of AlAs at a substrate temperature of 600°C, which is typical for MBE-growth of GaAs.

Cell Block- an Useful Technique for Immunohistochemistry from FNAC Samples

2018 ◽  
Vol 2 (3) ◽  
pp. 10-15
Author(s):  
Niranjan Rout ◽  
◽  
Sashibhusan Dash ◽  
Janmejaya Mohapatra ◽  
Sagarika Samantaray ◽  
...  
Keyword(s):  
Cell Block

Cell Block and Its Impact in the Diagnosis of Jaw Lesions over Fine Needle Aspiration Cytology

2021 ◽  
pp. 1-7
Author(s):  
Kaveri Hallikeri ◽  
Biji Babu ◽  
Archana Sudhakaran ◽  
Roshni Monteiro
Keyword(s):  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
Dentigerous Cyst ◽  
Needle Aspiration ◽  
Histopathological Diagnosis ◽  
Cell Block ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Dentigerous Cysts ◽  
Needle Aspiration Cytology

<b><i>Objectives:</i></b> To determine the role and efficacy of fine needle aspiration cytology (FNAC) and cell block in diagnosis of jaw lesions and compare the agreement between FNAC and cell block to predict the diagnosis. <b><i>Method:</i></b> The sample comprised 51 cases, including 12 odontogenic keratocysts (OKCs), 8 ameloblastomas, 22 radicular cysts, 7 dentigerous cysts, and 1 each of intraosseous mucoepidermoid carcinoma (MEC) and adenomatoid odontogenic tumor (AOT). FNAC samples remaining after hematoxylin and eosin (H&amp;E)-stained cytosmear diagnosis were centrifuged at 3,000 rpm for 10 min. The supernatant was discarded and sediment mixed with 2–3 mL alcohol and filtered. To this, 10% formalin was added, filtered, taken for routine processing, and stained with H&amp;E. The result of FNAC smear and cell block was compared with histopathological diagnosis. <b><i>Results:</i></b> On cytological examination of the smears, 7 OKCs and 22 radicular cysts were diagnosed, whereas ameloblastomas, AOT, intraosseous MEC, and dentigerous cysts were not. This gave an agreement of 56.8% with the biopsy reports. Cell block sections stained with H&amp;E of 12 OKCs, 22 radicular cysts, 1 MEC, and 3 cases of ameloblastoma offered a diagnosis in accordance with the biopsies giving an agreement of 74.5%, while dentigerous cyst and AOT failed to do so. In comparison with FNAC, additionally 5 cases of OKC and 1 of MEC could be detected, and in ameloblastoma, out of 8 cases, only 3 yielded a concordant diagnosis through the cell block technique. <b><i>Conclusion:</i></b> In comparison with FNAC, the architectural pattern and the morphology of the cells were better preserved by the cell block technique. This substantiates that cell block could be used as an ancillary technique to aid in definitive diagnosis of head and neck swellings.

Validation of PD-L1 Immunohistochemical Testing on Formalin Fixed Paraffin Embedded (FFPE) Cell Block (CB) Preparations

2020 ◽  
Vol 9 (6) ◽  
pp. S53-S54
Author(s):  
Priyanka Karam ◽  
Yonah Ziemba ◽  
Sean Hacking ◽  
Karen Chau ◽  
Suganthi Soundararajan ◽  
...  
Keyword(s):  
Cell Block ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed ◽  
Immunohistochemical Testing

Cell Block Optimization: A Comparative Study of Quality Variables in 4 Different Cell Block Methods

2021 ◽  
pp. 1-7
Author(s):  
Marsali Newman ◽  
Matthew Walsh ◽  
Rosemary Jeffrey ◽  
Richard Hiscock
Keyword(s):  
Comparative Study ◽  
Processing Time ◽  
Morphological Features ◽  
Cell Block ◽  
Total Processing Time ◽  
Block Methods ◽  
Molecular Studies ◽  
Agar Method ◽  
Md Anderson ◽  
Ancillary Studies

<b><i>Objective:</i></b> The cell block (CB) is an important adjunct to cytological preparations in diagnostic cytopathology. Optimizing cellular material in the CB is essential to the success of ancillary studies such as immunohistochemistry (IHC) and molecular studies (MS). Our aim was to identify which CB method was most suitable in a variety of specimen types and levels of cellularity. <b><i>Study Design:</i></b> We assessed 4 different CB methods, thrombin clot method (TCM), MD Anderson method (MDAM), gelatin foam method (GFM), and agar method (AM), with descriptive observations and ranking of the methods based on quantity of cells and morphological features. <b><i>Results:</i></b> TCM performed best in ranking for both quantity of cells and morphological features, followed by MDAM, GFM, and AM. Lack of adjuvant in the MDAM resulted in some unique morphological advantages which, however, also resulted in inconsistent performance. In low cellularity cases insufficient cells were frequently identified on slides from MDAM and AM CBs. Technique touch time was similar for all methods, with total processing time being shortest for TCM followed by MDAM, GFM, and AM. <b><i>Conclusions:</i></b> TCM was the most robust CB technique, retaining high scores for ranking of quantity and morphology in a variety of specimen cellularities and specimen types.

Sign in / Sign up

Export Citation Format

Share Document