Analytical variability of estimated platelet counts on canine blood smears

2018 ◽  
Vol 47 (2) ◽  
pp. 197-204 ◽  
Author(s):  
Saverio Paltrinieri ◽  
Veronica Paciletti ◽  
Jari Zambarbieri
Keyword(s):  
Platelet Counts ◽  
Analytical Variability ◽  
Blood Smears

Estimation of white blood cell and platelet counts in ovine blood smears, and a comparison with the ADVIA 120 hematology analyzer

2020 ◽  
Vol 49 (2) ◽  
pp. 222-226
Author(s):  
Eleni G. Katsogiannou ◽  
Labrini V. Athanasiou ◽  
Panagiotis D. Katsoulos ◽  
Zoe S. Polizopoulou ◽  
Athanasia Tzivara ◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Platelet Counts ◽  
Blood Smears ◽  
Hematology Analyzer ◽  
Advia 120

scholarly journals Pseudothrombocytopenia: Manifestation of a New Type of Platelet Agglutinin

Blood ◽  
1973 ◽  
Vol 42 (4) ◽  
pp. 541-549 ◽  
Author(s):  
David P. Shreiner ◽  
William R. Bell
Keyword(s):  
Clinical Significance ◽  
Divalent Cation ◽  
Gamma Globulin ◽  
Platelet Rich Plasma ◽  
Platelet Counts ◽  
Normal Plasma ◽  
Blood Smears ◽  
New Type

Abstract Spuriously low electronic platelet counts were obtained on blood collected in EDTA from six patients. Platelet numbers on blood smears and electronic counts performed on blood collected in other anticoagulants were normal. Plasma or sera from these patients agglutinated homologous, as well as autologous platelets, in the presence of EDTA or DTPA, but agglutination did not occur in blood or platelet-rich plasma anticoagulated with heparin, oxalate, or citrate. Agglutination in the presence of EDTA was not inhibited by heparin, α-tocopherol, or N-ethylmaleimide, but was inhibitied by excess EDTA or divalent cation. The agglutinin was temperature independent in five of the six patients and had the physical and immunologic characteristics of a gamma globulin. The clinical significance and mechanism of EDTA-dependent agglutination of platelets is unknown.

scholarly journals Evaluation of EDTA induced pseudothrombocytopenia and the effect of alternative anticoagulants

2014 ◽  
Vol 4 (8) ◽  
pp. 626-629
Author(s):  
A Shrestha ◽  
S Karki
Keyword(s):  
Platelet Count ◽  
Peripheral Blood ◽  
Peripheral Blood Smear ◽  
Institute Of Medicine ◽  
Platelet Counts ◽  
Blood Smears ◽  
Low Platelet Count ◽  
The Mean ◽  
Correct Estimation ◽  
Peripheral Blood Smears

Background: Artifactual Thrombocytopenia is a condition in which there is falsely lowered platelet in patients who have thrombocytopenia but the absence of petechiae or echymoses. Pseudothrombocytopenia is also an artifactual thrombocytopenia caused by anticoagulant dependent agglutinins. The aim of this study was to compare the platelet count in pseudothrombocytopenia in EDTA anticoagulated samples and other alternative anticoagulants.Materials and methods: This study was performed in the department of hemotology hematology, Institute of medicine. All cases during study period were evaluated by EDTA-anticoagulated whole blood samples but criteria for selecting pseudothrombocytopenia patients was unexpectedly low platelet counts with clumping/aggregate on peripheral blood smear. Additional samples were collected in sodium citrate and heparin for examined.Results: A total of 50 patients aged between 18 to 90 years were found to have pseudothrombocytopenia. Platelet counts in samples anticoagulated with EDTA ranged from 20x109/l to 149x109/l and samples from same patients anticoagulated with citrate ranged from 41x109 /l to 312x109 /l and heparin showed platelet count ranging from 29x10 9 /l to 210x109 /l. The mean platelet count in EDTA- anticoagulated blood of individuals with pseudothrombocytopenia was 104x109/l whereas the mean platelet count in citrate and heparin-anticoagulated samples was 151x109/land123x109/l respectively. Platelet counts decreased dramatically in the EDTA samples in contrast to the samples anticoagulated with citrate or heparin post four hours of collection.Conclusion: Peripheral blood smears should be examined for platelet clumping/aggregates in cases with low platelet count not correlating with clinical presentation or in isolated thrombocytopenia flagged in hematology analyser. Alternative anticoagulants should be used for correct estimation of platelet count.DOI: http://dx.doi.org/10.3126/jpn.v4i8.11498 Journal of Pathology of Nepal; Vol.4,No. 8 (2014) 626-629

Estimation of Platelet Counts on Feline Blood Smears

1999 ◽  
Vol 28 (2) ◽  
pp. 42-45 ◽  
Author(s):  
Séverine Tasker ◽  
Peter J. Cripps ◽  
Andrew J. Mackin
Keyword(s):  
Platelet Counts ◽  
Blood Smears

Pseudothrombocytopenia Associated With Infectious Mononucleosis

2003 ◽  
Vol 127 (1) ◽  
pp. e17-e18 ◽  
Author(s):  
A. T. Hsieh ◽  
T. Y. Chao ◽  
Y. C. Chen
Keyword(s):  
Infectious Mononucleosis ◽  
Peripheral Blood ◽  
Ethylenediaminetetraacetic Acid ◽  
Severe Thrombocytopenia ◽  
Platelet Counts ◽  
Blood Smears ◽  
Peripheral Blood Smears ◽  
Possible Cause

Abstract A 22-year-old man was hospitalized for assessment of thrombocytopenia and fever. Examination showed that he had infectious mononucleosis and moderately severe thrombocytopenia that was asymptomatic. Examination of blood smears revealed that the thrombocytopenia was caused by the clumping of platelets. We made a diagnosis of ethylenediaminetetraacetic acid–dependent pseudothrombocytopenia after excluding other infectious mononucleosis–related mechanisms of thrombocytopenia. When the patient recovered from infectious mononucleosis 2 months later, his thrombocytopenia improved, and no platelet clumping in peripheral blood smears was noted. Ethylenediaminetetraacetic acid–dependent pseudothrombocytopenia should always be considered as a possible cause of reported low platelet counts, even in patients with infectious mononucleosis and splenomegaly.

Ultrastructural investigations of MDL 19,660-induced effects on the canine platelet and megakaryocyte

1990 ◽  
Vol 48 (3) ◽  
pp. 374-375
Author(s):  
D.E. Loudy ◽  
J. Sprinkle-Cavallo ◽  
J.T. Yarrington ◽  
F.Y. Thompson ◽  
J.P. Gibson
Keyword(s):  
Antidepressant Drug ◽  
Beagle Dogs ◽  
Long Term Effects ◽  
Short Term ◽  
Platelet Counts ◽  
Toxicological Studies ◽  
Induced Aggregation ◽  
Internal Architecture

Previous short term toxicological studies of one to two weeks duration have demonstrated that MDL 19,660 (5-(4-chlorophenyl)-2,4-dihydro-2,4-dimethyl-3Hl, 2,4-triazole-3-thione), an antidepressant drug, causes a dose-related thrombocytopenia in dogs. Platelet counts started to decline after two days of dosing with 30 mg/kg/day and continued to decrease to their lowest levels by 5-7 days. The loss in platelets was primarily of the small discoid subpopulation. In vitro studies have also indicated that MDL 19,660: does not spontaneously aggregate canine platelets and has moderate antiaggregating properties by inhibiting ADP-induced aggregation. The objectives of the present investigation of MDL 19,660 were to evaluate ultrastructurally long term effects on platelet internal architecture and changes in subpopulations of platelets and megakaryocytes.Nine male and nine female beagle dogs were divided equally into three groups and were administered orally 0, 15, or 30 mg/kg/day of MDL 19,660 for three months. Compared to a control platelet range of 353,000- 452,000/μl, a doserelated thrombocytopenia reached a maximum severity of an average of 135,000/μl for the 15 mg/kg/day dogs after two weeks and 81,000/μl for the 30 mg/kg/day dogs after one week.

Iron Deficiency Increases Platelet Counts in Rats

2009 ◽  
Vol 47 (05) ◽  
Author(s):  
R Evstatiev ◽  
A Bukaty ◽  
S Kulnigg ◽  
L Teischinger ◽  
C Gasche
Keyword(s):  
Iron Deficiency ◽  
Platelet Counts

Platelet Mobilization Induced by PAF and Its Role in the Thrombocytosis Triggered by Adrenaline in Rats

1989 ◽  
Vol 62 (04) ◽  
pp. 1107-1111 ◽  
Author(s):  
Hugo C Castro-Faria-Neto ◽  
Patricia T Bozza ◽  
Marco A Martins ◽  
Paulo M F L Dias ◽  
Patricia M R Silva ◽  
...  
Keyword(s):  
Receptor Antagonists ◽  
Blood Samples ◽  
Platelet Counts ◽  
Platelet Number ◽  
Edta Solution ◽  
Web 2086 ◽  
Dependent Mechanism

SummaryThe injection of PAP (6 μg/kg, i. v.) induced, in rats, haemoconcentration accompanied by an increase in the platelet number, as attested by the counts of platelets in blood samples diluted in formalin-free EDTA solution. This increase was significant at 15 min, peaked from 1 to 4 h and returned to basal levels 24 h after the lipid administration. The release of platelets induced by PAP was inhibited dose-dependently by specific PAP receptor antagonists such as WEB 2086 (0.5-2 mg/kg), BN 52021 and 48740 RP (5-25 mg/kg). Furthermore, platelet mobilization was clearly impaired in splenectomized animals stimulated by PAP, whereas thrombocytopenia and haemoconcentration by the same stimulus were intact. It was also noted that a second injection of PAP, 24 h after the initial stimulation with the lipid, failed to induce an increase in platelet counts, indicating autodesensitization. Desensitization to PAP or pretreatment with PAP antagonists clearly prevented the increase in the platelet counts after stimulation by adrenaline (15 μg/kg). These findings suggest that, in rats, PAP can induce release of platelets by a spleen-dependent mechanism and that this lipid may be relevant to the thrombocytosis triggered by adrenaline.

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