Estimation of white blood cell and platelet counts in ovine blood smears, and a comparison with the ADVIA 120 hematology analyzer

2020 ◽  
Vol 49 (2) ◽  
pp. 222-226
Author(s):  
Eleni G. Katsogiannou ◽  
Labrini V. Athanasiou ◽  
Panagiotis D. Katsoulos ◽  
Zoe S. Polizopoulou ◽  
Athanasia Tzivara ◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Platelet Counts ◽  
Blood Smears ◽  
Hematology Analyzer ◽  
Advia 120

Comparison of white blood cell counts by WNR, WDF, and WPC channels in Sysmex XN hematology analyzer

2015 ◽  
Vol 37 (6) ◽  
pp. 869-875 ◽  
Author(s):  
H. Kim ◽  
M. Hur ◽  
S.-G. Choi ◽  
K.-M. Oh ◽  
H.-W. Moon ◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Hematology Analyzer ◽  
White Blood Cell Counts

Performance of automated digital cell imaging analyzer Sysmex DI-60

2017 ◽  
Vol 56 (1) ◽  
Author(s):  
Hyeong Nyeon Kim ◽  
Mina Hur ◽  
Hanah Kim ◽  
Seung Wan Kim ◽  
Hee-Won Moon ◽  
...  
Keyword(s):  
Platelet Count ◽  
Blood Cell ◽  
White Blood Cell ◽  
Red Blood Cell ◽  
Cell Imaging ◽  
Platelet Counts ◽  
Manual Count ◽  
Manual Counting ◽  
Highly Correlated

AbstractBackground:The Sysmex DI-60 system (DI-60, Sysmex, Kobe, Japan) is a new automated digital cell imaging analyzer. We explored the performance of DI-60 in comparison with Sysmex XN analyzer (XN, Sysmex) and manual count.Methods:In a total of 276 samples (176 abnormal and 100 normal samples), white blood cell (WBC) differentials, red blood cell (RBC) classification and platelet (PLT) estimation by DI-60 were compared with the results by XN and/or manual count. RBC morphology between pre-classification and verification was compared according to the ICSH grading criteria. The manual count was performed according to the Clinical and Laboratory Standards Institute guidelines (H20-A2).Results:The overall concordance between DI-60 and manual count for WBCs was 86.0%. The agreement between DI-60 pre-classification and verification was excellent (weighted κ=0.963) for WBC five-part differentials. The correlation with manual count was very strong for neutrophils (r=0.955), lymphocytes (r=0.871), immature granulocytes (r=0.820), and blasts (r=0.879). RBC grading showed notable differences between DI-60 and manual counting on the basis of the ICSH grading criteria. Platelet count by DI-60 highly correlated with that by XN (r=0.945). However, DI-60 underestimated platelet counts in samples with marked thrombocytosis.Conclusions:The performance of DI-60 for WBC differential, RBC classification, and platelet estimation seems to be acceptable even in abnormal samples with improvement after verification. DI-60 would help optimize the workflow in hematology laboratory with reduced manual workload.

scholarly journals Effect of Trapping Method on Leukocyte Profiles of Black-Chested Spiny-Tailed Iguanas (Ctenosaura melanosterna): Implications for Zoologists in the Field

ISRN Zoology ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Andrew K. Davis ◽  
Leslie E. Ruyle ◽  
John C. Maerz
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Stress Hormones ◽  
Wild Animals ◽  
Direct Capture ◽  
Cell Counts ◽  
Blood Smears ◽  
Trapping Method ◽  
Effects Of Stress ◽  
Leukocyte Profiles

When wild animals are captured for zoological research, researchers must choose a method of capture, and often this can be some form of passive, baited cage trap, or a direct capture with nets or nooses. If information on basal levels of circulating leukocytes is a goal, these two methods may provide different information, since recent evidence indicates that animals that enter cage traps experience stress, and, elevated stress hormones are known to alter leukocyte numbers in circulation by lowering lymphocyte and raising heterophil numbers. We tested this idea using a study of Black-chested Spiny-tailed iguanas (Ctenosaura melanosterna), which were captured using cage traps () and noose (). Based on cell counts made from blood smears, iguanas caught with cage traps had significantly greater relative and absolute numbers of heterophils and higher heterophil-lymphocyte (H-L) ratios than those captured by noose. Cage-trapped animals also had a nonsignificant reduction in lymphocyte numbers. Similar trends were observed in animals captured with both methods. These patterns are consistent with the effects of stress hormones on white blood cell distributions and indicate that caution must be taken in interpreting leukocyte data from studies of wild animals captured with cage traps.

scholarly journals M2G1G2 white blood cell flag by three-part automated hematology analyzer: A hint to dengue infection in appropriate clinical context

2019 ◽  
Vol 11 (02) ◽  
pp. 103-106
Author(s):  
Maitrayee Roy ◽  
Akshay Bali
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Dengue Infection ◽  
Blood Film ◽  
Clinical Context ◽  
Normal Limits ◽  
Hematology Analyzer ◽  
Automated Hematology Analyzer

Abstract BACKGROUND: Automated hematology analyzers often generate many flags which can provide important clues to the underlying hematological abnormality. Although pathologists are generally well versed in recognizing the importance of flags indicating potential leukemic blasts, their utility in hinting toward infectious etiology, especially during epidemics, is less well known. We analyzed any abnormal flags generated by a three-part automated hematology analyzer in serologically confirmed cases of dengue. MATERIALS AND METHODS: The study included 28 patients diagnosed with serologically confirmed dengue infection. The venous samples were run on ABX Miros-60 three-part hematology analyzer. The complete blood count data and any abnormal flags were noted and correlated with peripheral blood film findings in all patients. RESULTS: An abnormal white blood cell (WBC) flag was noted in all 28 patients, including two in whom all other hematological parameters were within normal limits. In 26 (93%) patients, M2G1G2 WBC flag was noted while the samples of the remaining two patients generated M2G1 and L1M2G1G2 WBC flags, respectively. CONCLUSION: An abnormal WBC flag, when correlated in appropriate clinical context, especially during a dengue outbreak, can aid in targeting the cohort of patients who will require immediate referral for serological confirmation.

Evaluation of an Automated Digital Imaging System, Nextslide Digital Review Network, for Examination of Peripheral Blood Smears

2012 ◽  
Vol 136 (6) ◽  
pp. 660-667 ◽  
Author(s):  
Hongbo Yu ◽  
Chi Young Ok ◽  
Adam Hesse ◽  
Peter Nordell ◽  
Diane Connor ◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Digital Imaging ◽  
Peripheral Blood ◽  
Imaging System ◽  
Excellent Correlation ◽  
Nucleated Red Blood Cells ◽  
Blood Smears ◽  
Peripheral Blood Smears ◽  
Differential Counts

Context.—Several automated digital imaging systems have been introduced in recent years to improve turnaround time and proficiency in examining peripheral blood smears in hematology laboratories. Objective.—To evaluate a new automated digital imaging system, Nextslide Digital Review Network (Nextslide), for examination of peripheral blood smears. Design.—We evaluated 479 peripheral blood smears, of which 247 (51.6%) were included for comparison of Nextslide and manual white blood cell differential counts and morphology evaluation, 204 (42.6%) were included for comparison of Nextslide and CellaVision (DM96) differential counts, and 28 (5.8%) were neonatal samples examined for enumeration of nucleated red blood cells. Results.—Results from both method comparisons showed excellent correlation for all major white blood cell classes with correlation coefficients ranging from 0.70 to 0.99. Evaluation of white blood cell, red blood cell, and platelet morphology also showed good correlation among methods. White blood cell preclassification capability in the system was evaluated for rate and accuracy. Leukopenic samples demonstrated markedly decreased review time with Nextslide. Enumeration of nucleated red blood cells showed good correlation among methods. Conclusions.—Our evaluation of Nextslide shows excellent correlation when compared with conventional manual differentials and CellaVision (DM96) differentials for evaluation of peripheral blood smears.

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