Minor impact of patient alloantibodies against human platelet antigen ( HPA )‐15 in the effectiveness of platelet transfusion: A pilot study

Transfusion ◽  
2020 ◽  
Author(s):  
Hiroko Inoue ◽  
Rumi Sakamoto ◽  
Hiroko Nishimiya ◽  
Hiroe Sakamoto ◽  
Shota Terasu ◽  
...  
Keyword(s):  
Pilot Study ◽  
Platelet Transfusion ◽  
Human Platelet ◽  
Platelet Antigen ◽  
Human Platelet Antigen

Human Platelet Antigen 3 (HPA-3): Localization of the Determinant of the Alloantibody Leka (HPA-3a) to the C-Terminus of Platelet Glycoprotein IIb Heavy Chain and Contribution of O-Linked Carbohydrates

1993 ◽  
Vol 69 (05) ◽  
pp. 485-489 ◽  
Author(s):  
Isabelle Djaffar ◽  
Didier Vilette ◽  
Dominique Pidard ◽  
Jean-Luc Wautier ◽  
Jean-Philippe Rosa
Keyword(s):  
Amino Acids ◽  
Heavy Chain ◽  
Human Platelet ◽  
Platelet Glycoprotein ◽  
Fibrinogen Receptor ◽  
C Terminus ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
Determinant Structure ◽  
Polymerase Chain

SummaryThe human platelet antigen (HPA) 3 system is expressed on GPIIb, one subunit of GPIIb-IIIa, the platelet fibrinogen receptor. It was recently shown that HPA-3 was associated with an Ile843/Ser polymorphism. To investigate further HPA-3 determinant structure, we localized an HPA-3a determinant, recognized by the alloantiserum Leka, within the last 29 amino acids of GPIIbα. This region encompasses the polymorphic Ile843, which, as expected, is substituted into Ser in Leka-negative individuals, as shown by DNA sequence after polymerase chain reaction on platelet RNA. In addition, contribution of glycosylation to the determinant structure was demonstrated since the Leka antigenicity was strongly decreased after specifically removing nonterminal O-linked sugars, but not terminal sialic acids. We have thus refined the localization of an HPA-3a determinant within the last 29 amino acids, including Ile843, of GPIIb heavy chain, and shown that the Leka HPA-3a determinant is dependent, in part, upon the serine-linked carbohydrates adjacent to Ile/Ser843.

The Human Platelet Antigen HPA-la/lb (PIA1/PIA2)Polymorphism and Cerebral Ischaemia

1997 ◽  
Vol 78 (02) ◽  
pp. 964-965 ◽  
Author(s):  
Karl H Reuner ◽  
Michael Elgas ◽  
Michael Kaps ◽  
Andreas Ruf ◽  
Heinrich Patscheke
Keyword(s):  
Cerebral Ischaemia ◽  
Human Platelet ◽  
Platelet Antigen ◽  
Human Platelet Antigen

scholarly journals Human Platelet Antigen Genotyping and Expression of CD109 (Human Platelet Antigen 15) mRNA in Various Human Cell Types

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Sang Mee Hwang ◽  
Mi Jung Kim ◽  
Ho Eun Chang ◽  
Yun Ji Hong ◽  
Taek Soo Kim ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Human Platelet ◽  
Human Fibroblasts ◽  
Leukemia Cell ◽  
Embryonic Stem ◽  
Melting Curve ◽  
Leukemia Cell Line ◽  
Melting Curve Analysis ◽  
Platelet Antigen ◽  
Human Platelet Antigen

CD109 gene encodes a glycosylphosphatidylinositol-linked glycoprotein found in a subset of platelets and endothelial cell, and human platelet antigen (HPA) 15 is found on CD109. We evaluated the HPA genotype and/or the CD109 mRNA expression on two peripheral blood stem cells (PBSC), two peripheral bloods (PB), 12 granulocyte products, natural killer (NK)-92, B-lymphocyte (CO88BV59-1), K-562 leukemia cell line, human embryonic stem cell (hESC), and human fibroblasts (HF). HPA genotyping was performed by SNaPshot assay and CD109 mRNA expression was evaluated by real-time PCR with SYBR green and melting curve analysis. Genotype HPA-15a/-15a was found in PBSC#1 and two granulocyte products, and HPA-15a/-15b was found in PBSC#2, eight granulocyte products, NK-92, K-562, hESC, and HF, and HPA-15b/-15b was found in two granulocyte products. CD109 mRNA expression was highly increased in HF and increased in CD34+ and CD34− PBSCs and some granulocyte products, compared to the PB. However, the increase of expression level varied among the PBSC and granulocyte products. The CD109 mRNA expression of NK-92, K-562, hESC, and CO 88BV59-1 was not detected. HPA genotype was evaluated in various cells and the expression of CD109, which contains HPA 15, was different among cell lines and high in HF and PBSCs.

P008 Case report: Platelet refractory due to human platelet antigen-antibody, rather than human leukocyte antigen-antibodies

2019 ◽  
Vol 80 ◽  
pp. 59
Author(s):  
Justin Kreuter ◽  
Micah Zuccarelli ◽  
Patti Duellman ◽  
Laurie L. Wakefield ◽  
Manish J. Gandhi
Keyword(s):  
Case Report ◽  
Human Leukocyte Antigen ◽  
Human Platelet ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
Antigen Antibody
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