Response to Walker et al.: Residual bacterial detection rates after primary culture as determined by secondary culture and rapid testing

Transfusion ◽  
2020 ◽  
Vol 60 (10) ◽  
pp. 2460-2461
Author(s):  
Paul D. Mintz ◽  
Remo P. Vallejo
Keyword(s):  
Primary Culture ◽  
Rapid Testing ◽  
Bacterial Detection ◽  
Detection Rates ◽  
Secondary Culture

scholarly journals Effect of a natural plant ingredient — Pulsatilla  saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

2020 ◽  
Author(s):  
Liuhong Shen ◽  
Bolin Qian ◽  
Shangkui Lv ◽  
Liuchao You ◽  
Yue Zhang ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Interleukin 1 ◽  
Clinical Mastitis ◽  
Dose Effect ◽  
Bacterial Detection ◽  
Detection Rates ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Group D

Abstract Background To investigate the effect of Pulsatilla saponin B4, a purified extract form Pulsatilla Chinensis, on the treatment of clinical mastitis in dairy cows, 12 healthy cows were used as the control group (group A, no treatment), and 36 cows with mastitis were divided into 3 groups based on the quaque die (QD) intramuscular injection (IM) dose used in the as follows, group B (15 mL), group C (30 mL), group D (60 mL). Identified the microorganisms in milk during the experiment,samples in test groups were cultured, isolated, and verified by quantitative real-time polymerase chain reaction (qPCR). Somatic cell count (SCC) and serum inflammatory indices in cows were measured with a 1-day interval in group C and compared with group A. Results Results showed that bacterial detection rates were 100% (group B), 83.33% (group C) and 100% (group D). After the treatment bacterial detection rates dropped to 50.00% (group B), 50.00% (group C), and 41.67% (group D). The medicine was effective in all test groups and showed a dose-effect relationship. Serum haptoglobin (HP), prostaglandin E2 (PGE2), interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), interleukin-2 (IL-2), interleukin-8 (IL-8), interleukin-10 (IL-10) and tumour necrosis factor alpha (TNF-α), interleukin-4 (IL-4), leukotriene B4 (LTB4) also the SCC were significantly higher than healthy cows (P < 0.05) on the day (d) 1 in group C but had no significant difference with group A after treatment. Conclusion Treatment with Pulsatilla saponin B4 relieved mastitis, leading to relieve of bacterial infection, SCC and levels of serum inflammatory factors effectively in cows with mastitis after being administered for 4–6 days QD.

Impact of increasing sample volume from 4 ml to 8 ml on bacterial detection rates in apheresis platelets: a meta-analysis

Vox Sanguinis ◽  
2014 ◽  
Vol 108 (3) ◽  
pp. 318-320 ◽  
Author(s):  
R. Bruhn ◽  
B. Custer ◽  
S. Vanderpool ◽  
M. Townsend ◽  
H. Kamel ◽  
...  
Keyword(s):  
Meta Analysis ◽  
Sample Volume ◽  
Bacterial Detection ◽  
Detection Rates ◽  
Apheresis Platelets

scholarly journals Fusobacterium nucleatum and Bacteroides fragilis detection in colorectal tumours: Optimal target site and correlation with total bacterial load

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0262416
Author(s):  
Marie S. Rye ◽  
Kerryn L. Garrett ◽  
Robert A. Holt ◽  
Cameron F. Platell ◽  
Melanie J. McCoy
Keyword(s):  
Bacterial Species ◽  
Primary Tumour ◽  
Bacterial Load ◽  
Bacteroides Fragilis ◽  
Fusobacterium Nucleatum ◽  
Normal Epithelium ◽  
Bacterial Detection ◽  
Detection Rates ◽  
Metastatic Sites ◽  
Optimal Site

Background Mucosal infiltration by certain bacterial species may contribute to the development and progression of colorectal cancer (CRC). There is considerable variation in reported detection rates in human CRC samples and the extent to which bacterial infiltration varies across regions of the primary tumour is unknown. This study aimed to determine if there is an optimal site for bacterial detection within CRC tumours. Methods Presence of target bacterial species was assessed by quantitative real-time PCR (qPCR) in 42 human CRC tumours. Abundance in primary tumour regions, normal epithelium and at metastatic sites was investigated in an expanded cohort of 51 patients. Species presence/absence was confirmed by diversity profiling in five patients. Correlation with total bacterial load and clinicopathological features was assessed. Results Fusobacterium nucleatum and Bacteroides fragilis were detected in tumours from 43% and 24% of patients, respectively (17% positive for both species). The optimal detection site was the tumour luminal surface (TLS). Patients testing positive at the TLS frequently tested negative at other sites, including central tumour and invasive margin. F. nucleatum was detected at a higher frequency in tumour versus normal epithelium (p < 0.01) and was associated with more advanced disease (p = 0.01). Detection of both species correlated with total bacterial load. However, corroboration of qPCR results via diversity profiling suggests detection of these species may indicate a specific microbial signature. Conclusions This study supports a role for F. nucleatum in CRC development. Presence of F. nucleatum and B. fragilis varies across primary tumour regions, with the TLS representing the optimal site for bacterial detection.

Primary culture of rat ependymal cells in serum-free defined medium

1986 ◽  
Vol 390 (2) ◽  
pp. 199-209 ◽  
Author(s):  
M WEIBEL ◽  
B PETTMANN ◽  
J ARTAULT ◽  
M SENSENBRENNER ◽  
G LABOURDETTE
Keyword(s):  
Primary Culture ◽  
Defined Medium ◽  
Ependymal Cells ◽  
Serum Free

Implementation of an HIV Rapid Testing/Linkage to Care Project Among Homeless Individuals

2014 ◽  
Author(s):  
Henry Anaya ◽  
Herschel Knapp ◽  
Magdalena Esquivel ◽  
Sophia F. Rumanes ◽  
Jaimi N. Butler ◽  
...  
Keyword(s):  
Linkage To Care ◽  
Rapid Testing ◽  
Hiv Rapid Testing ◽  
Care Project

Predicting Fetal Chromosome Anomalies in the First Trimester Using Pregnancy Associated Plasma Protein-A: A Comparison of Statistical Methods

1993 ◽  
Vol 32 (02) ◽  
pp. 175-179 ◽  
Author(s):  
B. Brambati ◽  
T. Chard ◽  
J. G. Grudzinskas ◽  
M. C. M. Macintosh
Keyword(s):  
Logistic Regression ◽  
General Population ◽  
Likelihood Ratio ◽  
False Positive ◽  
False Positive Rate ◽  
Ratio Method ◽  
Detection Rates ◽  
Gaussian Distributions ◽  
Positive Rate ◽  
Likelihood Ratio Method

Abstract:The analysis of the clinical efficiency of a biochemical parameter in the prediction of chromosome anomalies is described, using a database of 475 cases including 30 abnormalities. A comparison was made of two different approaches to the statistical analysis: the use of Gaussian frequency distributions and likelihood ratios, and logistic regression. Both methods computed that for a 5% false-positive rate approximately 60% of anomalies are detected on the basis of maternal age and serum PAPP-A. The logistic regression analysis is appropriate where the outcome variable (chromosome anomaly) is binary and the detection rates refer to the original data only. The likelihood ratio method is used to predict the outcome in the general population. The latter method depends on the data or some transformation of the data fitting a known frequency distribution (Gaussian in this case). The precision of the predicted detection rates is limited by the small sample of abnormals (30 cases). Varying the means and standard deviations (to the limits of their 95% confidence intervals) of the fitted log Gaussian distributions resulted in a detection rate varying between 42% and 79% for a 5% false-positive rate. Thus, although the likelihood ratio method is potentially the better method in determining the usefulness of a test in the general population, larger numbers of abnormal cases are required to stabilise the means and standard deviations of the fitted log Gaussian distributions.

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