scholarly journals Zika virus RNA polymerase chain reaction on the utility channel of a commercial nucleic acid testing system

Transfusion ◽  
2018 ◽  
Vol 58 (3) ◽  
pp. 641-648 ◽  
Author(s):  
Mohamed Boujnan ◽  
Ashley J. Duits ◽  
Marco H.G.M. Koppelman
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Rna Polymerase ◽  
Zika Virus ◽  
Testing System ◽  
Nucleic Acid Testing ◽  
Chain Reaction ◽  
Virus Rna ◽  
Polymerase Chain

scholarly journals Nucleic Acid Testing of SARS-CoV-2

2021 ◽  
Vol 22 (11) ◽  
pp. 6150
Author(s):  
Hee-Min Yoo ◽  
Il-Hwan Kim ◽  
Seil Kim
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Reverse Transcription ◽  
Diagnostic Methods ◽  
Detection Methods ◽  
Molecular Diagnostic ◽  
Nucleic Acid Testing ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. The polymerase chain reaction (PCR)-based method including reverse transcription-polymerase chain reaction (RT-PCR) is the most widely used assay for the detection of SARS-CoV-2 RNA. Along with the RT-PCR method, digital PCR has emerged as a powerful tool to quantify nucleic acid of the virus with high accuracy and sensitivity. Non-PCR based techniques such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription recombinase polymerase amplification (RT-RPA) are considered to be rapid and simple nucleic acid detection methods and were reviewed in this paper. Non-conventional molecular diagnostic methods including next-generation sequencing (NGS), CRISPR-based assays and nanotechnology are improving the accuracy and sensitivity of COVID-19 diagnosis. In this review, we also focus on standardization of SARS-CoV-2 nucleic acid testing and the activity of the National Metrology Institutes (NMIs) and highlight resources such as reference materials (RM) that provide the values of specified properties. Finally, we summarize the useful resources for convenient COVID-19 molecular diagnostics.

scholarly journals Diagnostic Performance of Zika Virus Ribonucleic Acid (RNA) Polymerase Chain Reaction (PCR) in Urine Samples

2016 ◽  
Vol 3 (suppl_1) ◽  
Author(s):  
Loubiela Joseph ◽  
Samantha Manuel ◽  
Chris Roggeveen ◽  
Liane Virginia-Cova ◽  
Radjin Steingrover
Keyword(s):  
Polymerase Chain Reaction ◽  
Rna Polymerase ◽  
Zika Virus ◽  
Ribonucleic Acid ◽  
Diagnostic Performance ◽  
Urine Samples ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Optimization and Validation of a Real Time Reverse Transcriptase Polymerase Chain Reaction with RNA Internal Control to Detect Rubella RNA

2013 ◽  
Vol 5 (3) ◽  
pp. 185
Author(s):  
Winny Xie ◽  
Yusmiati Yusmiati
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Amniotic Fluid ◽  
Internal Control ◽  
Chain Reaction ◽  
Rubella Infection ◽  
Synthetic Dna ◽  
Congenital Rubella ◽  
Virus Rna ◽  
Polymerase Chain

BACKGROUND: According to a report from WHO, cases of rubella infection in Indonesia has increased up to 10-fold from 2007 to 2011. Despite no data of congenital rubella syndrome in the report, there are approximately 45,000 cases of babies born with heart failure and 0.1-0.3% live births with congenital deafness in Indonesia. Allegedly, rubella infection during pregnancy may play a role in this condition. This study aimed to optimize and validate a real-time reverse transcriptase polymerase chain reaction (RT-qPCR) method to detect rubella virus RNA as an aid for the diagnosis of congenital rubella infection.METHODS: Method optimization was conducted using nucleic acids extracted from Trimovax Merieux vaccine with the High Pure Viral Nucleic Acid Kit. One step RT-qPCR was performed with Quantifast Multiplex RTPCR+R Kit. Target synthetic DNA was designed and used to determine the sensitivity of the method. RNA internal control was synthesized to control the process of extraction and amplification.RESULTS: The analytical sensitivity of this method was as low as 5 copies target synthetic DNA/μl. The mean Coefficient of Variation (CV) % of the critical threshold (Ct) obtained were 2.71%, 1.20%, 1.62%, and 1.59% for within run, between run, between kit lots, and between operators, respectively. Recovery of the target synthetic DNA from amniotic fluid was 100.51% (by the log copies/μl) at the concentration of 1,000,000 copies/μl.CONCLUSION: RT-qPCR is successfully used for the detection of rubella virus RNA in vaccine and synthetic nucleic acid. With its high sensitivity, good precision and recovery, this method offers a means to improve the diagnosis of congenital rubella infection in developing countries like Indonesia.KEYWORDS: congenital rubella, RT-qPCR, prenatal diagnosis, amniotic fluid

scholarly journals Prolonged Detection of Zika Virus Nucleic Acid Among Symptomatic Pregnant Women: A Cohort Study

2018 ◽  
Vol 67 (4) ◽  
pp. 624-627 ◽  
Author(s):  
Matthew J Lozier ◽  
Eli S Rosenberg ◽  
Kate Doyle ◽  
Laura Adams ◽  
Liore Klein ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Puerto Rico ◽  
Pregnant Women ◽  
Zika Virus ◽  
Definitive Diagnosis ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Virus Rna ◽  
Polymerase Chain ◽  
Diagnosis Of Infection

Abstract A prospective cohort of women with reverse transcription polymerase chain reaction (RT-PCR) confirmed Zika virus infection aged 18–39 years in Puerto Rico found that pregnant women have about a 3-fold longer estimated median detection of Zika virus RNA in serum, which can increase definitive diagnosis of infection and facilitate timely and appropriate clinical management.

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