scholarly journals Predicting storage-dependent damage to red blood cells using nitrite oxidation kinetics, peroxiredoxin-2 oxidation, and hemoglobin and free heme measurements

Transfusion ◽  
2015 ◽  
Vol 55 (12) ◽  
pp. 2967-2978 ◽  
Author(s):  
Joo-Yeun Oh ◽  
Ryan Stapley ◽  
Victoria Harper ◽  
Marisa B. Marques ◽  
Rakesh P. Patel
Keyword(s):  
Red Blood Cells ◽  
Oxidation Kinetics ◽  
Blood Cells ◽  
Nitrite Oxidation ◽  
Peroxiredoxin 2 ◽  
Free Heme

scholarly journals Oxidation of Erythrocytes Enhance the Production of Reactive Species in the Presence of Artemisinins

2020 ◽  
Vol 21 (13) ◽  
pp. 4799 ◽  
Author(s):  
Ioannis Tsamesidis ◽  
Pierre Pério ◽  
Antonella Pantaleo ◽  
Karine Reybier
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Activation Process ◽  
Oxygen Species ◽  
Paramagnetic Resonance ◽  
Reactive Iron ◽  
Redox Activation ◽  
Reducing Conditions ◽  
Free Heme ◽  
Electron Paramagnetic

In red blood cells, hemoglobin iron represents the most plausible candidate to catalyze artemisinin activation but the limited reactivity of iron bound to hemoglobin does not play in favor for its direct involvement. Denatured hemoglobin appears a more likely candidate for artemisinin redox activation because it is expected to contain reactive iron and it has been described to release free heme and/or iron in erythrocyte. The aim of our study is to investigate, using three different methods: fluorescence, electron paramagnetic resonance and liquid chromatography coupled to mass spectrometry, how increasing the level of accessible iron into the red blood cells can enhance the reactive oxygen species (ROS) production derived from artemisinin. The over-increase of iron was achieved using phenylhydrazine, a strong oxidant that causes oxidative stress within erythrocytes, resulting in oxidation of oxyhemoglobin and leading to the formation of methemoglobin, which is subsequently converted into irreversible hemichromes (iron (III) compounds). Our findings confirmed, using the iron III chelator, desferrioxamine, the indirect participation of iron (III) compounds in the activation process of artemisinins. Furthermore, in strong reducing conditions, the activation of artemisinin and the consequent production of ROS was enhanced. In conclusion, we demonstrate, through the measurement of intra-erythrocytic superoxide and hydrogen peroxide production using various methods, that artemisinin activation can be drastically enhanced by pre-oxidation of erythrocytes.

Structural and functional analysis of native peroxiredoxin 2 in human red blood cells

2012 ◽  
Vol 44 (7) ◽  
pp. 1072-1077 ◽  
Author(s):  
Yuki Ogasawara ◽  
Takuya Ohminato ◽  
Yusuke Nakamura ◽  
Kazuyuki Ishii
Keyword(s):  
Functional Analysis ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Peroxiredoxin 2 ◽  
Human Red Blood Cells

Peroxiredoxin-2 as a candidate biomarker to test oxidative stress levels of stored red blood cells under blood bank conditions

Transfusion ◽  
2011 ◽  
Vol 51 (7) ◽  
pp. 1439-1449 ◽  
Author(s):  
Sara Rinalducci ◽  
Gian M. D'Amici ◽  
Barbara Blasi ◽  
Stefania Vaglio ◽  
Giuliano Grazzini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Bank ◽  
Peroxiredoxin 2 ◽  
Stress Levels

scholarly journals Irreversible hyperoxidation of peroxiredoxin 2 is caused by tert- butyl hydroperoxide in human red blood cells

FEBS Open Bio ◽  
2014 ◽  
Vol 4 (1) ◽  
pp. 848-852 ◽  
Author(s):  
Y.I. Ishida ◽  
M. Takikawa ◽  
T. Suzuki ◽  
M. Nagahama ◽  
Y. Ogasawara
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Peroxiredoxin 2 ◽  
Human Red Blood Cells ◽  
Tert Butyl Hydroperoxide

The hydroperoxide-induced oxidation kinetics of hemoglobin and parinaric acid in red blood cells

1990 ◽  
Vol 9 ◽  
pp. 184
Author(s):  
Jeroen J.M. van den Berg ◽  
Jos A.F. Op den Kamp ◽  
Ben Roelofsen ◽  
Bertram H. Lubin ◽  
Frans A. Kuypers
Keyword(s):  
Red Blood Cells ◽  
Oxidation Kinetics ◽  
Blood Cells ◽  
Parinaric Acid ◽  
Kinetics Of

Differential oxidation processes of peroxiredoxin 2 dependent on the reaction with several peroxides in human red blood cells

2019 ◽  
Vol 518 (4) ◽  
pp. 685-690
Author(s):  
Yo-ichi Ishida ◽  
Yuko Ichinowatari ◽  
Shoichi Nishimoto ◽  
Shin Koike ◽  
Kazuyuki Ishii ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Oxidation Processes ◽  
Peroxiredoxin 2 ◽  
Human Red Blood Cells

Accumulation of oxidized peroxiredoxin 2 in red blood cells and its prevention

Transfusion ◽  
2015 ◽  
Vol 55 (8) ◽  
pp. 1909-1918 ◽  
Author(s):  
Simone B. Bayer ◽  
Mark B. Hampton ◽  
Christine C. Winterbourn
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Peroxiredoxin 2
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