scholarly journals The Arabidopsis transcription factor AINTEGUMENTA orchestrates patterning genes and auxin signaling in the establishment of floral growth and form

2020 ◽  
Vol 103 (2) ◽  
pp. 752-768 ◽  
Author(s):  
Beth A. Krizek ◽  
Ivory C. Blakley ◽  
Yen‐Yi Ho ◽  
Nowlan Freese ◽  
Ann E. Loraine
Keyword(s):  
Transcription Factor ◽  
Auxin Signaling ◽  
Patterning Genes

The Drosophila dCREB-A gene is required for dorsal/ventral patterning of the larval cuticle

Development ◽  
1997 ◽  
Vol 124 (1) ◽  
pp. 181-193 ◽  
Author(s):  
D.J. Andrew ◽  
A. Baig ◽  
P. Bhanot ◽  
S.M. Smolik ◽  
K.D. Henderson
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Signaling Cascades ◽  
Loss Of Function ◽  
Larval Cuticle ◽  
Extracellular Signals ◽  
Patterning Genes ◽  
Lateral Structures ◽  
Cuticular Structures

We report on the characterization of the first loss-of-function mutation in a Drosophila CREB gene, dCREB-A. In the epidermis, dCREB-A is required for patterning cuticular structures on both dorsal and ventral surfaces since dCREB-A mutant larvae have only lateral structures around the entire circumference of each segment. Based on results from epistasis tests with known dorsal/ventral patterning genes, we propose that dCREB-A encodes a transcription factor that functions near the end of both the DPP- and SPI-signaling cascades to translate the corresponding extracellular signals into changes in gene expression. The lateralizing phenotype of dCREB-A mutants reveals a much broader function for CREB proteins than previously thought.

scholarly journals Genome-wide RNA-seq analysis indicates that the DAG1 transcription factor promotes hypocotyl elongation acting on ABA, ethylene and auxin signaling

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Riccardo Lorrai ◽  
Francesco Gandolfi ◽  
Alessandra Boccaccini ◽  
Veronica Ruta ◽  
Marco Possenti ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Hypocotyl Elongation ◽  
Auxin Signaling ◽  
Rna Seq ◽  
Genome Wide

scholarly journals A Novel AP2-Type Transcription Factor, SMALL ORGAN SIZE1, Controls Organ Size Downstream of an Auxin Signaling Pathway

2014 ◽  
Vol 55 (5) ◽  
pp. 897-912 ◽  
Author(s):  
Koichiro Aya ◽  
Tokunori Hobo ◽  
Kanna Sato-Izawa ◽  
Miyako Ueguchi-Tanaka ◽  
Hidemi Kitano ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Signaling Pathway ◽  
Organ Size ◽  
Auxin Signaling ◽  
Auxin Signaling Pathway

A basic/helix–loop–helix transcription factor controls leaf shape by regulating auxin signaling in apple

2020 ◽  
Vol 228 (6) ◽  
pp. 1897-1913
Author(s):  
Da‐Gang Hu ◽  
Nian Wang ◽  
Dong‐Hui Wang ◽  
Lailiang Cheng ◽  
Yan‐Xiu Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Leaf Shape ◽  
Auxin Signaling ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

scholarly journals An improved auxin-inducible degron system preserves native protein levels and enables rapid and specific protein depletion

2019 ◽  
Author(s):  
Kizhakke Mattada Sathyan ◽  
Brian D. McKenna ◽  
Warren D. Anderson ◽  
Fabiana M. Duarte ◽  
Leighton Core ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Protein Function ◽  
Specific Protein ◽  
Auxin Signaling ◽  
Auxin Response ◽  
Protein Levels ◽  
Genome Wide ◽  
Pb1 Domain ◽  
Inducible Protein ◽  
Endogenous Loci

Rapid perturbation of protein function permits the ability to define primary molecular responses while avoiding down-stream cumulative effects of protein dysregulation. The auxin-inducible degron (AID) system was developed as a tool to achieve rapid and inducible protein degradation in non-plant systems. However, tagging proteins at their endogenous loci results in chronic, auxin-independent degradation by the proteasome. To correct this deficiency, we expressed the Auxin Response Transcription Factor (ARF) in an improved inducible degron system. ARF is absent from previously engineered AID systems, but ARF is a critical component of native auxin signaling. In plants, ARF directly interacts with AID in the absence of auxin and we found that expression of the ARF Phox and Bem1 (PB1) domain suppresses constitutive degradation of AID-tagged proteins. Moreover, the rate of auxin-induced AID degradation is substantially faster in the ARF-AID system. To test the ARF-AID system in a quantitative and sensitive manner, we measured genome-wide changes in nascent transcription after rapidly depleting the ZNF143 transcription factor. Transciptional profiling indicates that ZNF143 activates transcription in cis and ZNF143 regulates promoter-proximal paused RNA Polymerase density. Rapidly inducible degradation systems that preserve the target protein’s native expression levels and patterns will revolutionize the study of biological systems by enabling specific and temporally defined protein dysregulation.

EIN3-Mediated Ethylene Signaling Attenuates Auxin Response during Hypocotyl Thermomorphogenesis

2021 ◽  
Author(s):  
Jae Young Kim ◽  
Young-Joon Park ◽  
June-Hee Lee ◽  
Zee Hwan Kim ◽  
Chung-Mo Park
Keyword(s):  
Transcription Factor ◽  
Cell Elongation ◽  
Fine Tuning ◽  
Auxin Signaling ◽  
Plant Responses ◽  
Proton Pumps ◽  
Natural Environments ◽  
Auxin Response ◽  
Hypocotyl Growth ◽  
Gene Encoding

Abstract The gaseous phytohormone ethylene plays vital roles in diverse developmental and environmental adaptation processes, such as fruit ripening, seedling establishment, mechanical stress tolerance, and submergence escape. It is also known that in the light, ethylene promotes hypocotyl growth by stimulating the expression of PHYTOCHROME INTERACTING FACTOR3 (PIF3) transcription factor, which triggers microtubule reorganization during hypocotyl cell elongation. In particular, ethylene has been implicated in plant responses to warm temperatures in recent years. However, it is currently unclear how ethylene signals are functionally associated with hypocotyl thermomorphogenesis at the molecular level. Here, we show that ETHYLENE-INSENSITIVE3 (EIN3)-mediated ethylene signals attenuate hypocotyl thermomorphogenesis by suppressing auxin response. At warm temperatures, when the activity of the PIF4 thermomorphogenesis promoter is prominently high, the ethylene-activated EIN3 transcription factor directly induces the transcription of ARABIDOPSIS PP2C CLADE D7 (APD7) gene encoding a protein phosphatase that inactivates the plasma membrane (PM) H+-ATPase proton pumps. In conjunction with the promotive role of the PM H+-ATPases in hypocotyl cell elongation, our observations strongly support that the EIN3-directed induction of APD7 gene is linked with the suppression of auxin-induced cell expansion, leading to the reduction of thermomorphogenic hypocotyl growth. Our data demonstrate that APD7 acts as a molecular hub that integrates ethylene and auxin signals into hypocotyl thermomorphogenesis. We propose that the ethylene-auxin signaling crosstalks via the EIN3-APD7 module facilitate the fine-tuning of hypocotyl thermomorphogenesis under natural environments, which often fluctuate in a complex manner.

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