scholarly journals A pepper RING‐type E3 ligase, CaASRF1, plays a positive role in drought tolerance via modulation of CaAIBZ1 stability

2019 ◽  
Vol 98 (1) ◽  
pp. 5-18 ◽  
Author(s):  
Hyunhee Joo ◽  
Chae Woo Lim ◽  
Sung Chul Lee
Keyword(s):  
Drought Tolerance ◽  
E3 Ligase ◽  
Positive Role ◽  
Ring Type

Zinc-finger protein MdBBX7/MdCOL9, a target of MdMIEL1 E3 ligase, confers drought tolerance in apple

2021 ◽  
Author(s):  
Pengxiang Chen ◽  
Fang Zhi ◽  
Xuewei Li ◽  
Wenyun Shen ◽  
Mingjia Yan ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
E3 Ligase ◽  
26S Proteasome ◽  
Negative Regulator ◽  
Binding Motif ◽  
Ethylene Response Factor ◽  
Finger Protein

Abstract Water deficit is one of the main challenges for apple (Malus × domestica) growth and productivity. Breeding drought-tolerant cultivars depends on a thorough understanding of the drought responses of apple trees. Here, we identified the zinc-finger protein B-BOX 7/CONSTANS-LIKE 9 (MdBBX7/MdCOL9), which plays a positive role in apple drought tolerance. The overexpression of MdBBX7 enhanced drought tolerance, whereas knocking down MdBBX7 expression reduced it. Chromatin immunoprecipitation-sequencing (ChIP-seq) analysis identified one cis-element of MdBBX7, CCTTG, as well as its known binding motif, the T/G box. ChIP-seq and RNA-seq identified 1,197 direct targets of MdBBX7, including ETHYLENE RESPONSE FACTOR (ERF1), EARLY RESPONSIVE TO DEHYDRATION 15 (ERD15), and GOLDEN2-LIKE 1 (GLK1) and these were further verified by ChIP-qPCR and electronic mobility shift assays. Yeast two-hybrid screen identified an interacting protein of MdBBX7, RING-type E3 ligase MYB30-INTERACTING E3 LIGASE 1 (MIEL1). Further examination revealed that MdMIEL1 could mediate the ubiquitination and degradation of MdBBX7 by the 26S proteasome pathway. Genetic interaction analysis suggested that MdMIEL1 acts as an upstream factor of MdBBX7. In addition, MdMIEL1 was a negative regulator of the apple drought stress response. Taken together, our results illustrate the molecular mechanisms by which the MdMIEL1–MdBBX7 module influences the response of apple to drought stress.

The RanBP2 SUMO E3 ligase is neither HECT- nor RING-type

2004 ◽  
Vol 11 (10) ◽  
pp. 984-991 ◽  
Author(s):  
Andrea Pichler ◽  
Puck Knipscheer ◽  
Hisato Saitoh ◽  
Titia K Sixma ◽  
Frauke Melchior
Keyword(s):  
E3 Ligase ◽  
Ring Type ◽  
Sumo E3 Ligase

scholarly journals A Novel WRKY Transcription Factor from Ipomoea trifida, ItfWRKY70, Confers Drought Tolerance in Sweet Potato

2022 ◽  
Vol 23 (2) ◽  
pp. 686
Author(s):  
Sifan Sun ◽  
Xu Li ◽  
Shaopei Gao ◽  
Nan Nie ◽  
Huan Zhang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Sweet Potato ◽  
Stress Responses ◽  
Leaf Tissue ◽  
Stomatal Aperture ◽  
Ros Scavenging ◽  
Ipomoea Trifida ◽  
Positive Role

WRKY transcription factors are one of the important families in plants, and have important roles in plant growth, abiotic stress responses, and defense regulation. In this study, we isolated a WRKY gene, ItfWRKY70, from the wild relative of sweet potato Ipomoea trifida (H.B.K.) G. Don. This gene was highly expressed in leaf tissue and strongly induced by 20% PEG6000 and 100 μM abscisic acid (ABA). Subcellar localization analyses indicated that ItfWRKY70 was localized in the nucleus. Overexpression of ItfWRKY70 significantly increased drought tolerance in transgenic sweet potato plants. The content of ABA and proline, and the activity of SOD and POD were significantly increased, whereas the content of malondialdehyde (MDA) and H2O2 were decreased in transgenic plants under drought stress. Overexpression of ItfWRKY70 up-regulated the genes involved in ABA biosynthesis, stress-response, ROS-scavenging system, and stomatal aperture in transgenic plants under drought stress. Taken together, these results demonstrated that ItfWRKY70 plays a positive role in drought tolerance by accumulating the content of ABA, regulating stomatal aperture and activating the ROS scavenging system in sweet potato.

scholarly journals An Arabidopsis E3 ligase HUB2 increases histone H2B monoubiquitination and enhances drought tolerance in transgenic cotton

2018 ◽  
Vol 17 (3) ◽  
pp. 556-568 ◽  
Author(s):  
Hong Chen ◽  
Hao Feng ◽  
Xueyan Zhang ◽  
Chaojun Zhang ◽  
Tao Wang ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
E3 Ligase ◽  
Transgenic Cotton ◽  
Histone H2b

scholarly journals A RING-type E3 ligase controls anther dehiscence by activating the jasmonate biosynthetic pathway geneDEFECTIVE IN ANTHER DEHISCENCE1in Arabidopsis

2013 ◽  
Vol 74 (2) ◽  
pp. 310-327 ◽  
Author(s):  
Yan-Jhu Peng ◽  
Ching-Fang Shih ◽  
Jun-Yi Yang ◽  
Choon-Meng Tan ◽  
Wei-Han Hsu ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
E3 Ligase ◽  
Anther Dehiscence ◽  
Ring Type
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