scholarly journals Hydroxyproline O ‐arabinosyltransferase mutants oppositely alter tip growth in Arabidopsis thaliana and Physcomitrella patens

2015 ◽  
Vol 85 (2) ◽  
pp. 193-208 ◽  
Author(s):  
Cora A. MacAlister ◽  
Carlos Ortiz‐Ramírez ◽  
Jörg D. Becker ◽  
José A. Feijó ◽  
Zachary B. Lippman
Keyword(s):  
Arabidopsis Thaliana ◽  
Tip Growth ◽  
Physcomitrella Patens

Microtubules and microfilaments in tip growth: evidence that microtubules impose polarity on protonemal growth in Physcomitrella patens

1988 ◽  
Vol 89 (4) ◽  
pp. 533-540 ◽  
Author(s):  
J. H. DOONAN ◽  
D. J. COVE ◽  
C. W. LLOYD
Keyword(s):  
Tip Growth ◽  
Physcomitrella Patens ◽  
Cytochalasin D ◽  
Immunofluorescence Study ◽  
Apical Dome ◽  
High Concentrations ◽  
Cytoskeletal Elements ◽  
Drug Studies ◽  
Tip Cells ◽  
Cytoskeletal Drugs

In this study we compare the contributions of Factin and microtubules to tip growth in filamentous cells of the moss Physcomitrella patens. In tip growth, expansion seems to be restricted to the hemispherical apical dome. Cytoskeletal elements have been suspected, from drug studies, to be involved in this but electron microscopy has generally not confirmed the presence of an apical cytoskeleton. However, in a previous immunofluorescence study we reported that microtubules could be seen to focus upon the apical dome in tip cells of the moss P. patens. In the present investigation F-actin has also been detected at the apices of these cells. Anti-cytoskeletal drugs were therefore used to differentiate between the roles of actin filaments and microtubules in tip growth. At high concentrations (30μM), the herbicide cremart de-polymerized microtubules and caused tip swelling. F-actin was still present under such conditions but its fragmentation by cytochalasin D suppressed this herbicide-induced swelling. On its own, cytochalasin D arrested tip growth without causing swollen tips. At lower concentrations, cremart disorganized microtubules rather than causing their complete depolymerization. Under these conditions, new but swollen growing points were initiated along the filament. The addition of taxol to cremart-treated filaments tended to reduce swelling and to re-polarize outgrowth. With particular combinations of these drugs, multiple lateral out-growths were initiated in the vicinity of the nucleus. It is concluded: (1) that F-actin is present at the tips of Physcomitrella caulonemal apical cells; (2) that unfragmented F-actin is necessary for outgrowth; (3) that even disorganized microtubules permit some degree of outgrowth but that an unperturbed distribution of axial microtubules, focussing upon an apex, is essential in order to impose tubular shape and directionality upon expansion.

scholarly journals Systematic survey of the function of ROP regulators and effectors during tip growth in the moss Physcomitrella patens

2018 ◽  
Vol 70 (2) ◽  
pp. 447-457 ◽  
Author(s):  
Carlisle Bascom ◽  
Graham M Burkart ◽  
Darren R Mallett ◽  
Jacquelyn E O’Sullivan ◽  
Alexis J Tomaszewski ◽  
...  
Keyword(s):  
Tip Growth ◽  
Physcomitrella Patens ◽  
Systematic Survey

scholarly journals VOZ; Isolation and Characterization of Novel Vascular Plant Transcription Factors with a One-Zinc Finger from Arabidopsis thaliana

2004 ◽  
Vol 45 (7) ◽  
pp. 845-854 ◽  
Author(s):  
Nobutaka Mitsuda ◽  
Toru Hisabori ◽  
Kunio Takeyasu ◽  
Masa H. Sato
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Dna Binding ◽  
Physcomitrella Patens ◽  
Cultured Cells ◽  
Vascular Plant ◽  
Amino Acid Sequence Similarity ◽  
Cis Acting ◽  
Isolation And Characterization

Abstract A 38-bp pollen-specific cis-acting region of the AVP1 gene is involved in the expression of the Arabidopsis thaliana V-PPase during pollen development. Here, we report the isolation and structural characterization of AtVOZ1 and AtVOZ2, novel transcription factors that bind to the 38-bp cis-acting region of A. thaliana V-PPase gene, AVP1. AtVOZ1 and AtVOZ2 show 53% amino acid sequence similarity. Homologs of AtVOZ1 and AtVOZ2 are found in various vascular plants as well as a moss, Physcomitrella patens. Promoter-β-glucuronidase reporter analysis shows that AtVOZ1 is specifically expressed in the phloem tissue and AtVOZ2 is strongly expressed in the root. In vivo transient effector-reporter analysis in A. thaliana suspension-cultured cells demonstrates that AtVOZ1 and AtVOZ2 function as transcriptional activators in the Arabidopsis cell. Two conserved regions termed Domain-A and Domain-B were identified from an alignment of AtVOZ proteins and their homologs of O. sativa and P. patens. AtVOZ2 binds as a dimer to the specific palindromic sequence, GCGTNx7ACGC, with Domain-B, which is comprised of a functional novel zinc coordinating motif and a conserved basic region. Domain-B is shown to function as both the DNA-binding and the dimerization domains of AtVOZ2. From highly the conservative nature among all identified VOZ proteins, we conclude that Domain-B is responsible for the DNA binding and dimerization of all VOZ-family proteins and designate it as the VOZ-domain.

scholarly journals Structural Sterols Are Involved in Both the Initiation and Tip Growth of Root Hairs in Arabidopsis thaliana

2010 ◽  
Vol 22 (9) ◽  
pp. 2999-3019 ◽  
Author(s):  
Miroslav Ovečka ◽  
Tobias Berson ◽  
Martina Beck ◽  
Jan Derksen ◽  
Jozef Šamaj ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Tip Growth ◽  
Root Hairs

scholarly journals Physcomitrella patens: a model to investigate the role of RAC/ROP GTPase signalling in tip growth

2010 ◽  
Vol 61 (7) ◽  
pp. 1917-1937 ◽  
Author(s):  
D. Magnus Eklund ◽  
Emma M. Svensson ◽  
Benedikt Kost
Keyword(s):  
Tip Growth ◽  
Physcomitrella Patens ◽  
Rop Gtpase

scholarly journals Myosin XI Is Essential for Tip Growth in Physcomitrella patens

2010 ◽  
Vol 22 (6) ◽  
pp. 1868-1882 ◽  
Author(s):  
Luis Vidali ◽  
Graham M. Burkart ◽  
Robert C. Augustine ◽  
Erin Kerdavid ◽  
Erkan Tüzel ◽  
...  
Keyword(s):  
Tip Growth ◽  
Physcomitrella Patens ◽  
Myosin Xi

scholarly journals Pollen Tube and Root-Hair Tip Growth Is Disrupted in a Mutant of Arabidopsis thaliana

1993 ◽  
Vol 103 (3) ◽  
pp. 979-985 ◽  
Author(s):  
J. Schiefelbein ◽  
M. Galway ◽  
J. Masucci ◽  
S. Ford
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Root Hair ◽  
Tip Growth

scholarly journals Differential Requirements for RAD51 in Physcomitrella patens and Arabidopsis thaliana Development and DNA Damage Repair

2007 ◽  
Vol 19 (10) ◽  
pp. 3080-3089 ◽  
Author(s):  
Ulrich Markmann-Mulisch ◽  
Edelgard Wendeler ◽  
Oliver Zobell ◽  
Gabriele Schween ◽  
Hans-Henning Steinbiss ◽  
...  
Keyword(s):  
Dna Damage ◽  
Arabidopsis Thaliana ◽  
Physcomitrella Patens ◽  
Dna Damage Repair ◽  
Damage Repair
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