scholarly journals New initiatives atThe Plant Journalto better support the plant science community

2012 ◽  
Vol 72 (2) ◽  
pp. 173-174
Author(s):  
Christoph Benning
Keyword(s):  
Plant Science ◽  
Science Community

scholarly journals A modular cloning toolkit for genome editing in plants

2019 ◽  
Author(s):  
Florian Hahn ◽  
Andrey Korolev ◽  
Laura Sanjurjo Loures ◽  
Vladimir Nekrasov
Keyword(s):  
Genome Editing ◽  
Higher Order ◽  
Plant Science ◽  
Golden Gate ◽  
Coding Sequences ◽  
Guide Rna ◽  
Guide Rnas ◽  
Science Community ◽  
Modular Cloning ◽  
Multiple Expression

AbstractBackgroundCRISPR/Cas has recently become a widely used genome editing tool in various organisms, including plants. Applying CRISPR/Cas often requires delivering multiple expression units into plant and hence there is a need for a quick and easy cloning procedure. The modular cloning (MoClo), based on the Golden Gate (GG) method, has enabled development of cloning systems with standardised genetic parts, e.g. promoters, coding sequences or terminators, that can be easily interchanged and assembled into expression units, which in their own turn can be further assembled into higher order multigene constructs.ResultsHere we present an expanded cloning toolkit that contains ninety-nine modules encoding a variety of CRISPR/Cas-based nucleases and their corresponding guide RNA backbones. Among other components, the toolkit includes a number of promoters that allow expression of CRISPR/Cas nucleases (or any other coding sequences) and their guide RNAs in monocots and dicots. As part of the toolkit, we present a set of modules that enable quick and facile assembly of tRNA-sgRNA polycistronic units without a PCR step involved. We also demonstrate that our tRNA-sgRNA system is functional in wheat protoplasts.ConclusionsWe believe the presented CRISPR/Cas toolkit is a great resource that will contribute towards wider adoption of the CRISPR/Cas genome editing technology and modular cloning by researchers across the plant science community.

scholarly journals Building a pathway for diversity in plant sciences in Argentina: highlighting the work of women scientists through virtual activities

2020 ◽  
Author(s):  
Gabriela Alejandra Auge ◽  
María José de Leone ◽  
Rocío Deanna ◽  
Sonia Oliferuk ◽  
Pamela Anahí Ribone ◽  
...  
Keyword(s):  
Plant Science ◽  
Extension Programs ◽  
Diverse Range ◽  
Diverse Workforce ◽  
The Social ◽  
Wide Range ◽  
Scientific Poster ◽  
Science Community ◽  
Plant Sciences ◽  
Experimental Approaches

AbstractEncouraging the participation of a diverse workforce in academia increases plurality as it broadens the range of skills, ways of thinking and experiences. Institutions and professional societies have been putting efforts on building plans that help make workplaces, conferences, education and extension programs more relatable to a highly diverse population. Argentina has an overall gender-balanced workforce in the sciences (~53% women/total), with an even higher representation in disciplines related to plant sciences. However, media outlets and national conferences related to genetics, botany, plant physiology, ecology and molecular biology, fail to reflect those numbers as the proportion of women invited for interviews, plenary lectures, and symposia falls below ~30%. As a way to increase the visibility of the wealth of plant science topics and experimental approaches in which Argentinian women work, and to facilitate connections among them across the country and abroad, we created the Argentinian Women in Plant Science network (https://argplantwomen.weebly.com/). This group has grown to over 200 members, representing a wide range of career stages and research topics. Since April, and taking advantage of the confinement situation, our weekly webinar series highlighting women plant scientists has reached an average audience of 60-70 participants, with a record of 100. Recently, we have begun a series of open professional development webinars to reach a wider public. Our first webinar, focused on Scientific poster design, had ~250 participants, most of them undergrad and graduate students from all over the country covering a diverse range of disciplines, including the social sciences. Even though we have immersed ourselves in the plant science community with our weekly seminars, we have expanded our goals with activities aimed to reach out to a much wider audience with webinars and teacher training workshops, hopefully making plant science more attainable to all.

New Phytologist- serving the plant science community

2003 ◽  
Vol 161 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Ian Woodward ◽  
Jonathan Ingram
Keyword(s):  
Plant Science ◽  
Science Community

scholarly journals How to build an effective research network: lessons from two decades of the GARNet plant science community

2020 ◽  
Vol 71 (22) ◽  
pp. 6881-6889
Author(s):  
Geraint Parry ◽  
Yoselin Benitez-Alfonso ◽  
Daniel J Gibbs ◽  
Murray Grant ◽  
Andrea Harper ◽  
...  
Keyword(s):  
Collaborative Research ◽  
Science Research ◽  
Research Network ◽  
Plant Science ◽  
Future Research ◽  
Practical Implementation ◽  
Science Community ◽  
Experimental Approaches ◽  
The Uk ◽  
Significant Effort

Abstract Successful collaborative research is dependent on excellent ideas and innovative experimental approaches, as well as the provision of appropriate support networks. Collaboration requires venues, infrastructures, training facilities, and, perhaps most importantly, a sustained commitment to work together as a community. These activities do not occur without significant effort, yet can be facilitated and overseen by the leadership of a research network that has a clearly defined role to help build resources for their community. Over the past 20 years, this is a role that the UKRI-BBSRC-funded GARNet network has played in the support of the UK curiosity-driven, discovery-led plant science research community. This article reviews the lessons learnt by GARNet in the hope that they can inform the practical implementation of current and future research networks.

Blueprints for green biotech: development and application of standards for plant synthetic biology

2016 ◽  
Vol 44 (3) ◽  
pp. 702-708 ◽  
Author(s):  
Nicola J. Patron
Keyword(s):  
Synthetic Biology ◽  
Dna Synthesis ◽  
Genome Editing ◽  
Dna Sequences ◽  
Large Scale ◽  
De Novo ◽  
Plant Science ◽  
Custom Made ◽  
Science Community ◽  
Plant Synthetic Biology

Synthetic biology aims to apply engineering principles to the design and modification of biological systems and to the construction of biological parts and devices. The ability to programme cells by providing new instructions written in DNA is a foundational technology of the field. Large-scale de novo DNA synthesis has accelerated synthetic biology by offering custom-made molecules at ever decreasing costs. However, for large fragments and for experiments in which libraries of DNA sequences are assembled in different combinations, assembly in the laboratory is still desirable. Biological assembly standards allow DNA parts, even those from multiple laboratories and experiments, to be assembled together using the same reagents and protocols. The adoption of such standards for plant synthetic biology has been cohesive for the plant science community, facilitating the application of genome editing technologies to plant systems and streamlining progress in large-scale, multi-laboratory bioengineering projects.

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