scholarly journals Development of multiplex real‐time PCR assays for differential detection of capripoxvirus, parapoxvirus and foot‐and‐mouth disease virus

2021 ◽  
Author(s):  
Amaresh Das ◽  
Yin Wang ◽  
Shawn Babiuk ◽  
Jianfa Bai ◽  
Kimberly Dodd ◽  
...  
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Real Time Pcr ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Differential Detection ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Pcr Assays

Molecular epidemiology and phylogenetic analysis of foot and mouth disease virus type ‘O’ and evaluation of its carrier state in cattle of Assam by Real time PCR

2018 ◽  
Author(s):  
Sangeeta Baro ◽  
Krishna Sharma ◽  
Biswajyoti Sharma ◽  
Shantanu Tamuly ◽  
P. Deka ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Real Time ◽  
Disease Virus ◽  
Real Time Pcr ◽  
Sandwich Elisa ◽  
Foot And Mouth Disease ◽  
Carrier State ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

The molecular epidemiological study of foot-and-mouth disease virus (FMDV) has been carried out from different outbreaks in Assam the present study is based on the nucleotide sequencingof circulating FMDV serotype. The samples were subjected to sandwich ELISA, multiplex-PCR and molecular phylogeny to identify the type species. The phylogenetic analysis of virus sequence revealed similarity with theBangladesh isolates in the major branching pattern. The serotype ‘O’has found to be dominant and responsible for most of the recentoutbreaks.Thepersistence of serotype ‘O’ and cytokines expression of IL-1á, IL-1â, IFN-á, TNF-á in blood of recovered animals were done by Real time PCR. The findings indicated that IL-1á, IFN-á and TNF-á genes were up-regulated upto 3 months post infection but IL-1â found to be down regulated with progression of recovery. The present study thus supports that real-time PCR is a powerful technique for reliable detection of persistent FMDV in recovered animals.

scholarly journals Direct detection and characterization of foot-and-mouth disease virus in East Africa using a field-ready real-time PCR platform

2017 ◽  
Vol 65 (1) ◽  
pp. 221-231 ◽  
Author(s):  
E. L. A. Howson ◽  
B. Armson ◽  
N. A. Lyons ◽  
E. Chepkwony ◽  
C. J. Kasanga ◽  
...  
Keyword(s):  
Real Time ◽  
East Africa ◽  
Disease Virus ◽  
Real Time Pcr ◽  
Direct Detection ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Development and evaluation of tailored specific real-time RT-PCR assays for detection of foot-and-mouth disease virus serotypes circulating in East Africa

2016 ◽  
Vol 237 ◽  
pp. 114-120 ◽  
Author(s):  
Katarzyna Bachanek-Bankowska ◽  
Herieth R. Mero ◽  
Jemma Wadsworth ◽  
Valerie Mioulet ◽  
Raphael Sallu ◽  
...  
Keyword(s):  
Real Time ◽  
East Africa ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Pcr Assays

scholarly journals GoPrime: Development of an In Silico Framework to Predict the Performance of Real-Time PCR Primers and Probes Using Foot-and-Mouth Disease Virus as a Model

Pathogens ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 303
Author(s):  
Emma L A Howson ◽  
Richard J Orton ◽  
Valerie Mioulet ◽  
Tiziana Lembo ◽  
Donald P King ◽  
...  
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Real Time Pcr ◽  
In Silico ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Sequence Variability ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
The Impact

Real-time PCR (rPCR) is a widely accepted diagnostic tool for the detection and quantification of nucleic acid targets. In order for these assays to achieve high sensitivity and specificity, primer and probe-template complementarity is essential; however, mismatches are often unavoidable and can result in false-negative results and errors in quantifying target sequences. Primer and probe sequences therefore require continual evaluation to ensure they remain fit for purpose. This paper describes the development of a linear model and associated computational tool (GoPrime) designed to predict the performance of rPCR primers and probes across multiple sequence data. Empirical data were generated using DNA oligonucleotides (n = 90) that systematically introduced variation in the primer and probe target regions of a diagnostic assay routinely used to detect foot-and-mouth disease virus (FMDV); an animal virus that exhibits a high degree of sequence variability. These assays revealed consistent impacts of patterns of substitutions in primer and probe-sites on rPCR cycle threshold (CT) and limit of detection (LOD). These data were used to populate GoPrime, which was subsequently used to predict rPCR results for DNA templates (n = 7) representing the natural sequence variability within FMDV. GoPrime was also applicable to other areas of the FMDV genome, with predictions for the likely targets of a FMDV-typing assay consistent with published experimental data. Although further work is required to improve these tools, including assessing the impact of primer-template mismatches in the reverse transcription step and the broader impact of mismatches for other assays, these data support the use of mathematical models for rapidly predicting the performance of rPCR primers and probes in silico.

scholarly journals Utility of automated real-time RT-PCR for the detection of foot-and-mouth disease virus excreted in milk

2006 ◽  
Vol 37 (1) ◽  
pp. 121-132 ◽  
Author(s):  
Scott M. Reid ◽  
Satya Parida ◽  
Donald P. King ◽  
Geoffrey H. Hutchings ◽  
Andrew E. Shaw ◽  
...  
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Laboratory validation of two real-time RT-PCR methods with 5′-tailed primers for an enhanced detection of foot-and-mouth disease virus

2017 ◽  
Vol 246 ◽  
pp. 90-94 ◽  
Author(s):  
Frank Vandenbussche ◽  
David J. Lefebvre ◽  
Ilse De Leeuw ◽  
Steven Van Borm ◽  
Kris De Clercq
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth
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