Development and evaluation of multiplex real‐time RT‐PCR assays for the detection and differentiation of foot‐and‐mouth disease virus and Seneca Valley virus 1

2019 ◽  
Vol 67 (2) ◽  
pp. 604-616 ◽  
Author(s):  
Yin Wang ◽  
Amaresh Das ◽  
Wanglong Zheng ◽  
Elizabeth Porter ◽  
Lizhe Xu ◽  
...  
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Seneca Valley Virus ◽  
Pcr Assays

scholarly journals Development and evaluation of tailored specific real-time RT-PCR assays for detection of foot-and-mouth disease virus serotypes circulating in East Africa

2016 ◽  
Vol 237 ◽  
pp. 114-120 ◽  
Author(s):  
Katarzyna Bachanek-Bankowska ◽  
Herieth R. Mero ◽  
Jemma Wadsworth ◽  
Valerie Mioulet ◽  
Raphael Sallu ◽  
...  
Keyword(s):  
Real Time ◽  
East Africa ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Pcr Assays

scholarly journals Utility of automated real-time RT-PCR for the detection of foot-and-mouth disease virus excreted in milk

2006 ◽  
Vol 37 (1) ◽  
pp. 121-132 ◽  
Author(s):  
Scott M. Reid ◽  
Satya Parida ◽  
Donald P. King ◽  
Geoffrey H. Hutchings ◽  
Andrew E. Shaw ◽  
...  
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Laboratory validation of two real-time RT-PCR methods with 5′-tailed primers for an enhanced detection of foot-and-mouth disease virus

2017 ◽  
Vol 246 ◽  
pp. 90-94 ◽  
Author(s):  
Frank Vandenbussche ◽  
David J. Lefebvre ◽  
Ilse De Leeuw ◽  
Steven Van Borm ◽  
Kris De Clercq
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Foot And Mouth

One-step SYBR green-based real-time RT-PCR assay for detection of foot-and-mouth disease virus circulating in India

Virus Genes ◽  
2022 ◽  
Author(s):  
Jitendra K. Biswal ◽  
Biswa Ranjan Jena ◽  
Syed Zeeshan Ali ◽  
Rajeev Ranjan ◽  
Jajati K. Mohapatra ◽  
...  
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Sybr Green ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
One Step

scholarly journals Combining Multiple Assays Improves Detection and Serotyping of Foot-and-Mouth Disease Virus. A Practical Example with Field Samples from East Africa

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1583
Author(s):  
Efrem Foglia ◽  
Tiziana Lembo ◽  
Rudovick Kazwala ◽  
Divine Ekwem ◽  
Gabriel Shirima ◽  
...  
Keyword(s):  
Real Time ◽  
East Africa ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Mouth Disease Virus ◽  
Field Samples ◽  
Foot And Mouth ◽  
Pcr Targeting

Multiple serotypes and topotypes of foot-and-mouth disease virus (FMDV) circulate in endemic areas, posing considerable impacts locally. In addition, introductions into new areas are of great concern. Indeed, in recent years, multiple FMDV outbreaks, caused by topotypes that have escaped from their original areas, have been recorded in various parts of the world. In both cases, rapid and accurate diagnosis, including the identification of the serotype and topotype causing the given outbreaks, plays an important role in the implementation of the most effective and appropriate measures to control the spread of the disease. In the present study, we describe the performance of a range of diagnostic and typing tools for FMDV on a panel of vesicular samples collected in northern Tanzania (East Africa, EA) during 2012–2018. Specifically, we tested these samples with a real-time RT-PCR targeting 3D sequence for pan-FMDV detection; an FMDV monoclonal antibody-based antigen (Ag) detection and serotyping ELISA kit; virus isolation (VI) on LFBKαVβ6 cell line; and a panel of four topotype-specific real-time RT-PCRs, specifically tailored for circulating strains in EA. The 3D real-time RT-PCR showed the highest diagnostic sensitivity, but it lacked typing capacity. Ag-ELISA detected and typed FMDV in 71% of sample homogenates, while VI combined with Ag-ELISA for typing showed an efficiency of 82%. The panel of topotype-specific real-time RT-PCRs identified and typed FMDV in 93% of samples. However, the SAT1 real-time RT-PCR had the highest (20%) failure rate. Briefly, topotype-specific real-time RT-PCRs had the highest serotyping capacity for EA FMDVs, although four assays were required, while the Ag-ELISA, which was less sensitive, was the most user-friendly, hence suitable for any laboratory level. In conclusion, when the four compared tests were used in combination, both the diagnostic and serotyping performances approached 100%.

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