First detection of bovine papillomavirus type 2 in cutaneous wart lesions from ovines

2018 ◽  
Vol 65 (4) ◽  
pp. 939-943 ◽  
Author(s):  
J. Mazzuchelli-de-Souza ◽  
R. F. de Carvalho ◽  
D. G. Módolo ◽  
C. E. Thompson ◽  
R. P. Araldi ◽  
...  
Keyword(s):  
Bovine Papillomavirus ◽  
Cutaneous Wart

Bovine Papillomavirus Type-2 (BPV-2) Infection and Expression of Uroplakin IIIb, a Novel Urothelial Cell Marker, in Urinary Bladder Tumors of Cows

2005 ◽  
Vol 42 (6) ◽  
pp. 812-818 ◽  
Author(s):  
S. Roperto ◽  
V. Ambrosio ◽  
G. Borzacchiello ◽  
P. Galati ◽  
O. Paciello ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Single Cells ◽  
Urothelial Cell ◽  
Biological Behavior ◽  
Bovine Papillomavirus ◽  
Immunohistochemical Markers ◽  
Cell Layers ◽  
Urothelial Tumors ◽  
Differentiation Pattern

Several immunohistochemical markers have been used to define the differentiation pattern of urothelial cell tumors of the urinary bladder. We investigated the expression of the recently characterized uroplakin (UP) IIIb, an urothelium-specific and differentiation-dependent protein, in 39 urothelial tumors of the urinary bladder in cows that had suffered from chronic enzootic hematuria for several years. Bovine papillomavirus type 2 DNA was amplified and UP IIIb protein was detected in all these tumors. In papillomas and papillary carcinomas, UP IIIb expression was mostly seen as superficial staining; luminal and peripheral patterns were also observed. In nonpapillary carcinomas, UP IIIb appeared to define clearly the cell membrane lining intercellular and intracellular lumina as well as the cell borders in deeper cell layers. In benign and malignant lesions, an intracytoplasmic immunoreactivity was also detected. Coarse intracytoplasmic UP IIIb-positive material close to the nucleus occurred in some malignant cells. Focally strong membraneous immunostaining that marked single cells with complete ringlike peripheral pattern was seen. Although UP IIIb expression does not seem to correlate with the biological behavior of urothelial tumors, it appears to be a highly sensitive marker for bovine urothelial tumors.

scholarly journals Bovine papillomavirus type 2 infects the urinary bladder of water buffalo (Bubalus bubalis) and plays a crucial role in bubaline urothelial carcinogenesis

2013 ◽  
Vol 94 (2) ◽  
pp. 403-408 ◽  
Author(s):  
Sante Roperto ◽  
Valeria Russo ◽  
Ayhan Ozkul ◽  
Aylin Sepici-Dincel ◽  
Paola Maiolino ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Cell Transformation ◽  
Bubalus Bubalis ◽  
Immunofluorescence Assay ◽  
Receptor Interaction ◽  
Bovine Papillomavirus ◽  
Clinically Normal ◽  
Bladder Carcinogenesis ◽  
Β Receptor

Bovine papillomavirus type 2 (BPV-2) has been shown to infect and play a role in urinary bladder carcinogenesis of buffaloes grazed on pastures with ferns from the Marmara and Black Sea Regions of Turkey. BPV-2 DNA has been found in both neoplastic and non-neoplastic lesions of the urinary bladder. Furthermore, this virus may be a normal inhabitant of the urinary bladder since BPV-2 DNA has also been detected in clinically normal buffaloes. The viral activation by fern immunosuppressant or carcinogen may trigger the urothelial cell transformation. The E5 oncoprotein was solely detected in urothelial tumours and appeared to be co-localized with the overexpressed and phosphorylated platelet derived growth factor (PDGF) β receptor in a double-colour immunofluorescence assay. Our results indicate that the E5–PDGF β receptor interaction also occurs in spontaneous tumours of the bubaline urinary bladder, revealing an additional role of BPV-2 in bladder carcinogenesis of buffaloes.

scholarly journals Productive Infection of Bovine Papillomavirus Type 2 in the Urothelial Cells of Naturally Occurring Urinary Bladder Tumors in Cattle and Water Buffaloes

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62227 ◽  
Author(s):  
Sante Roperto ◽  
Valeria Russo ◽  
Ayhan Ozkul ◽  
Annunziata Corteggio ◽  
Aylin Sepici-Dincel ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Productive Infection ◽  
Bovine Papillomavirus ◽  
Urothelial Cells ◽  
Bladder Tumors ◽  
Water Buffaloes ◽  
Naturally Occurring

scholarly journals Evidence from a Series of 104 Equine Sarcoids Suggests That Most Sarcoids in New Zealand Are Caused by Bovine Papillomavirus Type 2, although Both BPV1 and BPV2 DNA Are Detectable in around 10% of Sarcoids

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3093
Author(s):  
John S. Munday ◽  
Geoff Orbell ◽  
Rob A. Fairley ◽  
Michael Hardcastle ◽  
Bernie Vaatstra
Keyword(s):  
New Zealand ◽  
North America ◽  
Pcr Primers ◽  
Ovis Aries ◽  
Bovine Papillomavirus ◽  
Detection Rates ◽  
Mesenchymal Neoplasms ◽  
Common Causes ◽  
Time Periods

Equine sarcoids are common mesenchymal neoplasms of horses that are caused by cross-species infection by deltapapillomaviruses. While bovine papillomavirus (BPV) 1 and 2 are the most common causes, there are differences between countries regarding which of these BPV types cause the majority of sarcoids. Additionally, no causative PV can be detected in a subset of sarcoids, suggesting that other PV types could be rarer causes of these neoplasms. In the present study, consensus PCR primers and PCR primers specific for the five deltapapillomavirus types currently known to cause mesenchymal neoplasia (BPV1, BPV2, BPV13, BPV14, and Ovis aries PV2 DNA) were used to investigate the presence of PV DNA in 104 sarcoids from three defined regions in New Zealand and from two distinct time periods separated by 15 years. PV DNA was detected in 94 (90.4%) sarcoids. Of the sarcoids containing PV DNA, 83 (88.3%) contained only BPV2 DNA, 9 (9.6%) BPV1 and BPV2 DNA, and 2 (2.1%) only BPV1 DNA. No other PV types were detected. The predominance of BPV2 is consistent with studies of sarcoids from North America but dissimilar to studies of sarcoids from Europe and Australia. Detection rates of BPV1 and BPV2 were similar in sarcoids from different regions of New Zealand and in sarcoids from different time periods. These results suggest that most equine sarcoids in New Zealand are caused by BPV2 and thus if vaccines are developed to prevent sarcoids, vaccines that provide good protection against BPV2 will be required in this country.

Bovine papillomavirus type-2 DNA and expression of E5 and E7 oncoproteins in vascular tumours of the urinary bladder in cattle

2007 ◽  
Vol 250 (1) ◽  
pp. 82-91 ◽  
Author(s):  
Giuseppe Borzacchiello ◽  
Valeria Russo ◽  
Claudia Spoleto ◽  
Sante Roperto ◽  
Liviu Balcos ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Bovine Papillomavirus

Association of bovine papillomavirus type-2 and urinary bladder tumours in cattle from Romania

2008 ◽  
Vol 85 (1) ◽  
pp. 145-148 ◽  
Author(s):  
L.G.F. Balcos ◽  
G. Borzacchiello ◽  
V. Russo ◽  
O. Popescu ◽  
S. Roperto ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Bovine Papillomavirus ◽  
Bladder Tumours

scholarly journals Detection of bovine papillomavirus type 2 in the peripheral blood of cattle with urinary bladder tumours: possible biological role

2008 ◽  
Vol 89 (12) ◽  
pp. 3027-3033 ◽  
Author(s):  
Sante Roperto ◽  
Roberto Brun ◽  
Francesca Paolini ◽  
Chiara Urraro ◽  
Valeria Russo ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Peripheral Blood ◽  
Bladder Tumour ◽  
Biological Role ◽  
Bovine Papillomavirus ◽  
Simultaneous Presence ◽  
Adult Cattle ◽  
Blood Samples ◽  
Bladder Tumours

Bovine papillomavirus type 2 (BPV-2) infection has been associated with urinary bladder tumours in adult cattle grazing on bracken fern-infested land. In this study, we investigated the simultaneous presence of BPV-2 in whole blood and urinary bladder tumours of adult cattle in an attempt to better understand the biological role of circulating BPV-2. Peripheral blood samples were collected from 78 cattle clinically suffering from a severe chronic enzootic haematuria. Circulating BPV-2 DNA was detected in 61 of them and in two blood samples from healthy cows. Fifty of the affected animals were slaughtered at public slaughterhouses and neoplastic proliferations in the urinary bladder were detected in all of them. BPV-2 DNA was amplified and sequenced in 78 % of urinary bladder tumour samples and in 38.9 % of normal samples as a control. Circulating episomal BPV-2 DNA was detected in 78.2 % of the blood samples. Simultaneous presence of BPV-2 DNA in neoplastic bladder and blood samples was detected in 37 animals. Specific viral E5 mRNA and E5 oncoprotein were also detected in blood by RT-PCR and Western blot/immunocytochemistry, respectively. It is likely that BPV-2 can persist and be maintained in an active status in the bloodstream, in particular in the lymphocytes, as a reservoir of viral infection that, in the presence of co-carcinogens, may cause the development of urinary bladder tumours.

Lymphoepithelioma-like Carcinoma of the Urinary Bladder in a Cow Associated with Bovine Papillomavirus Type-2

2008 ◽  
Vol 139 (2-3) ◽  
pp. 121-125 ◽  
Author(s):  
R. Brun ◽  
C. Urraro ◽  
C. Medaglia ◽  
V. Russo ◽  
G. Borzacchiello ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Bovine Papillomavirus
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