Bovine papillomavirus type-2 DNA and expression of E5 and E7 oncoproteins in vascular tumours of the urinary bladder in cattle

2007 ◽  
Vol 250 (1) ◽  
pp. 82-91 ◽  
Author(s):  
Giuseppe Borzacchiello ◽  
Valeria Russo ◽  
Claudia Spoleto ◽  
Sante Roperto ◽  
Liviu Balcos ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Bovine Papillomavirus

Bovine Papillomavirus Type-2 (BPV-2) Infection and Expression of Uroplakin IIIb, a Novel Urothelial Cell Marker, in Urinary Bladder Tumors of Cows

2005 ◽  
Vol 42 (6) ◽  
pp. 812-818 ◽  
Author(s):  
S. Roperto ◽  
V. Ambrosio ◽  
G. Borzacchiello ◽  
P. Galati ◽  
O. Paciello ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Single Cells ◽  
Urothelial Cell ◽  
Biological Behavior ◽  
Bovine Papillomavirus ◽  
Immunohistochemical Markers ◽  
Cell Layers ◽  
Urothelial Tumors ◽  
Differentiation Pattern

Several immunohistochemical markers have been used to define the differentiation pattern of urothelial cell tumors of the urinary bladder. We investigated the expression of the recently characterized uroplakin (UP) IIIb, an urothelium-specific and differentiation-dependent protein, in 39 urothelial tumors of the urinary bladder in cows that had suffered from chronic enzootic hematuria for several years. Bovine papillomavirus type 2 DNA was amplified and UP IIIb protein was detected in all these tumors. In papillomas and papillary carcinomas, UP IIIb expression was mostly seen as superficial staining; luminal and peripheral patterns were also observed. In nonpapillary carcinomas, UP IIIb appeared to define clearly the cell membrane lining intercellular and intracellular lumina as well as the cell borders in deeper cell layers. In benign and malignant lesions, an intracytoplasmic immunoreactivity was also detected. Coarse intracytoplasmic UP IIIb-positive material close to the nucleus occurred in some malignant cells. Focally strong membraneous immunostaining that marked single cells with complete ringlike peripheral pattern was seen. Although UP IIIb expression does not seem to correlate with the biological behavior of urothelial tumors, it appears to be a highly sensitive marker for bovine urothelial tumors.

scholarly journals Bovine papillomavirus type 2 infects the urinary bladder of water buffalo (Bubalus bubalis) and plays a crucial role in bubaline urothelial carcinogenesis

2013 ◽  
Vol 94 (2) ◽  
pp. 403-408 ◽  
Author(s):  
Sante Roperto ◽  
Valeria Russo ◽  
Ayhan Ozkul ◽  
Aylin Sepici-Dincel ◽  
Paola Maiolino ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Cell Transformation ◽  
Bubalus Bubalis ◽  
Immunofluorescence Assay ◽  
Receptor Interaction ◽  
Bovine Papillomavirus ◽  
Clinically Normal ◽  
Bladder Carcinogenesis ◽  
Β Receptor

Bovine papillomavirus type 2 (BPV-2) has been shown to infect and play a role in urinary bladder carcinogenesis of buffaloes grazed on pastures with ferns from the Marmara and Black Sea Regions of Turkey. BPV-2 DNA has been found in both neoplastic and non-neoplastic lesions of the urinary bladder. Furthermore, this virus may be a normal inhabitant of the urinary bladder since BPV-2 DNA has also been detected in clinically normal buffaloes. The viral activation by fern immunosuppressant or carcinogen may trigger the urothelial cell transformation. The E5 oncoprotein was solely detected in urothelial tumours and appeared to be co-localized with the overexpressed and phosphorylated platelet derived growth factor (PDGF) β receptor in a double-colour immunofluorescence assay. Our results indicate that the E5–PDGF β receptor interaction also occurs in spontaneous tumours of the bubaline urinary bladder, revealing an additional role of BPV-2 in bladder carcinogenesis of buffaloes.

scholarly journals Productive Infection of Bovine Papillomavirus Type 2 in the Urothelial Cells of Naturally Occurring Urinary Bladder Tumors in Cattle and Water Buffaloes

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62227 ◽  
Author(s):  
Sante Roperto ◽  
Valeria Russo ◽  
Ayhan Ozkul ◽  
Annunziata Corteggio ◽  
Aylin Sepici-Dincel ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Productive Infection ◽  
Bovine Papillomavirus ◽  
Urothelial Cells ◽  
Bladder Tumors ◽  
Water Buffaloes ◽  
Naturally Occurring

Association of bovine papillomavirus type-2 and urinary bladder tumours in cattle from Romania

2008 ◽  
Vol 85 (1) ◽  
pp. 145-148 ◽  
Author(s):  
L.G.F. Balcos ◽  
G. Borzacchiello ◽  
V. Russo ◽  
O. Popescu ◽  
S. Roperto ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Bovine Papillomavirus ◽  
Bladder Tumours

scholarly journals Detection of bovine papillomavirus type 2 in the peripheral blood of cattle with urinary bladder tumours: possible biological role

2008 ◽  
Vol 89 (12) ◽  
pp. 3027-3033 ◽  
Author(s):  
Sante Roperto ◽  
Roberto Brun ◽  
Francesca Paolini ◽  
Chiara Urraro ◽  
Valeria Russo ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Peripheral Blood ◽  
Bladder Tumour ◽  
Biological Role ◽  
Bovine Papillomavirus ◽  
Simultaneous Presence ◽  
Adult Cattle ◽  
Blood Samples ◽  
Bladder Tumours

Bovine papillomavirus type 2 (BPV-2) infection has been associated with urinary bladder tumours in adult cattle grazing on bracken fern-infested land. In this study, we investigated the simultaneous presence of BPV-2 in whole blood and urinary bladder tumours of adult cattle in an attempt to better understand the biological role of circulating BPV-2. Peripheral blood samples were collected from 78 cattle clinically suffering from a severe chronic enzootic haematuria. Circulating BPV-2 DNA was detected in 61 of them and in two blood samples from healthy cows. Fifty of the affected animals were slaughtered at public slaughterhouses and neoplastic proliferations in the urinary bladder were detected in all of them. BPV-2 DNA was amplified and sequenced in 78 % of urinary bladder tumour samples and in 38.9 % of normal samples as a control. Circulating episomal BPV-2 DNA was detected in 78.2 % of the blood samples. Simultaneous presence of BPV-2 DNA in neoplastic bladder and blood samples was detected in 37 animals. Specific viral E5 mRNA and E5 oncoprotein were also detected in blood by RT-PCR and Western blot/immunocytochemistry, respectively. It is likely that BPV-2 can persist and be maintained in an active status in the bloodstream, in particular in the lymphocytes, as a reservoir of viral infection that, in the presence of co-carcinogens, may cause the development of urinary bladder tumours.

Lymphoepithelioma-like Carcinoma of the Urinary Bladder in a Cow Associated with Bovine Papillomavirus Type-2

2008 ◽  
Vol 139 (2-3) ◽  
pp. 121-125 ◽  
Author(s):  
R. Brun ◽  
C. Urraro ◽  
C. Medaglia ◽  
V. Russo ◽  
G. Borzacchiello ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Bovine Papillomavirus

scholarly journals Bovine Papillomavirus Type 2 Infection and a Series of Mesenchymal Tumors of the Urinary Bladder in Cattle

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Manuela Martano ◽  
Franco Roperto ◽  
Rita de Cassia Stocco ◽  
Valeria Russo ◽  
Giuseppe Borzacchiello ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Vascular Tumors ◽  
Bovine Papillomavirus ◽  
Mesenchymal Tumors ◽  
Multinucleated Cells ◽  
E5 Oncoprotein ◽  
Papillary Endothelial Hyperplasia ◽  
Tumor Like Lesions ◽  
First Time

This report describes the histopathology of two hundred and fifty-three mesenchymal tumors of the urinary bladder in cattle grazing on lands rich in bracken fern. Approximately 80% were hemangiomas and angiosarcomas. Hemangioma (capillary, cavernous, and large vessels) was the most frequent mesenchymal tumor and was more common than angiosarcoma. Although the appearance of endothelial cells can vary remarkably, epithelioid angiosarcomas, often containing multinucleated cells, were the most frequent malignant vascular tumors. Hemangiopericytoma and tumors of muscle and soft connective tissue origin, alone and/or in association with tumor-like lesions, were less frequently seen. Furthermore, forty-five cases of intravascular papillary endothelial hyperplasia (IPEH), a lesion not previously reported in the urinary bladder of cattle, were also described. Bovine papillomavirus type-2 DNA was amplified in tumor samples. Forty vascular tumors were investigated by dual-labeling immunofluorescence, and, for the first time, a coexpression of E5 and platelet-derived growth factorβreceptor (PDGFβR) was shown to occur. The results show that the BPV-2 E5 oncoprotein binds to the activated form of the PDGFβreceptor thus playing an important role in mesenchymal as well as epithelial carcinogenesis of the urinary bladder. Furthermore, these findings demonstrate that BPV-2 infects both epithelial and mesenchymal cells.

Semi-nested PCR for detection and typing of bovine Papillomavirus type 2 in urinary bladder and whole blood from cattle with enzootic haematuria

2005 ◽  
Vol 126 (1-2) ◽  
pp. 215-219 ◽  
Author(s):  
Sheila Rezler Wosiacki ◽  
Marco Antônio Bacellar Barreiro ◽  
Alice Fernandes Alfieri ◽  
Amauri Alcindo Alfieri
Keyword(s):  
Urinary Bladder ◽  
Whole Blood ◽  
Nested Pcr ◽  
Bovine Papillomavirus

scholarly journals Association of Bovine Papillomavirus Type 2 (BPV-2) and Urinary Bladder Tumours in Cattle from the Azores Archipelago

2009 ◽  
Vol 141 (4) ◽  
pp. 305
Author(s):  
A.R. Resendes ◽  
S. Roperto ◽  
U. Chiara ◽  
F. Trapani ◽  
F. Roperto ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Bovine Papillomavirus ◽  
Azores Archipelago ◽  
Bladder Tumours

Evidence of Bovine Papillomavirus Type 2 in the Placenta of Cows with Urinary Bladder Tumours

2012 ◽  
Vol 146 (1) ◽  
pp. 58
Author(s):  
S. Roperto ◽  
R. Lucà ◽  
C. Urraro ◽  
I. Esposito ◽  
M. Riccardi ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Bovine Papillomavirus ◽  
Bladder Tumours
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