scholarly journals A simple genotyping method for CD247 3’‐untranslated region polymorphism rs1052231 and characterization of a reference cell panel

HLA ◽  
2021 ◽  
Author(s):  
Karima Al‐Akioui‐Sanz ◽  
Manuela Moraru ◽  
Carlos Vilches
Keyword(s):  
Untranslated Region ◽  
Reference Cell ◽  
Genotyping Method

scholarly journals Mutator-Suppressible Alleles of rough sheath1 and liguleless3 in Maize Reveal Multiple Mechanisms for Suppression

Genetics ◽  
2000 ◽  
Vol 154 (1) ◽  
pp. 437-446 ◽  
Author(s):  
Lisa Girard ◽  
Michael Freeling
Keyword(s):  
Untranslated Region ◽  
Mutant Allele ◽  
Negative Regulation ◽  
Wild Type ◽  
Knox Gene ◽  
Transcript Accumulation ◽  
Mu Suppression ◽  
Different Types ◽  
Rna Blot Analysis

Abstract Insertions of Mutator transposons into maize genes can generate suppressible alleles. Mu suppression is when, in the absence of Mu activity, the phenotype of a mutant allele reverts to that of its progenitor. Here we present the characterization of five dominant Mu-suppressible alleles of the knox (knotted1-like homeobox) genes liguleless3 and rough sheath1, which exhibit neomorphic phenotypes in the leaves. RNA blot analysis suggests that Mu suppression affects only the neomorphic aspect of the allele, not the wild-type aspect. Additionally, Mu suppression appears to be exerting its effects at the level of transcription or transcript accumulation. We show that truncated transcripts are produced by three alleles, implying a mechanism for Mu suppression of 5′ untranslated region insertion alleles distinct from that which has been described previously. Additionally, it is found that Mu suppression can be caused by at least three different types of Mutator elements. Evidence presented here suggests that whether an allele is suppressible or not may depend upon the site of insertion. We cite previous work on the knox gene kn1, and discuss our results in the context of interactions between Mu-encoded products and the inherently negative regulation of neomorphic liguleless3 and rough sheath1 transcription.

scholarly journals Molecular characterization of laccase genes from the basidiomycete Trametes hirsuta Bm-2 and analysis of the 5′ untranslated region (5′UTR)

3 Biotech ◽  
2019 ◽  
Vol 9 (4) ◽  
Author(s):  
Alejandrina Pereira-Patrón ◽  
Sara Solis-Pereira ◽  
Gabriel Lizama-Uc ◽  
Jorge H. Ramírez-Prado ◽  
Daisy Pérez-Brito ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Untranslated Region ◽  
Trametes Hirsuta ◽  
Laccase Genes

Characterization of the 3′ untranslated region of the human mu-opioid receptor (MOR-1) mRNA

Gene ◽  
2005 ◽  
Vol 364 ◽  
pp. 139-145 ◽  
Author(s):  
Soichiro Ide ◽  
Wenhua Han ◽  
Shinya Kasai ◽  
Harumi Hata ◽  
Ichiro Sora ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Untranslated Region ◽  
Mu Opioid Receptor ◽  
Mu Opioid

Differential expression of cold- and diet-specific genes encoding two carp liver Δ9-acyl-CoA desaturase isoforms

2003 ◽  
Vol 284 (1) ◽  
pp. R41-R50 ◽  
Author(s):  
S. D. Polley ◽  
P. E. Tiku ◽  
R. T. Trueman ◽  
M. X. Caddick ◽  
I. Y. Morozov ◽  
...  
Keyword(s):  
Untranslated Region ◽  
Dietary Treatment ◽  
Duplication Event ◽  
Transcript Levels ◽  
Induced Membrane ◽  
Small Segment ◽  
Fourfold Increase ◽  
Genes Encoding ◽  
Recent Duplication

Carp respond to cold by the upregulated expression of Δ9-acyl-CoA desaturase. Here we report the cloning and characterization of Cds2, a second Δ9-acyl CoA-desaturase expressed in carp liver. Both Cds1and Cds2 complemented the ole1 mutation in Saccharomyces cerevisiae, permitting the synthesis of Δ9-monounsaturates, confirming their identity as Δ9-desaturases. We demonstrate that under a standard feeding regime it is the Cds2, and not Cds1, transcript that is transiently upregulated during the first few days of cooling from 30°C to 10°C, the period when cold-induced membrane restructuring occurs. Cds2 exists as two differentially spliced transcripts, differing by a small segment from the 3′-untranslated region, the ratio of which varies with temperature. Feeding a diet enriched in saturated fats produced a fourfold increase in Cds1 transcript levels, which was blocked by cooling to 15°C. Cds2 transcript levels, however, showed no substantial response to the saturated diet. Thus carp liver uniquely expresses two isoforms of Δ9-acyl CoA desaturase, possibly formed by a recent duplication event, that are differentially regulated by cooling and dietary treatment.

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