Evaluation of nail surface topography using a three‐dimensional in vivo optical skin imaging system

2021 ◽  
Author(s):  
Hye Sung Han ◽  
Jong Hwan Kim ◽  
Tae‐Rin Kwon ◽  
Sung Eun Lee ◽  
Kwang Ho Yoo ◽  
...  
Keyword(s):  
Surface Topography ◽  
Imaging System ◽  
Three Dimensional ◽  
Skin Imaging

scholarly journals Fast in vivo bioluminescence tomography using a novel pure optical imaging technique

2017 ◽  
Vol 10 (03) ◽  
pp. 1750003 ◽  
Author(s):  
Shuang Zhang ◽  
Chengcai Leng ◽  
Hongbo Liu ◽  
Kun Wang ◽  
Jie Tian
Keyword(s):  
Optical Imaging ◽  
Animal Studies ◽  
Imaging System ◽  
Structural Information ◽  
Imaging Technique ◽  
Three Dimensional ◽  
Bioluminescence Tomography ◽  
3D Surface ◽  
Tumor Lesion

Bioluminescence tomography (BLT) is a novel optical molecular imaging technique that advanced the conventional planar bioluminescence imaging (BLI) into a quantifiable three-dimensional (3D) approach in preclinical living animal studies in oncology. In order to solve the inverse problem and reconstruct tumor lesions inside animal body accurately, the prior structural information is commonly obtained from X-ray computed tomography (CT). This strategy requires a complicated hybrid imaging system, extensive post imaging analysis and involvement of ionizing radiation. Moreover, the overall robustness highly depends on the fusion accuracy between the optical and structural information. Here, we present a pure optical bioluminescence tomographic (POBT) system and a novel BLT workflow based on multi-view projection acquisition and 3D surface reconstruction. This method can reconstruct the 3D surface of an imaging subject based on a sparse set of planar white-light and bioluminescent images, so that the prior structural information can be offered for 3D tumor lesion reconstruction without the involvement of CT. The performance of this novel technique was evaluated through the comparison with a conventional dual-modality tomographic (DMT) system and a commercialized optical imaging system (IVIS Spectrum) using three breast cancer xenografts. The results revealed that the new technique offered comparable in vivo tomographic accuracy with the DMT system ([Formula: see text]) in much shorter data analysis time. It also offered significantly better accuracy comparing with the IVIS system ([Formula: see text]) without sacrificing too much time.

scholarly journals Ranges of Cervical Intervertebral Disc Deformation During an In Vivo Dynamic Flexion–Extension of the Neck

2017 ◽  
Vol 139 (6) ◽  
Author(s):  
Yan Yu ◽  
Haiqing Mao ◽  
Jing-Sheng Li ◽  
Tsung-Yuan Tsai ◽  
Liming Cheng ◽  
...  
Keyword(s):  
Cervical Spine ◽  
Imaging System ◽  
Human Subjects ◽  
Three Dimensional ◽  
Cervical Disc ◽  
Significant Difference ◽  
Flexion Extension ◽  
Fluoroscopic Imaging ◽  
Magnetic Resonance Imaging Mri

While abnormal loading is widely believed to cause cervical spine disc diseases, in vivo cervical disc deformation during dynamic neck motion has not been well delineated. This study investigated the range of cervical disc deformation during an in vivo functional flexion–extension of the neck. Ten asymptomatic human subjects were tested using a combined dual fluoroscopic imaging system (DFIS) and magnetic resonance imaging (MRI)-based three-dimensional (3D) modeling technique. Overall disc deformation was determined using the changes of the space geometry between upper and lower endplates of each intervertebral segment (C3/4, C4/5, C5/6, and C6/7). Five points (anterior, center, posterior, left, and right) of each disc were analyzed to examine the disc deformation distributions. The data indicated that between the functional maximum flexion and extension of the neck, the anterior points of the discs experienced large changes of distraction/compression deformation and shear deformation. The higher level discs experienced higher ranges of disc deformation. No significant difference was found in deformation ranges at posterior points of all the discs. The data indicated that the range of disc deformation is disc level dependent and the anterior region experienced larger changes of deformation than the center and posterior regions, except for the C6/7 disc. The data obtained from this study could serve as baseline knowledge for the understanding of the cervical spine disc biomechanics and for investigation of the biomechanical etiology of disc diseases. These data could also provide insights for development of motion preservation surgeries for cervical spine.

A New High Frequency Ultrasound Skin Imaging System: Imaging Properties and Clinical in Vivo Results

2007 ◽  
pp. 137-144 ◽  
Author(s):  
M. Vogt ◽  
R. Scharenberg ◽  
G. Moussa ◽  
M. Sand ◽  
K. Hoffmann ◽  
...  
Keyword(s):  
High Frequency ◽  
Imaging System ◽  
High Frequency Ultrasound ◽  
Skin Imaging ◽  
Imaging Properties ◽  
Frequency Ultrasound

scholarly journals Hydrojet-based delivery of footprint-free iPSC-derived cardiomyocytes into porcine myocardium

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Marbod Weber ◽  
Andreas Fech ◽  
Luise Jäger ◽  
Heidrun Steinle ◽  
Louisa Bühler ◽  
...  
Keyword(s):  
Imaging System ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Pressure Setting ◽  
Infarcted Myocardium ◽  
Efficient Delivery ◽  
Intramyocardial Delivery ◽  
Induced Pluripotent

Abstract The reprogramming of patient´s somatic cells into induced pluripotent stem cells (iPSCs) and the consecutive differentiation into cardiomyocytes enables new options for the treatment of infarcted myocardium. In this study, the applicability of a hydrojet-based method to deliver footprint-free iPSC-derived cardiomyocytes into the myocardium was analyzed. A new hydrojet system enabling a rapid and accurate change between high tissue penetration pressures and low cell injection pressures was developed. Iron oxide-coated microparticles were ex vivo injected into porcine hearts to establish the application parameters and the distribution was analyzed using magnetic resonance imaging. The influence of different hydrojet pressure settings on the viability of cardiomyocytes was analyzed. Subsequently, cardiomyocytes were delivered into the porcine myocardium and analyzed by an in vivo imaging system. The delivery of microparticles or cardiomyocytes into porcine myocardium resulted in a widespread three-dimensional distribution. In vitro, 7 days post-injection, only cardiomyocytes applied with a hydrojet pressure setting of E20 (79.57 ± 1.44%) showed a significantly reduced cell viability in comparison to the cells applied with 27G needle (98.35 ± 5.15%). Furthermore, significantly less undesired distribution of the cells via blood vessels was detected compared to 27G needle injection. This study demonstrated the applicability of the hydrojet-based method for the intramyocardial delivery of iPSC-derived cardiomyocytes. The efficient delivery of cardiomyocytes into infarcted myocardium could significantly improve the regeneration.

Imaging System for Three-Dimensional Mapping of Cerebrocortical Capillary Networks in Vivo

1993 ◽  
Vol 46 (3) ◽  
pp. 293-309 ◽  
Author(s):  
Antal G. Hudetz ◽  
Andrew S. Greene ◽  
Gabriella Fehér ◽  
Derek E. Knuese ◽  
Allen W. Cowley
Keyword(s):  
Imaging System ◽  
Three Dimensional ◽  
Capillary Networks ◽  
Three Dimensional Mapping ◽  
Dimensional Mapping

scholarly journals Investigation of Geometric Deformations of The Lumbar Disc During Axial Body Rotations

2021 ◽  
Author(s):  
Haoxiang Xu ◽  
Wangqiang Wen ◽  
Zepei Zhang ◽  
Jianqiang Bai ◽  
Bowen Kou ◽  
...  
Keyword(s):  
Lumbar Spine ◽  
Imaging System ◽  
Lumbar Disc ◽  
Three Dimensional ◽  
Lumbar Intervertebral Disc ◽  
Fluoroscopic Imaging ◽  
Extra Weight ◽  
The Right ◽  
Deformation Patterns

Abstract BackgroundQuantitative data on in vivo vertebral disc deformations are critical for enhancing our understanding of spinal pathology and improving the design of surgical materials. This study investigated in vivo lumbar intervertebral disc deformations during axial rotations under different load-bearing conditions.MethodsTwelve healthy subjects (7 males and 5 females) between the ages of 25 and 39 were recruited. Using a combination of a dual fluoroscopic imaging system (DFIS) and CT, the images of L3-5 segments scanned by CT were transformed into three-dimensional models, which matched the instantaneous images of the lumbar spine taken by a double fluorescent X-ray system during axial rotations to reproduce motions. Then, the kinematic data of the compression and shear deformations of the lumbar disc and the coupled bending of the vertebral body were obtained.ResultsRelative to the supine position, the average compression deformation caused by rotation is between +10% and -40%, and the shear deformation is between 17% and 50%. Under physiological weightbearing loads, different levels of lumbar discs exhibit similar deformation patterns, and the deformation patterns of left and right rotations are approximately symmetrical. The deformation patterns change significantly under a 10 kg load, with the exception of the L3-4 disc during the right rotation.ConclusionThe deformation of the lumbar disc was direction-specific and level-specific during axial rotations and was affected by extra weight. These data can provide new insights into the biomechanics of the lumbar spine and optimize the parameters of artificial lumbar spine devices.

The Effects of the Surface Topography of Micromachined Titanium Substrata on Cell Behavior in Vitro and in Vivo

1999 ◽  
Vol 121 (1) ◽  
pp. 49-57 ◽  
Author(s):  
D. M. Brunette ◽  
B. Chehroudi
Keyword(s):  
Surface Topography ◽  
Fibrous Tissue ◽  
Three Dimensional ◽  
Tissue Response ◽  
Cell Behavior ◽  
Tissue Organization ◽  
Dimensional Reconstruction ◽  
Microfabrication Techniques

Surface properties, including topography and chemistry, are of prime importance in establishing the response of tissues to biomaterials. Microfabrication techniques have enabled the production of precisely controlled surface topographies that have been used as substrata for cells in culture and on devices implanted in vivo. This article reviews aspects of cell behavior involved in tissue response to implants with an emphasis on the effects of topography. Microfabricated grooved surfaces produce orientation and directed locomotion of epithelial cells in vitro and can inhibit epithelial downgrowth on implants. The effects depend on the groove dimensions and they are modified by epithelial cell–cell interactions. Fibroblasts similarly exhibit contact guidance on grooved surfaces, but fibroblast shape in vitro differs markedly from that found in vivo. Surface topography is important in establishing tissue organization adjacent to implants, with smooth surfaces generally being associated with fibrous tissue encapsulation. Grooved topographies appear to have promise in reducing encapsulation in the short term, but additional studies employing three-dimensional reconstruction and diverse topographies are needed to understand better the process of connective-tissue organization adjacent to implants. Microfabricated surfaces can increase the frequency of mineralized bone-like tissue nodules adjacent to subcutaneously implanted surfaces in rats. Orientation of these nodules with grooves occurs both in culture and on implants. Detailed comparisons of cell behavior on micromachined substrata in vitro and in vivo are difficult because of the number and complexity of factors, such as population density and micromotion, that can differ between these conditions.

scholarly journals 3D cellular-resolution imaging in arteries using few-mode interferometry

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Biwei Yin ◽  
Zhonglie Piao ◽  
Kensuke Nishimiya ◽  
Chulho Hyun ◽  
Joseph A. Gardecki ◽  
...  
Keyword(s):  
Imaging System ◽  
Three Dimensional ◽  
Numerical Aperture ◽  
Inflammatory Cells ◽  
Intravascular Imaging ◽  
Dependent Manner ◽  
Depth Of Focus ◽  
Cross Sectional ◽  
Cellular Resolution

AbstractCross-sectional visualisation of the cellular and subcellular structures of human atherosclerosis in vivo is significant, as this disease is fundamentally caused by abnormal processes that occur at this scale in a depth-dependent manner. However, due to the inherent resolution-depth of focus tradeoff of conventional focusing optics, today’s highest-resolution intravascular imaging technique, namely, optical coherence tomography (OCT), is unable to provide cross-sectional images at this resolution through a coronary catheter. Here, we introduce an intravascular imaging system and catheter based on few-mode interferometry, which overcomes the depth of focus limitation of conventional high-numerical-aperture objectives and enables three-dimensional cellular-resolution intravascular imaging in vivo by a submillimetre diameter, flexible catheter. Images of diseased cadaver human coronary arteries and living rabbit arteries were acquired with this device, showing clearly resolved cellular and subcellular structures within the artery wall, such as individual crystals, smooth muscle cells, and inflammatory cells. The capability of this technology to enable cellular-resolution, cross-sectional intravascular imaging will make it possible to study and diagnose human coronary disease with much greater precision in the future.

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