scholarly journals Pattern of human monocyte subpopulations in health and disease

2020 ◽  
Vol 92 (1) ◽  
Author(s):  
Agnieszka Ożańska ◽  
Donata Szymczak ◽  
Justyna Rybka
Keyword(s):  
Human Monocyte ◽  
Health And Disease ◽  
Monocyte Subpopulations

scholarly journals Differential interleukin-1 elaboration by density-defined human monocyte subpopulations

Blood ◽  
1985 ◽  
Vol 66 (2) ◽  
pp. 298-301
Author(s):  
JA Elias ◽  
P Chien ◽  
KM Gustilo ◽  
AD Schreiber
Keyword(s):  
Human Blood ◽  
Interleukin 1 ◽  
Human Monocyte ◽  
Mononuclear Phagocytes ◽  
Blood Monocyte ◽  
Thymocyte Proliferation ◽  
Percoll Gradients ◽  
Human Blood Monocyte ◽  
Monocytes And Macrophages ◽  
Monocyte Subpopulations

Interleukin-1 (IL-1) is an important immunoregulatory peptide produced by monocytes and macrophages. Because mononuclear phagocytes are morphologically and functionally heterogeneous, we examined whether they differ in their ability to elaborate IL-1. We used discontinuous Percoll gradients to obtain five density-defined human blood monocyte subpopulations. Unfractionated monocytes and their subsets were compared for their ability to stimulate thymocyte proliferation. Supernatants obtained from the denser monocytes consistently contained more IL-1 activity than did supernatants from the less dense cells. This difference in IL-1 activity was the result of differences in IL-1 elaboration, not the selective production of an inhibitor of IL-1- induced thymocyte proliferation. These data demonstrate that density- defined human monocyte subpopulations differ in their capacity to elaborate IL-1.

The three human monocyte subsets: implications for health and disease

2012 ◽  
Vol 53 (1-3) ◽  
pp. 41-57 ◽  
Author(s):  
Kok Loon Wong ◽  
Wei Hseun Yeap ◽  
June Jing Yi Tai ◽  
Siew Min Ong ◽  
Truong Minh Dang ◽  
...  
Keyword(s):  
Human Monocyte ◽  
Monocyte Subsets ◽  
Health And Disease

scholarly journals Differential interleukin-1 elaboration by density-defined human monocyte subpopulations

Blood ◽  
1985 ◽  
Vol 66 (2) ◽  
pp. 298-301 ◽  
Author(s):  
JA Elias ◽  
P Chien ◽  
KM Gustilo ◽  
AD Schreiber
Keyword(s):  
Human Blood ◽  
Interleukin 1 ◽  
Human Monocyte ◽  
Mononuclear Phagocytes ◽  
Blood Monocyte ◽  
Thymocyte Proliferation ◽  
Percoll Gradients ◽  
Human Blood Monocyte ◽  
Monocytes And Macrophages ◽  
Monocyte Subpopulations

Abstract Interleukin-1 (IL-1) is an important immunoregulatory peptide produced by monocytes and macrophages. Because mononuclear phagocytes are morphologically and functionally heterogeneous, we examined whether they differ in their ability to elaborate IL-1. We used discontinuous Percoll gradients to obtain five density-defined human blood monocyte subpopulations. Unfractionated monocytes and their subsets were compared for their ability to stimulate thymocyte proliferation. Supernatants obtained from the denser monocytes consistently contained more IL-1 activity than did supernatants from the less dense cells. This difference in IL-1 activity was the result of differences in IL-1 elaboration, not the selective production of an inhibitor of IL-1- induced thymocyte proliferation. These data demonstrate that density- defined human monocyte subpopulations differ in their capacity to elaborate IL-1.

Differences in superoxide production by nonmigrating and migrating human monocyte subpopulations

1982 ◽  
Vol 108 (1) ◽  
pp. 392-398 ◽  
Author(s):  
Liana Harvath ◽  
Janis K. Lazdins ◽  
Enrica Alteri ◽  
Edward J. Leonard
Keyword(s):  
Human Monocyte ◽  
Superoxide Production ◽  
Monocyte Subpopulations

P5394Whole transcriptome sequencing to define monocyte subpopulation-specific lncRNA profiles in acute myocardial infarction patients and healthy controls

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
A Kratzer ◽  
H Giral Arnal ◽  
V Franke ◽  
M Moobed ◽  
A Akalin ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Cardiovascular Disease ◽  
Acute Myocardial Infarction ◽  
Next Generation Sequencing ◽  
Human Monocyte ◽  
Circular Rnas ◽  
Next Generation ◽  
Monocyte Subpopulations ◽  
Non Coding Rnas ◽  
Generation Sequencing

Abstract Background Monocytes are important immune cells in both onset and resolution of inflammation during pathologies such as acute myocardial infarction (AMI) and atherosclerosis. Long non-coding RNAs (lncRNAs) have emerged as novel regulatory and highly cell-specific molecules that can modulate cell physiology in numerous ways such as mRNA (de-)stabilization, micro RNA sponging or scaffolding of RNA binding proteins. Purpose Define a complete human monocyte subpopulation-specific transcriptome of long non-coding RNAs and characterize the difference in the profile of these RNA molecules in AMI patients. Methods and results Human monocyte subpopulations, defined as classical, intermediate and non-classical based on the expression of the surface markers CD14 and CD16, were collected on a FACS Aria II. Ribosomal-depleted cDNA libraries generated from total RNA were processed for Next Generation Sequencing on a HiSeq Illumina 2000. Computationally intensive bioinformatics revealedannotated lncRNAs, antisense, pseudogene and circular RNAs with significant difference in their expression profiles within subpopulations of healthy donors such as MEG3 or TERC, potential role players in cardiovascular disease. Our data also unraveled novel non-annotated ncRNAs not yet reported to reference databases, which are expected to be monocyte-specific. We applied certain criteria to identify potential candidate molecules such as annotation with existing Ensembl ID and a pre-determined expression level. Thereupon we selected differentially regulated long non-coding RNAs differentially expressed in cardiovascular disease and discovered 18 annotated potential lncRNAs dysregulated in classical monocytes of AMI patients such as HLX antisense, which might be involved in monocyte differentiation. Additional 5 targets appeared specific only for differences in intermediate and 3 with additional specific differences only in non-classical monocytes.Real-time PCR was applied for validation of long non-coding linear and circular RNAs differential expression and also to determine their nucleocytoplasmic distribution. We observed preferential nuclear expression for most lncRNAs in contrast to cytoplasmic circRNAs. In vitro assays for silencing and overexpressing certain target molecules as well astreatment withinflammatory stimuli and in silicoanalysis with different bioinformatics tools such as FANTOM and UCSC browser will help to unravel their functionality. Conclusions Next generation sequencing allowed us to define a human monocyte subpopulation-specific long non-coding transcriptome that presented significant differences in both lncRNA and circRNAs within monocyte subpopulations of healthy subjects and AMI patients. Studying functional mechanisms of identified lncRNAs and their interaction with the coding genome will help to unravel novel regulatory means of monocytes in acute myocardial infarction providing new opportunities for therapeutic approaches. Acknowledgement/Funding DZHK (German Centre for Cardiovascular Research), partner site Berlin, Germany;Berlin Institute of Health (BIH);Swiss National Science Foundation (SNF)

scholarly journals High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human Monocytes In Vitro

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Johanna L. Grün ◽  
Aaron N. Manjarrez-Reyna ◽  
Angélica Y. Gómez-Arauz ◽  
Sonia Leon-Cabrera ◽  
Felix Rückert ◽  
...  
Keyword(s):  
Risk Factors ◽  
Metabolic Syndrome ◽  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Human Monocyte ◽  
High Density ◽  
Human Monocytes ◽  
Low Hdl ◽  
Monocyte Subpopulations

The effect of metabolic syndrome on human monocyte subpopulations has not yet been studied. Our main goal was to examine monocyte subpopulations in metabolic syndrome patients, while also identifying the risk factors that could directly influence these cells. Eighty-six subjects were divided into metabolic syndrome patients and controls. Monocyte subpopulations were quantified by flow cytometry, and interleukin- (IL-) 1β secretion levels were measured by ELISA. Primary human monocytes were cultured in low or elevated concentrations of high-density lipoprotein (HDL) and stimulated with lipopolysaccharide (LPS). The nonclassical monocyte (NCM) percentage was significantly increased in metabolic syndrome patients as compared to controls, whereas classical monocytes (CM) were reduced. Among all metabolic syndrome risk factors, HDL reduction exhibited the most important correlation with monocyte subpopulations and then was studied in vitro. Low HDL concentration reduced the CM percentage, whereas it increased the NCM percentage and IL-1β secretion in LPS-treated monocytes. The LPS effect was abolished when monocytes were cultured in elevated HDL concentrations. Concurring with in vitro results, IL-1β serum values significantly increased in metabolic syndrome patients with low HDL levels as compared to metabolic syndrome patients without HDL reduction. Our data demonstrate that HDL directly modulates monocyte subpopulations in metabolic syndrome.

scholarly journals Differential chemokine receptor expression and function in human monocyte subpopulations

2000 ◽  
Vol 67 (5) ◽  
pp. 699-704 ◽  
Author(s):  
Christian Weber ◽  
Kai-Uwe Belge ◽  
Philipp von Hundelshausen ◽  
Georg Draude ◽  
Birgit Steppich ◽  
...  
Keyword(s):  
Chemokine Receptor ◽  
Human Monocyte ◽  
Receptor Expression ◽  
Chemokine Receptor Expression ◽  
Monocyte Subpopulations ◽  
And Function

scholarly journals Functional characterization of the biogenic amine transporter system on human macrophages

2021 ◽  
Author(s):  
Phillip M Mackie ◽  
Adithya Gopinath ◽  
Dominic M Montas ◽  
Alyssa Nielsen ◽  
Rachel Nolan ◽  
...  
Keyword(s):  
Biogenic Amine ◽  
Functional Characterization ◽  
Human Monocyte ◽  
Paracrine Signaling ◽  
Macrophage Response ◽  
Reverse Transport ◽  
The Central Nervous System ◽  
Health And Disease

AbstractMonocyte-derived macrophages are key players in tissue homeostasis and disease regulated by a variety of signaling molecules. Recent literature has highlighted the ability for biogenic amines to regulate macrophage functions, but the mechanisms governing biogenic amine signaling on and around immune cells remains nebulous. In the central nervous system, biogenic amine transporters are regarded as the master regulators of neurotransmitter signaling. While we and others have shown macrophages express these transporters, relatively little is known of their function on these cells. To address these knowledge gaps, we interrogated the function of norepinephrine (NET) and dopamine (DAT) transporters on human monocyte-derived macrophages. We found that both NET and DAT are present and can uptake substrate from the extracellular space at baseline. Not only was DAT expressed in cultured macrophages, but it was also detected in a subset of intestinal macrophages in situ. Surprisingly, we discovered a NET-independent, DAT-mediated immuno-modulatory mechanism in response to lipopolysaccharide (LPS). LPS induced reverse transport of dopamine through DAT, engaging autocrine/paracrine signaling loop that regulated the macrophage response. Removing this signaling loop enhanced the pro-inflammatory response to LPS. Finally, we found that this DAT-immune axis was disrupted in disease. Collectively, our data introduce a novel role for DAT in the regulation of innate immunity during health and disease.

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