scholarly journals Mannose‐capped lipoarabinomannan‐induced B10 cells decrease severity of dextran sodium sulphate‐induced inflammatory bowel disease in mice

2019 ◽  
Vol 91 (2) ◽  
Author(s):  
Chun-Hui Yuan ◽  
Xin Li ◽  
Liang Luo ◽  
Ya-Ping Wang ◽  
Dong-Li Zhang ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Sodium Sulphate ◽  
Dextran Sodium Sulphate ◽  
Inflammatory Bowel ◽  
B10 Cells

scholarly journals The Effect of Lactobacillus casei on Experimental Porcine Inflammatory Bowel Disease Induced by Dextran Sodium Sulphate

2021 ◽  
Vol 64 (2) ◽  
pp. 85-90
Author(s):  
Jan Bureš ◽  
Darina Kohoutová ◽  
Jaroslav Květina ◽  
Věra Radochová ◽  
Michal Pavlík ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Transverse Colon ◽  
Lactobacillus Casei ◽  
Statistical Significance ◽  
Probiotic Strain ◽  
Sodium Sulphate ◽  
Dextran Sodium Sulphate ◽  
Inflammatory Bowel ◽  
Histopathological Score

Background: Gastrointestinal injury caused by dextran sodium sulphate (DSS) is a reliable porcine experimental model of inflammatory bowel disease (IBD). The purpose of this study was to evaluate the effect of probiotic Lactobacillus casei DN 114001 (LC) on DSS-induced experimental IBD.Results: Eighteen female pigs (Sus scrofa f. domestica, weight 33–36 kg, age 4–5 months) were divided into 3 groups (6 animals per group): controls with no treatment, DSS, and DSS + LC. LC was administered to overnight fasting animals in a dietary bolus in the morning on days 1–7 (4.5 × 1010 live bacteria/day). DSS was applied simultaneously on days 3–7 (0.25 g/kg/day). On day 8, the pigs were sacrificed. Histopathological score and length of crypts/glands (stomach, jejunum, ileum, transverse colon), length and width of villi (jejunum, ileum), and mitotic and apoptotic indices (jejunum, ileum, transverse colon) were assessed. DSS increased the length of glands in the stomach, length of crypts and villi in the jejunum and ileum, and the histopathological score of gastrointestinal damage, length of crypts and mitotic activity in the transverse colon. Other changes did not achieve any statistical significance. Administration of LC reduced the length of villi in the jejunum and ileum to control levels and decreased the length of crypts in the jejunum. Conclusions: Treatment with a probiotic strain of LC significantly accelerated regeneration of the small intestine in a DSS-induced experimental porcine model of IBD.

scholarly journals Dextran Sodium Sulphate Colitis Mouse Model: Traps and Tricks

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Martina Perše ◽  
Anton Cerar
Keyword(s):  
Inflammatory Bowel Disease ◽  
Animal Models ◽  
Bowel Disease ◽  
Sodium Sulphate ◽  
Unknown Etiology ◽  
Dextran Sulphate ◽  
Dextran Sodium Sulphate ◽  
Wide Range ◽  
Inflammatory Bowel ◽  
Colitis Model

Inflammatory bowel disease (IBD) is a complex multifactorial disease of unknown etiology. Thus, dozens of different animal models of IBD have been developed in past decades. Animal models of IBD are valuable and indispensable tools that provide a wide range of options for investigating involvement of various factors into the pathogenesis of IBD and to evaluate different therapeutic options. However, the dextran sulphate sodium (DSS-) induced colitis model has some advantages when compared to other animal models of colitis. It is well appreciated and widely used model of inflammatory bowel disease because of its simplicity. It has many similarities to human IBD, which are mentioned in the paper. In spite of its simplicity and wide applicability, there are also traps that need to be taken into account when using DSS model. As demonstrated in the present paper, various factors may affect susceptibility to DSS-induced lesions and modify results.

scholarly journals Effects of arachidonic acid intake on inflammatory reactions in dextran sodium sulphate-induced colitis in rats

2015 ◽  
Vol 114 (5) ◽  
pp. 734-745 ◽  
Author(s):  
Yukiko Naito ◽  
Xu Ji ◽  
Shigehiro Tachibana ◽  
Satoko Aoki ◽  
Mami Furuya ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Arachidonic Acid ◽  
Bowel Disease ◽  
Sodium Sulphate ◽  
Myeloperoxidase Activity ◽  
Contrast Administration ◽  
Inflammatory Reactions ◽  
Male Wistar Rats ◽  
Mucosal Oedema ◽  
Inflammatory Bowel

AbstractThe aim of this study was to investigate the effects of the administration of oral arachidonic acid (AA) in rats with or without dextran sulphate sodium (DSS)-induced inflammatory bowel disease. Male Wistar rats were administered AA at 0, 5, 35 or 240 mg/kg daily by gavage for 8 weeks. Inflammatory bowel disease was induced by replacing drinking water with 3 % DSS solution during the last 7 d of the AA dosing period. These animals passed loose stools, diarrhoea and red-stained faeces. Cyclo-oxygenase-2 concentration and myeloperoxidase activity in the colonic tissue were significantly increased in the animals given AA at 240 mg/kg compared with the animals given AA at 0 mg/kg. Thromboxane B2 concentration in the medium of cultured colonic mucosae isolated from these groups was found to be dose-dependently increased by AA, and the increase was significant at 35 and 240 mg/kg. Leukotriene B4 concentration was also significantly increased and saturated at 5 mg/kg. In addition, AA at 240 mg/kg promoted DSS-induced colonic mucosal oedema with macrophage infiltration. In contrast, administration of AA for 8 weeks, even at 240 mg/kg, showed no effects on the normal rats. These results suggest that in rats with bowel disease AA metabolism is affected by oral AA, even at 5 mg/kg per d, and that excessive AA may aggravate inflammation, whereas AA shows no effects in rats without inflammatory bowel disease.

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