scholarly journals A hypothesis for the existence of two types of tuberculosis, reflecting two distinct types of immune failure to control the pathogen, based upon prevalence of mycobacterium-specific IgG subclasses

2018 ◽  
Vol 87 (6) ◽  
pp. e12665 ◽  
Author(s):  
J. Menon ◽  
V. H. Hoeppner ◽  
A. Judd ◽  
C. A. Power ◽  
P. A. Bretscher
Keyword(s):  
Igg Subclasses ◽  
Specific Igg ◽  
Immune Failure

scholarly journals The Characterization of Disease Severity Associated IgG Subclasses Response in COVID-19 Patients

2021 ◽  
Vol 12 ◽  
Author(s):  
Huanle Luo ◽  
Tingting Jia ◽  
Jiamin Chen ◽  
Shike Zeng ◽  
Zengzhao Qiu ◽  
...  
Keyword(s):  
Viral Load ◽  
Disease Severity ◽  
Young Patients ◽  
Neutralizing Antibody ◽  
Igg Subclasses ◽  
Nasopharyngeal Swab ◽  
Obvious Effect ◽  
Old Patients ◽  
Biological Sex ◽  
Specific Igg

Increasing evidence suggests that dysregulated immune responses are associated with the clinical outcome of coronavirus disease 2019 (COVID-19). Nucleocapsid protein (NP)-, spike (S)-, receptor binding domain (RBD)- specific immunoglobulin (Ig) isotypes, IgG subclasses and neutralizing antibody (NAb) were analyzed in 123 serum from 63 hospitalized patients with severe, moderate, mild or asymptomatic COVID-19. Mild to modest correlations were found between disease severity and antigen specific IgG subclasses in serum, of which IgG1 and IgG3 were negatively associated with viral load in nasopharyngeal swab. Multiple cytokines were significantly related with antigen-specific Ig isotypes and IgG subclasses, and IL-1β was positively correlated with most antibodies. Furthermore, the old patients (≥ 60 years old) had higher levels of chemokines, increased NAb activities and SARS-CoV-2 specific IgG1, and IgG3 responses and compromised T cell responses compared to the young patients (≤ 18 years old), which are related with more severe cases. Higher IgG1 and IgG3 were found in COVID-19 patients with comorbidities while biological sex had no effect on IgG subclasses. Overall, we have identified diseases severity was related to higher antibodies, of which IgG subclasses had weakly negative correlation with viral load, and cytokines were significantly associated with antibody response. Further, advancing age and comorbidities had obvious effect on IgG1 and IgG3.

P4-105: HSV-1 Specific IGG Subclasses in Individuals with Alzheimer’s Disease

2016 ◽  
Vol 12 ◽  
pp. P1052-P1053 ◽  
Author(s):  
Roberta Mancuso ◽  
Simone Agostini ◽  
Elena Calabrese ◽  
Ambra Hernis ◽  
Raffaello Nemni ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Igg Subclasses ◽  
Specific Igg ◽  
Hsv 1

77 Elevated shrimp-specific IGG subclasses in shrimp sensitive individuals

1988 ◽  
Vol 81 (1) ◽  
pp. 188
Author(s):  
JE Morgan ◽  
CB Daul ◽  
S Willard ◽  
SB Lehrer
Keyword(s):  
Igg Subclasses ◽  
Specific Igg

Selection and characterisation of FVIII-specific single chain variable fragments

2013 ◽  
Vol 33 (S 01) ◽  
pp. S39-S45 ◽  
Author(s):  
A. Naumann ◽  
A. K. Scherger ◽  
J. Neuwirth ◽  
A. Orlowski ◽  
J. Kahle ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Tolerance Induction ◽  
Igg Subclasses ◽  
Igg Subclass ◽  
Single Chain ◽  
Relative Contribution ◽  
Phage Display Libraries ◽  
Specific Igg ◽  
Single Chain Variable Fragments ◽  
A2 Domain

SummaryThe development of inhibitory anti-FVIII antibodies is currently the most severe complication in the treatment of haemophilia A patients. Inhibitor eradication can be achieved by immune tolerance induction (ITI). Recent findings suggest a correlation between the FVIII-specific IgG subclass distribution and the duration or outcome of ITI. To quantify FVIII-specific IgG subclasses in patients’ plasma FVIII-specific IgG standards are required. Here, the isolation of FVIII-specific single chain variable fragments (scFvs) from synthetic phage display libraries and the characterisation of their FVIII domain specificity are described. The isolated scFv 1G10, which binds to the FVIII A2 domain, was cloned into the context of the four human IgG (hIgG) subclasses and expressed in mammalian cells. Purified 1G10-hIgG1, -hIgG2, -hIgG3 and -hIgG4 are used as standards to determine the absolute amounts and relative contribution of the different FVIII-specific IgG subclasses in future studies. The results from these studies will eventually add to understanding the role of the FVIII-specific IgG subclass distribution as prognostic factor for the outcome of ITI.

scholarly journals Anti-HLA Donor-Specific IgG Subclasses and C1q-binding Evolution in Posttransplant Monitoring

2018 ◽  
Vol 4 (9) ◽  
pp. e385 ◽  
Author(s):  
Renata von Glehn Ponsirenas ◽  
Helena B. Cazarote ◽  
Stanley de Almeida Araújo ◽  
David Campos Wanderley ◽  
Silvia Shimakura ◽  
...  
Keyword(s):  
Igg Subclasses ◽  
Specific Igg

Immunoglobulin G (IgG) and IgG subclass reference intervals in children, using Optilite® reagents

2018 ◽  
Vol 56 (8) ◽  
pp. 1319-1327 ◽  
Author(s):  
Olivier Grunewald ◽  
Benjamin Lopez ◽  
Séverine Brabant ◽  
Stéphanie Rogeau ◽  
Antoine Deschildre ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Age Groups ◽  
Reference Intervals ◽  
Igg Subclasses ◽  
Specific Reference ◽  
Igg Subclass ◽  
Serum Samples ◽  
Gender Specific Differences ◽  
Specific Igg ◽  
Changes Over Time

Abstract Background: Immunoglobulin G (IgG) and IgG subclass assays are indicated in patients with suspected primary immunodeficiency (PID). Commercially available assays for IgG subclass determination are calibrated against various preparations, and so specific reference values are required for each of them. Using Optilite® reagents from The Binding Site Group Ltd., we sought to determine the pediatric IgG and IgG subclass reference intervals with respect to the ERM-DA470k certified reference material. Methods: Levels of IgG and IgG subclasses were analyzed in serum samples collected from a large cohort of PID-free children and adolescents. Reference intervals were calculated for previously published age groups (6–12 months, 12–18 months, 18 months–2 years, 2–3 years, 3–4 years, 4–6 years, 6–9 years, 9–12 years and 12–18 years), according to the Clinical and Laboratory Standards Institute’s C28-A3c protocol. Results: A total of 456 serum samples were analyzed. The correlation between the total IgG and the sum of the IgG subclasses was good (r2=0.96). No statistically significant gender-specific differences were observed. Our results for the changes over time in IgG and IgG subclass levels are consistent with previous reports. The differences between our lower/upper reference limits and those in the literature are probably due to variations in calibration. Conclusions: Our present results provide a reliable basis for the diagnosis of PIDs in childhood and for the accreditation of laboratories using Optilite® immunoturbidimetric reagents for IgG subclass measurement. Laboratory scientists and clinicians should be aware of the need for manufacturer-specific IgG subclass reference intervals.

HSV-1-Specific IgG Subclasses Distribution and Serum Neutralizing Activity in Alzheimer’s Disease and in Mild Cognitive Impairment

2018 ◽  
Vol 63 (1) ◽  
pp. 131-138 ◽  
Author(s):  
Simone Agostini ◽  
Roberta Mancuso ◽  
Ambra Hernis ◽  
Andrea Saul Costa ◽  
Raffaello Nemni ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cognitive Impairment ◽  
Mild Cognitive Impairment ◽  
Igg Subclasses ◽  
Neutralizing Activity ◽  
Specific Igg ◽  
Hsv 1

Caprine herpesvirus-1-specific IgG subclasses in naturally and experimentally infected goats

2009 ◽  
Vol 138 (3-4) ◽  
pp. 266-272 ◽  
Author(s):  
Mariarosaria Marinaro ◽  
Anna Lucia Bellacicco ◽  
Michele Camero ◽  
Elvira Tarsitano ◽  
Maria Tempesta ◽  
...  
Keyword(s):  
Igg Subclasses ◽  
Specific Igg ◽  
Herpesvirus 1
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