scholarly journals Multiple Antigen Peptide Containing B and T Cell Epitopes of F1 Antigen ofYersinia pestisShowed Enhanced Th1 Immune Response in Murine Model

2013 ◽  
Vol 77 (5) ◽  
pp. 361-371 ◽  
Author(s):  
R. Ali ◽  
R. A. Naqvi ◽  
S. Kumar ◽  
A. A. Bhat ◽  
D. N. Rao
Keyword(s):  
Immune Response ◽  
T Cell ◽  
Murine Model ◽  
T Cell Epitopes ◽  
Multiple Antigen ◽  
Antigen Peptide ◽  
Multiple Antigen Peptide ◽  
F1 Antigen ◽  
Th1 Immune Response

scholarly journals Immunogenicity of Well-Characterized Synthetic Plasmodium falciparum Multiple Antigen Peptide Conjugates

2001 ◽  
Vol 69 (8) ◽  
pp. 4884-4890 ◽  
Author(s):  
Manju B. Joshi ◽  
Albert A. Gam ◽  
Robert A. Boykins ◽  
Sanjai Kumar ◽  
John Sacci ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
T Cell ◽  
Solid Phase ◽  
Cell Epitope ◽  
T Cell Epitopes ◽  
Multiple Antigen ◽  
Antigen Peptide ◽  
Multiple Antigen Peptide ◽  
Different Strains

ABSTRACT Given the emerging difficulties with malaria drug resistance and vector control, as well as the persistent lack of an effective vaccine, new malaria vaccine development strategies are needed. We used a novel methodology to synthesize and fully characterize multiple antigen peptide (MAP) conjugates containing protective epitopes fromPlasmodium falciparum and evaluated their immunogenicity in four different strains of mice. A di-epitope MAP (T3-T1) containing two T-cell epitopes of liver stage antigen-1 (LSA-1), a di-epitope MAP containing T-cell epitopes from LSA-1 and from merozoite surface protein-1, and a tri-epitope MAP (T3-CS-T1) containing T3-T1 and a potent B-cell epitope from the circumsporozoite protein central repeat region were tested in this study. Mice of all four strains produced peptide-specific antibodies; however, the magnitude of the humoral response indicated strong genetic restriction between the different strains of mice. Anti-MAP antibodies recognized stage-specific proteins on the malaria parasites in an immunofluorescence assay. In addition, serum from hybrid BALB/cJ × A/J CAF1 mice that had been immunized with the tri-epitope MAP T3-CS-T1 successfully inhibited the malaria sporozoite invasion of hepatoma cells in vitro. Spleen cells from immunized mice also showed a genetically restricted cellular immune response when stimulated with the immunogen in vitro. This study indicates that well-characterized MAPs combining solid-phase synthesis and conjugation chemistries are potent immunogens and that this approach can be utilized for the development of subunit vaccines.

scholarly journals SARS-CoV-2 Human T cell Epitopes: adaptive immune response against COVID-19.

2021 ◽  
Author(s):  
Alba Grifoni ◽  
John Sidney ◽  
Randi Vita ◽  
Bjoern Peters ◽  
Shane Crotty ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cell ◽  
Adaptive Immune Response ◽  
T Cell Epitopes ◽  
Adaptive Immune ◽  
Human T Cell
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