Effects of meiotic inhibitors and gonadotrophins on porcine oocytes in vitro maturation, fertilization and development

2017 ◽  
Vol 52 (5) ◽  
pp. 873-880 ◽  
Author(s):  
MA Gil ◽  
A Nohalez ◽  
CA Martinez ◽  
JR Ake-Villanueva ◽  
F Centurion-Castro ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Meiotic Inhibitors

The effect of three dimensional prematuration culture with or without meiotic inhibitors on the in vitro maturation (IVM) outcome of bovine oocytes

2012 ◽  
Vol 98 (3) ◽  
pp. S23
Author(s):  
E.-A.M. Khalifa ◽  
T.A. Farghaly ◽  
S. Mostafa ◽  
M. Hussein ◽  
J. Goldfarb ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Three Dimensional ◽  
Meiotic Inhibitors ◽  
Bovine Oocytes

Synchronisation of canine germinal vesicle stage oocytes prior to in vitro maturation alters the kinetics of nuclear progression during subsequent resumption of meiosis

2008 ◽  
Vol 20 (5) ◽  
pp. 606 ◽  
Author(s):  
Carol Hanna ◽  
Suzanne Menges ◽  
Duane Kraemer ◽  
Charles R. Long
Keyword(s):  
In Vitro Maturation ◽  
Germinal Vesicle ◽  
Meiotic Arrest ◽  
Meiotic Inhibitors ◽  
Kinetics Of ◽  
Meiotic Competence ◽  
Effective Treatments

Inhibition of meiosis before in vitro maturation (IVM) can improve meiotic competence in immature mammalian oocytes. Therefore, meiosis-inhibiting agents were evaluated singularly for the ability to arrest and synchronise germinal vesicle (GV) stage canine oocytes, and the most effective treatments were combined to improve meiotic resumption rates. Oocytes cultured in 2 ng mL–1 oestradiol (E2), 10 IU mL–1 eCG, or both (EG) for 72 h resulted in significantly fewer oocytes resuming meiosis in EG than the control, E2, or with eCG. Oocytes cultured in 50 or 100 μmol L–1 of butyrolactone 1 or roscovitine (ROS) for up to 48 h did not resume meiosis nor increase subsequent meiotic resumption rates following IVM. A combination of 50 μmol L–1 ROS and EG treatment for 48 h significantly increased the proportion of canine oocytes in meiotic arrest. More importantly, following 48 h of IVM, ROS+EG-treated oocytes demonstrated a dramatic increase in the ability to resume meiosis compared with the non-treated controls (51.3 ± 8.2% and 10.8 ± 4.5%, respectively; P < 0.05). These data indicate that chemical and biological meiotic inhibitors are effective at inducing GV arrest in canine oocytes. Furthermore, these inhibitors are reversible and beneficial to subsequent meiotic resumption in vitro.

Effect of isoflavone compounds on the in vitro maturation of sheep oocytes

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
AA Amir ◽  
GB Martin ◽  
JM Kelly ◽  
DO Kleemann ◽  
Z Durmic ◽  
...  
Keyword(s):  
In Vitro Maturation

Glucocorticoid metabolism in bovine cumulus-oocyte complex during in vitro maturation

2014 ◽  
Author(s):  
Masafumi Tetsuka ◽  
Ryo Takagi ◽  
Nobuhiro Ambo ◽  
Yuta Zempo ◽  
Asuka Onuma
Keyword(s):  
In Vitro Maturation ◽  
Cumulus Oocyte Complex ◽  
Glucocorticoid Metabolism

Effect of vitrification on mitoсhondrial activity of oocytes during in vitro maturation

2019 ◽  
Vol 22 (4) ◽  
pp. 38-41
Author(s):  
T.I. Kuzmina ◽  
◽  
O.A. Epishko ◽  
E.S. Usenbekov ◽  
◽  
...  
Keyword(s):  
In Vitro Maturation
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