Glucocorticoid metabolism in bovine cumulus-oocyte complex during in vitro maturation

2014 ◽  
Author(s):  
Masafumi Tetsuka ◽  
Ryo Takagi ◽  
Nobuhiro Ambo ◽  
Yuta Zempo ◽  
Asuka Onuma
Keyword(s):  
In Vitro Maturation ◽  
Cumulus Oocyte Complex ◽  
Glucocorticoid Metabolism

Control of in vitro maturation of bovine cumulus-oocyte complex by preovulatory granulosa cells

1993 ◽  
Vol 34 (4) ◽  
pp. 431-442 ◽  
Author(s):  
Flora Rabahi ◽  
Danielle Monniaux ◽  
Claudine Pisselet ◽  
Philippe Durand
Keyword(s):  
Granulosa Cells ◽  
In Vitro Maturation ◽  
Cumulus Oocyte Complex

scholarly journals Glucocorticoid metabolism in the bovine cumulus–oocyte complex matured in vitro

Reproduction ◽  
2016 ◽  
Vol 151 (1) ◽  
pp. 73-82 ◽  
Author(s):  
Masafumi Tetsuka ◽  
Ryo Takagi ◽  
Nobuhiro Ambo ◽  
Thet Su Myat ◽  
Yuta Zempo ◽  
...  
Keyword(s):  
Subsequent Development ◽  
Cumulus Cells ◽  
Cumulus Oocyte Complex ◽  
Target Organs ◽  
Glucocorticoid Metabolism ◽  
Glucocorticoid Action ◽  
Expression And Activity

Glucocorticoid action in target organs is regulated by relative activities of 11β-HSD type 1 (HSD11B1) that mainly converts cortisone to active cortisol and type 2 (HSD11B2) that inactivates cortisol to cortisone. HSD11Bs have been shown to be expressed in the ovary of various species. However, little is known about the expression and activity of HSD11Bs in the bovine cumulus–oocyte complex (COC). In the present study, we investigated the expression and activities of HSD11Bs in in vitro-matured (IVM) bovine COCs. Bovine COCs were matured in M199 supplemented with or without FSH and FCS. The expression of HSD11B1 and HSD11B2 was measured by using quantitative RT-PCR in denuded oocytes (DO) and cumulus cells (CC). Reductive and oxidative activities of HSD11Bs were determined by radiometric conversion assay using labeled cortisol, cortisone or dexamethasone in intact COCs, DO or CC in the presence or absence of 11-keto-progesterone (11kP), a selective inhibitor of HSD11B2. The presence of HSD11Bs in the oocyte was examined by immunofluorescence microscopy. Oocytes exclusively expressed HSD11B2 and its expression and activity were largely unchanged during IVM. CC, on the other hand, exclusively expressed HSD11B1 and its expression and activity were upregulated as IVM progressed. As a result, the net glucocorticoid metabolism shifted from inactivation to activation towards the end of IVM. These results indicate that the bovine COC is capable of modulating local glucocorticoid concentration and, by doing so, may create an environment that is favorable to ovulating oocyte for maturation, fertilization and subsequent development.

scholarly journals Energy metabolism of the equine cumulus oocyte complex during in vitro maturation

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
N. Lewis ◽  
K. Hinrichs ◽  
H. J. Leese ◽  
C. McG. Argo ◽  
D. R. Brison ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
In Vitro Maturation ◽  
Cumulus Oocyte Complex
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