Functional characterization of terpene synthases and chemotypic variation in three lavender species of section Stoechas

2014 ◽  
Vol 153 (1) ◽  
pp. 43-57 ◽  
Author(s):  
Tarek Benabdelkader ◽  
Yann Guitton ◽  
Bernard Pasquier ◽  
Jean Louis Magnard ◽  
Frédéric Jullien ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Terpene Synthases

Combining transcriptomics-based and proteomics-based approaches for functional characterization of terpene synthases of “Arnica da Serra” (Asteraceae)

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
DP Pavarini ◽  
LP Oliveira ◽  
LM Pereira ◽  
AP Natsui ◽  
O Spring ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Terpene Synthases

Genome-wide analysis of terpene synthases in soybean: functional characterization of GmTPS3

Gene ◽  
2014 ◽  
Vol 544 (1) ◽  
pp. 83-92 ◽  
Author(s):  
Jianyu Liu ◽  
Fang Huang ◽  
Xia Wang ◽  
Man Zhang ◽  
Rui Zheng ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Terpene Synthases ◽  
Genome Wide Analysis ◽  
Genome Wide

scholarly journals Cloning and functional characterization of three terpene synthases from lavender (Lavandula angustifolia)

2007 ◽  
Vol 465 (2) ◽  
pp. 417-429 ◽  
Author(s):  
Christian Landmann ◽  
Barbara Fink ◽  
Maria Festner ◽  
Márta Dregus ◽  
Karl-Heinz Engel ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Lavandula Angustifolia ◽  
Terpene Synthases

Identification and characterization of a kunzeaol synthase from Thapsia garganica: implications for the biosynthesis of the pharmaceutical thapsigargin

2012 ◽  
Vol 448 (2) ◽  
pp. 261-271 ◽  
Author(s):  
Benjamin Pickel ◽  
Damian P. Drew ◽  
Tom Manczak ◽  
Corinna Weitzel ◽  
Henrik T. Simonsen ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Antagonistic Effect ◽  
Farnesyl Diphosphate ◽  
Fragmentation Pattern ◽  
Terpene Synthases ◽  
Engineered Yeast ◽  
Thapsia Garganica ◽  
Identification And Characterization

Thapsigargin is a major terpenoid constituent of Thapsia garganica root. Owing to its potent antagonistic effect on the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase, thapsigargin has been widely used to study Ca2+ signalling and is also a potential drug for prostate cancer. Despite its importance, thapsigargin biosynthesis in T. garganica remains unknown. In order to decipher thapsigargin biosynthesis, deep transcript sequencing (454 and Illumina) of the T. garganica root was performed, and two terpene synthases (TgTPS1/2) were identified. Functional characterization of their encoded enzymes in a metabolically engineered yeast revealed that TgTPS1 synthesized δ-cadinene, whereas TgTPS2 produced ten distinct terpenoids. However, cultivation of the TgTPS2-expressing yeast in pH-maintained conditions (pH 6–7) yielded one major oxygenated sesquiterpenoid, suggesting that formation of multiple terpenoids was caused by acidity. The major terpene product from TgTPS2 was identified as 6β-hydroxygermacra-1(10),4-diene (kunzeaol) by mass-fragmentation pattern, retention index, the nature of its acid-induced degradation and NMR. Also, recombinant TgTPS2 efficiently catalysed the synthesis of kunzeaol in vitro from farnesyl diphosphate with a Km of 2.6 μM and a kcat of 0.03 s−1. The present paper is the first report of a kunzeaol synthase, and a mechanism for the transformation of kunzeaol into the thapsigargin backbone is proposed.

scholarly journals Rapid Discovery and Functional Characterization of Terpene Synthases from Four Endophytic Xylariaceae

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0146983 ◽  
Author(s):  
Weihua Wu ◽  
William Tran ◽  
Craig A. Taatjes ◽  
Jorge Alonso-Gutierrez ◽  
Taek Soon Lee ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Terpene Synthases

Isolation and functional characterization of four microbial type terpene synthases from ferns

2020 ◽  
Vol 155 ◽  
pp. 716-724
Author(s):  
Piao-Yi Wang ◽  
Rong Ni ◽  
Ting-Ting Zhu ◽  
Chun-Jing Sun ◽  
Hong-Xiang Lou ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Terpene Synthases
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