Integrated proteome and lipidome analysis of naturally aged safflower seeds varying in vitality

Plant Biology ◽  
2021 ◽  
Author(s):  
C. Chen ◽  
R. Wang ◽  
S. Dong ◽  
J. Wang ◽  
C‐X. Ren ◽  
...  
Keyword(s):  
Lipidome Analysis

scholarly journals Multi-Omic Single-Shot Technology for Integrated Proteome and Lipidome Analysis

2021 ◽  
Vol 93 (9) ◽  
pp. 4217-4222 ◽  
Author(s):  
Yuchen He ◽  
Edrees H. Rashan ◽  
Vanessa Linke ◽  
Evgenia Shishkova ◽  
Alexander S. Hebert ◽  
...  
Keyword(s):  
Single Shot ◽  
Lipidome Analysis

scholarly journals Lipidome analysis of milk composition in humans, monkeys, bovids, and pigs

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Aleksandra Mitina ◽  
Pavel Mazin ◽  
Anna Vanyushkina ◽  
Nikolay Anikanov ◽  
Waltraud Mair ◽  
...  
Keyword(s):  
Milk Composition ◽  
Lipidome Analysis

Characterization of lipid accumulation and lipidome analysis in the oleaginous yeasts Rhodosporidium azoricum and Trichosporon oleaginosus

2017 ◽  
Vol 238 ◽  
pp. 281-289 ◽  
Author(s):  
Claudia Capusoni ◽  
Valentina Rodighiero ◽  
Daniela Cucchetti ◽  
Silvia Galafassi ◽  
Daniele Bianchi ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Oleaginous Yeasts ◽  
Lipidome Analysis

scholarly journals Sterols as dietary markers for Drosophila melanogaster

2019 ◽  
Author(s):  
Oskar Knittelfelder ◽  
Elodie Prince ◽  
Susanne Sales ◽  
Eric Fritzsche ◽  
Thomas Wöhner ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Cold Acclimation ◽  
Lipid Membranes ◽  
Population Based ◽  
Sterol Composition ◽  
Molar Ratio ◽  
Shotgun Lipidomics ◽  
Organ Specific ◽  
In The Wild ◽  
Lipidome Analysis

AbstractDuring cold acclimation fruit flies switch their feeding from yeast to plant food, however there are no robust markers to monitor it in the wild. Drosophila melanogaster is a sterol auxotroph and relies on dietary sterols to produce lipid membranes, lipoproteins and molting hormones. We employed shotgun lipidomics to quantify eight major food sterols in total extracts of heads, female and male genital tracts of adult flies. We found that their sterol composition is dynamic and reflective of flies diet in an organ-specific manner. Season-dependent changes observed in the organs of wild-living flies suggested that the molar ratio between yeast (ergosterol, zymosterol) and plant (sitosterol, stigmasterol) sterols is a quantifiable, generic and unequivocal marker of their feeding behavior, including cold acclimation. It provides technically simpler and more contrast readout compared to the full lipidome analysis and is suitable for ecological and environmental population-based studies.

scholarly journals Male sterile 305 Mutation Leads the Misregulation of Anther Cuticle Formation by Disrupting Lipid Metabolism in Maize

2020 ◽  
Vol 21 (7) ◽  
pp. 2500
Author(s):  
Haichun Shi ◽  
Yang Yu ◽  
Ronghuan Gu ◽  
Chenxi Feng ◽  
Yu Fu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Genetic Material ◽  
Lipid Synthesis ◽  
Gas Chromatography Mass Spectrometry ◽  
Male Sterile ◽  
Anther Cuticle ◽  
Phenylpropanoid Biosynthesis ◽  
Total Fatty Acids ◽  
Physical Barriers ◽  
Lipidome Analysis

The anther cuticle, which is mainly composed of lipid polymers, functions as physical barriers to protect genetic material intact; however, the mechanism of lipid biosynthesis in maize (Zea mays. L.) anther remains unclear. Herein, we report a male sterile mutant, male sterile 305 (ms305), in maize. It was shown that the mutant displayed a defective anther tapetum development and premature microspore degradation. Three pathways that are associated with the development of male sterile, including phenylpropanoid biosynthesis, biosynthesis of secondary metabolites, as well as cutin, suberine, and wax biosynthesis, were identified by transcriptome analysis. Gas chromatography-mass spectrometry disclosed that the content of cutin in ms305 anther was significantly lower than that of fertile siblings during the abortion stage, so did the total fatty acids, which indicated that ms305 mutation might lead to blocked synthesis of cutin and fatty acids in anther. Lipidome analysis uncovered that the content of phosphatidylcholine, phosphatidylserine, diacylglycerol, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol in ms305 anther was significantly lower when compared with its fertile siblings, which suggested that ms305 mutation disrupted lipid synthesis. In conclusion, our findings indicated that ms305 might affect anther cuticle and microspore development by regulating the temporal progression of the lipidome in maize.

Recent advances in microscale separation techniques for lipidome analysis

The Analyst ◽  
2021 ◽  
Author(s):  
Takayuki Kawai ◽  
Nobuaki Matsumori ◽  
Koji Otsuka
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
Liquid Phase ◽  
Review Paper ◽  
Capillary Liquid Chromatography ◽  
Separation Techniques ◽  
Recent Advances ◽  
Lipidome Analysis ◽  
Capillary Liquid

This review paper highlights the recent research on liquid-phase microscale separation techniques for lipidome analysis over the last 10 years, focusing on capillary liquid chromatography and capillary electrophoresis coupled with mass spectrometry.

Biomarkers predictive of long-term fertility found in vaginal lipidome of gilts at weaning

2021 ◽  
Author(s):  
Kayla M Mills ◽  
Christina R Ferreira ◽  
Jebadiah G Stevens ◽  
Kara R Stewart ◽  
Theresa M Casey
Keyword(s):  
Reproductive Performance ◽  
Multiple Reaction Monitoring ◽  
Production Costs ◽  
Future Research ◽  
Reproductive Efficiency ◽  
High Fertility ◽  
Vaginal Swabs ◽  
Fertility Potential ◽  
Lipidome Analysis

Abstract A marker indicative of fertility potential of replacement gilts early in development would decrease culling rates in the sow herd, improve sow herd reproductive efficiency, and reduce production costs. The objective of this study was to determine if vaginal lipid profiles at 21 d postnatal (PN) could predict sow reproductive performance. Vaginal swabs of the anterior vagina were taken at 21 ± 4 d PN from gilts born on a commercial sow production facility for lipidomic analysis. Animals were followed prospectively for two years and assigned to reproductive performance categories based on observation of estrus or piglets weaned per sow per year (PSY) across two farrowings. Lipids were extracted from cellular material collected with swabs taken from high fertility (HF; n=28; ≥26 PSY) and infertile (IF; n=34; no estrus, no pregnancy) animals and multiple reaction monitoring (MRM)-profiling was used for lipidome analysis. Relative abundance of arachidonic acid (ARA, C20:4) and docosahexaenoic acid (DHA, C22:6) were lower (P<0.05) in IF gilts than HF gilts, whereas abundance of the free fatty acids cerotic (C26:0), ximenic (C26:1), and nonadecanoic (C19:0) acids were greater (P<0.05) in IF gilts. Additionally, eicosapentaenoic acid (C20:5) a precursor of prostaglandins was higher (P<0.05) in IF gilts. The perspective of having a panel of lipids captured with vaginal swabs at weaning that can predict reproductive efficiency of gilts shows promise and warrants future research in this area.

Comprehensive Lipidome Analysis by Shotgun Lipidomics on a Hybrid Quadrupole-Orbitrap-Linear Ion Trap Mass Spectrometer

2014 ◽  
Vol 26 (1) ◽  
pp. 133-148 ◽  
Author(s):  
Reinaldo Almeida ◽  
Josch Konstantin Pauling ◽  
Elena Sokol ◽  
Hans Kristian Hannibal-Bach ◽  
Christer S. Ejsing
Keyword(s):  
Mass Spectrometer ◽  
Ion Trap ◽  
Linear Ion Trap ◽  
Shotgun Lipidomics ◽  
Ion Trap Mass Spectrometer ◽  
Lipidome Analysis
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