scholarly journals Male sterile 305 Mutation Leads the Misregulation of Anther Cuticle Formation by Disrupting Lipid Metabolism in Maize

2020 ◽  
Vol 21 (7) ◽  
pp. 2500
Author(s):  
Haichun Shi ◽  
Yang Yu ◽  
Ronghuan Gu ◽  
Chenxi Feng ◽  
Yu Fu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Genetic Material ◽  
Lipid Synthesis ◽  
Gas Chromatography Mass Spectrometry ◽  
Male Sterile ◽  
Anther Cuticle ◽  
Phenylpropanoid Biosynthesis ◽  
Total Fatty Acids ◽  
Physical Barriers ◽  
Lipidome Analysis

The anther cuticle, which is mainly composed of lipid polymers, functions as physical barriers to protect genetic material intact; however, the mechanism of lipid biosynthesis in maize (Zea mays. L.) anther remains unclear. Herein, we report a male sterile mutant, male sterile 305 (ms305), in maize. It was shown that the mutant displayed a defective anther tapetum development and premature microspore degradation. Three pathways that are associated with the development of male sterile, including phenylpropanoid biosynthesis, biosynthesis of secondary metabolites, as well as cutin, suberine, and wax biosynthesis, were identified by transcriptome analysis. Gas chromatography-mass spectrometry disclosed that the content of cutin in ms305 anther was significantly lower than that of fertile siblings during the abortion stage, so did the total fatty acids, which indicated that ms305 mutation might lead to blocked synthesis of cutin and fatty acids in anther. Lipidome analysis uncovered that the content of phosphatidylcholine, phosphatidylserine, diacylglycerol, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol in ms305 anther was significantly lower when compared with its fertile siblings, which suggested that ms305 mutation disrupted lipid synthesis. In conclusion, our findings indicated that ms305 might affect anther cuticle and microspore development by regulating the temporal progression of the lipidome in maize.

scholarly journals Analysis Fatty Acids Profile in Tabanus bivittatus Mats with Gas Chromatography-Mass Spectrometry

2015 ◽  
Vol 9 (1) ◽  
pp. 113-118
Author(s):  
Wang Yanhua ◽  
Wu Fuhua ◽  
Guo Zhaohan ◽  
Peng Mingxing ◽  
Xia Min ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Palmitoleic Acid ◽  
Unsaturated Fatty Acids ◽  
Methyl Esters ◽  
Soxhlet Extraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Fatty Acids Profile ◽  
Total Fatty Acids

Tabanus bivittatus Mats., a traditional Chinese medicine, is commonly used for cardiovascular disorders treatment including atherosclerosis. There have been only a few researches on its chemical components, and no detailed report has appeared on its fatty acids. To develop a simple and effective method for the extraction of total fatty acids from Tabanus bivittatus Mats., the Soxhlet extraction (SE) condition was optimized with response surface methodology. The fatty acid composition of the extract were determined by GC-MS with previous derivatization to fatty acid methyl esters (FAMEs). The major fatty acids in Tabanus bivittatus Mats. were oleic acid, palmitic acid, linoleic acid, palmitoleic acid, and stearic acid, and the unsaturated fatty acids occupy 63.9% of the total fatty acids.

(omega-1)-Hydroxymonocarboxylic acids in urine of infants fed medium-chain triglycerides.

1981 ◽  
Vol 27 (10) ◽  
pp. 1661-1664 ◽  
Author(s):  
Y Shigematsu ◽  
T Momoi ◽  
M Sudo ◽  
Y Suzuki
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Octanoic Acid ◽  
Monocarboxylic Acid ◽  
Decanoic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Medium Chain Triglycerides ◽  
Medium Chain ◽  
Chain Acyl ◽  
Total Fatty Acids

Abstract Organic acids in the urine of infants fed a formula containing medium-chain triglycerides (octanoic acid 49% and decanoic acid 26% of total fatty acids) were determined by gas chromatography-mass spectrometry. We found a considerable amount of 5-hydroxyhexanoic acid and 7-hydroxyoctanoic acid, but no detectable 9-hydroxydecanoic acid, as well as a large amount of C6-C10-dicarboxylic acid. We believe that such acids are derived, via (omega-1)-hydroxylation, from medium-chain monocarboxylic acid or medium-chain acyl-CoA in the liver cell. The degree of (omega-1)-hydroxylation of medium-chain fatty acids relative to omega-hydroxylation apparently increases as the chain length of the fatty acids decreases.

THE LIPIDS OF LIPOMYCES LIPOFER

1967 ◽  
Vol 45 (2) ◽  
pp. 171-178 ◽  
Author(s):  
Frances A. McElroy ◽  
H. B. Stewart
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Neutral Lipids ◽  
Lipid Synthesis ◽  
Triglyceride Fraction ◽  
Principal Fatty Acid ◽  
High Lipid ◽  
Total Fatty Acids ◽  
Lipomyces Lipofer ◽  
Chromatographic Evidence

As a preliminary to the study of lipid synthesis in Lipomyces lipofer, the nature of the lipid accumulated by this organism was investigated. Neutral lipids were found to predominate, especially in older cultures of high lipid content. The triglyceride fraction was the major component, with smaller quantities of hydrocarbon, free and esterified ergosterol, monoglyceride, diglyceride, and free fatty acid also present. Phosphatid-y-linositol constituted almost one-quarter of the phospholipid fraction. The phosphoglycerides of choline, serine and ethanolamine were also detected. Gas chromatographic evidence indicated that 16:0, 16:1, 18:0, 18:1, 18:2, 18:3 were the principal fatty acid components. The 18:1 fraction was shown by oxidative-cleavage studies to be primarily oleic acid. This fraction predominated, reaching a level of 69% of the total fatty acids in older cultures.

scholarly journals Enhancement of gamma-linolenic acid production by the fungus Mucor sp LB-54 by growth temperature

1999 ◽  
Vol 30 (2) ◽  
pp. 170-176 ◽  
Author(s):  
Patrícia de Oliveira Carvalho ◽  
Joaquim Gilberto de Oliveira ◽  
Gláucia Maria Pastore
Keyword(s):  
Fatty Acids ◽  
Growth Temperature ◽  
Lipid Production ◽  
Basal Medium ◽  
Culture Conditions ◽  
Gas Chromatography Mass Spectrometry ◽  
Glucose Addition ◽  
Dry Cell Weight ◽  
Total Fatty Acids ◽  
Maximal Production

As a relatively prolific producer of GLA, the strain of Mucor sp LB-54 was selected for a study at different growth temperatures in shaker flask culture. The strain used in our experiment was capable to accumulate a relatively high amount of intracellular lipid, 20.73 % of dry cell weight, and GLA content of 15 % of total fatty acids after 5 days of incubation at 28°C. As the growth temperature was decreased from 28 to 12°C the percentage of GLA increased from 15 to 24 % of total fatty acids. In order to optimize the culture conditions for rapid biomass production and lipid production with a high proportion of GLA, the fungus was grown at two temperature combinations associated supplies of carbon source (glucose) in the culture medium. Maximal production of GLA (74 mg/l) was obtained from the Mucor sp LB-54 strain after 5 days of incubation at 28°C in basal medium following glucose addition (7 % w/v) and incubation for an additional 3 days at 12°C. The identity of GLA found in the strain of Mucor sp LB-54 was confirmed by the coupled gas chromatography-mass spectrometry

scholarly journals Seaweed Blends as a Valuable Source of Polyunsaturated and Healthy Fats for Nutritional and Food Applications

Marine Drugs ◽  
2021 ◽  
Vol 19 (12) ◽  
pp. 684
Author(s):  
Francisca Marques ◽  
Diana Lopes ◽  
Elisabete da Costa ◽  
Tiago Conde ◽  
Andreia Rego ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Healthy Lifestyle ◽  
Gas Chromatography Mass Spectrometry ◽  
Food Markets ◽  
Sustainable Food ◽  
Lipid Fractions ◽  
Total Fatty Acids ◽  
Food Applications ◽  
Total Carbohydrates ◽  
Functional Features

Seaweeds are considered healthy and sustainable food. Although their consumption is modest in Western countries, the demand for seaweed in food markets is increasing in Europe. Each seaweed species has unique nutritional and functional features. The preparation of blends, obtained by mixing several seaweeds species, allows the obtaining of maximum benefits and ingredients with single characteristics. In this work, five seaweed blends, commercially available and produced under organic conditions in Europe, were characterized. The proximal composition included contents of ash (20.28–28.68% DW), proteins (17.79–26.61% DW), lipids (0.55–1.50% DW), and total carbohydrates (39.47–47.37% DW). Fatty acid profiles were determined by gas chromatography–mass spectrometry (GC–MS), allowing quantification of healthy fatty acids, namely n-3 and n-6 polyunsaturated fatty acids (PUFA), and calculation of lipid quality indices. Each blend showed a characteristic PUFA content in the lipid pool (35.77–49.43% of total fatty acids) and the content in essential and healthy n-3 PUFA is highlighted. The atherogenicity (0.54–0.72) and thrombogenicity (0.23–0.45) indices evidenced a good nutritional value of lipid fractions. As nutritional and environmentally attractive products, the consumption of the studied seaweed blends can contribute to a healthy lifestyle.

Determination of total fatty acids (C8–C22) in sludges by gas chromatography–mass spectrometry

1998 ◽  
Vol 826 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Antonio González Casado ◽  
Enrique J Alonso Hernández ◽  
Pedro Espinosa ◽  
Jose Luis Vı́lchez
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Total Fatty Acids

scholarly journals Anaerobic 1-Alkene Metabolism by the Alkane- and Alkene-Degrading Sulfate Reducer Desulfatibacillum aliphaticivorans Strain CV2803T

2007 ◽  
Vol 73 (24) ◽  
pp. 7882-7890 ◽  
Author(s):  
Vincent Grossi ◽  
Cristiana Cravo-Laureau ◽  
Alain Méou ◽  
Danielle Raphel ◽  
Frédéric Garzino ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Inorganic Carbon ◽  
Direct Evidence ◽  
Gas Chromatography Mass Spectrometry ◽  
Sulfate Reducing ◽  
Branched Fatty Acids ◽  
Initial Activation ◽  
Fumarate Addition ◽  
Chain Lengths ◽  
Methyl Branched Fatty Acids

ABSTRACT The alkane- and alkene-degrading, marine sulfate-reducing bacterium Desulfatibacillum aliphaticivorans strain CV2803T, known to oxidize n-alkanes anaerobically by fumarate addition at C-2, was investigated for its 1-alkene metabolism. The total cellular fatty acids of this strain were predominantly C-(even number) (C-even) when it was grown on C-even 1-alkenes and predominantly C-(odd number) (C-odd) when it was grown on C-odd 1-alkenes. Detailed analyses of those fatty acids by gas chromatography-mass spectrometry after 6- to 10-week incubations allowed the identification of saturated 2- and 4-ethyl-, 2- and 4-methyl-, and monounsaturated 4-methyl-branched fatty acids with chain lengths that correlated with those of the 1-alkene. The growth of D. aliphaticivorans on (per)deuterated 1-alkenes provided direct evidence of the anaerobic transformation of these alkenes into the corresponding 1-alcohols and into linear as well as 10- and 4-methyl-branched fatty acids. Experiments performed with [13C]bicarbonate indicated that the initial activation of 1-alkene by the addition of inorganic carbon does not occur. These results demonstrate that D. aliphaticivorans metabolizes 1-alkene by the oxidation of the double bond at C-1 and by the subterminal addition of organic carbon at both ends of the molecule [C-2 and C-(ω-1)]. The detection of ethyl-branched fatty acids from unlabeled 1-alkenes further suggests that carbon addition also occurs at C-3. Alkylsuccinates were not observed as potential initial intermediates in alkene metabolism. Based on our observations, the first pathways for anaerobic 1-alkene metabolism in an anaerobic bacterium are proposed. Those pathways indicate that diverse initial reactions of 1-alkene activation can occur simultaneously in the same strain of sulfate-reducing bacterium.

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