Universal stress protein‐like gene from mulberry enhances abiotic stress tolerance in Escherichia coli and transgenic tobacco cells

Plant Biology ◽  
2021 ◽  
Author(s):  
K. H. Dhanyalakshmi ◽  
K. N. Nataraja
Keyword(s):  
Escherichia Coli ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stress Tolerance ◽  
Stress Protein ◽  
Tobacco Cells ◽  
Universal Stress Protein

scholarly journals CLONING AND EXPRESSION OF UNIVERSAL STRESS PROTEIN 2 (USP2) GENE IN ESCHERICHIA COLI

2021 ◽  
Vol 2021 (1) ◽  
Author(s):  
A Akram ◽  
K Arshad ◽  
MN Hafeez
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Protein Gene ◽  
Abiotic Stress Tolerance ◽  
Stress Protein ◽  
Crop Plants ◽  
Cloning And Expression ◽  
Major Type ◽  
Transgenic Crop ◽  
Universal Stress Protein

Different types of abiotic stresses inhibit the normal growth of plants by changing their physical biochemical, morphological, and molecular traits. It links to the polygenic traits, which is controlled with the help of different genes, due to this polygenetic the manipulation of foreign genetic makeup is very difficult. Drought stress is the very major type of threat to reduce the yield of cash crops in Pakistan and as well as in all over the world. Gene manipulation is the solution to face this problem by producing genetically modified crop plants that have the ability to survive in drought conditions. Universal stress protein gene has been already identified in bacteria which showed its response under stressed conditions, by manipulation of universal stress protein gene. It was found from our study that the bacterial cells transformed with the USP2 gene isolated from cotton induced abiotic stress tolerance under heat, osmotic, and salt stress. It was suggested from our findings that the USP2 gene could be used to produce abiotic stress tolerance transgenic crop plants to enhance crop plant yield and quality.

Ectopic expression of a fruit phytoene synthase from Citrus paradisi Macf. promotes abiotic stress tolerance in transgenic tobacco

2012 ◽  
Vol 39 (12) ◽  
pp. 10201-10209 ◽  
Author(s):  
Luciana C. Cidade ◽  
Tahise M. de Oliveira ◽  
Amanda F. S. Mendes ◽  
Amanda F. Macedo ◽  
Eny I. S. Floh ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stress Tolerance ◽  
Ectopic Expression ◽  
Phytoene Synthase ◽  
Citrus Paradisi

scholarly journals The SbMT-2 Gene from a Halophyte Confers Abiotic Stress Tolerance and Modulates ROS Scavenging in Transgenic Tobacco

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e111379 ◽  
Author(s):  
Amit Kumar Chaturvedi ◽  
Manish Kumar Patel ◽  
Avinash Mishra ◽  
Vivekanand Tiwari ◽  
Bhavanath Jha
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stress Tolerance ◽  
Ros Scavenging

scholarly journals OsPOP5, A Prolyl Oligopeptidase Family Gene from Rice Confers Abiotic Stress Tolerance in Escherichia coli

2013 ◽  
Vol 14 (10) ◽  
pp. 20204-20219 ◽  
Author(s):  
Cun-Mei Tan ◽  
Rong-Jun Chen ◽  
Jian-Hua Zhang ◽  
Xiao-Ling Gao ◽  
Li-Hua Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Prolyl Oligopeptidase ◽  
Family Gene

scholarly journals TheSsDREBTranscription Factor from the Succulent HalophyteSuaeda salsaEnhances Abiotic Stress Tolerance in Transgenic Tobacco

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Xu Zhang ◽  
Xiaoxue Liu ◽  
Lei Wu ◽  
Guihong Yu ◽  
Xiue Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stress Tolerance ◽  
Soluble Sugars ◽  
Sequence Alignments ◽  
Multiple Sequence ◽  
Dreb Transcription Factor

Dehydration-responsive element-binding (DREB) transcription factor (TF) plays a key role for abiotic stress tolerance in plants. In this study, a novel cDNA encoding DREB transcription factor, designatedSsDREB, was isolated from succulent halophyteSuaeda salsa. This protein was classified in the A-6 group of DREB subfamily based on multiple sequence alignments and phylogenetic characterization. Yeast one-hybrid assays showed that SsDREB protein specifically binds to the DRE sequence and could activate the expression of reporter genes in yeast, suggesting that the SsDREB protein was a CBF/DREB transcription factor. Real-time RT-PCR showed thatSsDREBwas significantly induced under salinity and drought stress. Overexpression ofSsDREBcDNA in transgenic tobacco plants exhibited an improved salt and drought stress tolerance in comparison to the nontransformed controls. The transgenic plants revealed better growth, higher chlorophyll content, and net photosynthesis rate, as well as higher level of proline and soluble sugars. The semiquantitative PCR of transgenics showed higher expression of stress-responsive genes. These data suggest that theSsDREBtranscription factor is involved in the regulation of salt stress tolerance in tobacco by the activation of different downstream gene expression.

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