scholarly journals Expression of a Bifunctional Fusion of the Escherichia coli Genes for Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase in Transgenic Rice Plants Increases Trehalose Accumulation and Abiotic Stress Tolerance without Stunting Growth

2003 ◽  
Vol 131 (2) ◽  
pp. 516-524 ◽  
Author(s):  
In-Cheol Jang ◽  
Se-Jun Oh ◽  
Ju-Seok Seo ◽  
Won-Bin Choi ◽  
Sang Ik Song ◽  
...  
Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 589 ◽  
Author(s):  
Choi ◽  
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Melatonin 2-hydroxylase (M2H) catalyzes the conversion of melatonin into 2hydroxymelatonin (2OHM), which is present in plants at a higher concentration than melatonin. Although M2H has been cloned, the in vivo function of its product is unknown. Here, we generated stable T2 homozygous transgenic rice plants in which expression of endogenous M2H was suppressed (RNAi lines). However, we failed to generate M2H overexpression transgenic rice due to failure of somatic embryogenesis. The M2H transcript level showed a diurnal rhythm with a peak at night concomitantly with the peak concentration of 2OHM. RNAi rice showed a reduced M2H mRNA level and 2OHM and melatonin concentrations. The unexpected decrease in the melatonin concentration was caused by redirection of melatonin into cyclic 3hydroxymelatonin via a detour catabolic pathway. Thus, the decrease in the melatonin concentration in M2H RNAi rice led to slowed seedling growth and delayed germination. By contrast, the transient increase in the melatonin concentration was of greater magnitude in the M2H RNAi than the wild-type rice upon cadmium treatment due to possible suppression of melatonin degradation. Due to its higher concentration of melatonin, the M2H RNAi rice displayed tolerance to senescence, salt, and tunicamycin stresses. Therefore, the increase in the melatonin concentration caused by suppression of melatonin degradation or by overexpression of melatonin biosynthetic genes enhances stress tolerance in rice.


2013 ◽  
Vol 14 (10) ◽  
pp. 20204-20219 ◽  
Author(s):  
Cun-Mei Tan ◽  
Rong-Jun Chen ◽  
Jian-Hua Zhang ◽  
Xiao-Ling Gao ◽  
Li-Hua Li ◽  
...  

1999 ◽  
Vol 2 (3) ◽  
pp. 172-177 ◽  
Author(s):  
Kiyomi Ono ◽  
Ken Ishimaru ◽  
Naohiro Aoki ◽  
Sakiko Takahashi ◽  
Kenjiroh Ozawa ◽  
...  

2010 ◽  
Vol 150 ◽  
pp. 115-115
Author(s):  
J.S. Jeong ◽  
Y.S. Kim Kim ◽  
Y.D. Choi ◽  
M.K. Kim ◽  
J.K. Kim

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