scholarly journals Long-term cadmium exposure influences the abundance of proteins that impact the cell wall structure in Medicago sativa stems

Plant Biology ◽  
2018 ◽  
Vol 20 (6) ◽  
pp. 1023-1035 ◽  
Author(s):  
A. Gutsch ◽  
E. Keunen ◽  
G. Guerriero ◽  
J. Renaut ◽  
A. Cuypers ◽  
...  
Keyword(s):  
Cell Wall ◽  
Medicago Sativa ◽  
Cadmium Exposure ◽  
Cell Wall Structure ◽  
Wall Structure

scholarly journals Changes in the Proteome of Medicago sativa Leaves in Response to Long-Term Cadmium Exposure Using a Cell-Wall Targeted Approach

2018 ◽  
Vol 19 (9) ◽  
pp. 2498 ◽  
Author(s):  
Annelie Gutsch ◽  
Salha Zouaghi ◽  
Jenny Renaut ◽  
Ann Cuypers ◽  
Jean-Francois Hausman ◽  
...  
Keyword(s):  
Cell Wall ◽  
Medicago Sativa ◽  
Protein Identification ◽  
Molecular Mechanisms ◽  
Time Of Flight ◽  
Cell Wall Protein ◽  
Wall Structure ◽  
Cd Exposure ◽  
Targeted Approach

Accumulation of cadmium (Cd) shows a serious problem for the environment and poses a threat to plants. Plants employing various cellular and molecular mechanisms to limit Cd toxicity and alterations of the cell wall structure were observed upon Cd exposure. This study focuses on changes in the cell wall protein-enriched subproteome of alfalfa (Medicago sativa) leaves during long-term Cd exposure. Plants grew on Cd-contaminated soil (10 mg/kg dry weight (DW)) for an entire season. A targeted approach was used to sequentially extract cell wall protein-enriched fractions from the leaves and quantitative analyses were conducted with two-dimensional difference gel electrophoresis (2D DIGE) followed by protein identification with matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time of flight (TOF/TOF) mass spectrometry. In 212 spots that showed a significant change in intensity upon Cd exposure a single protein was identified. Of these, 163 proteins are predicted to be secreted and involved in various physiological processes. Proteins of other subcellular localization were mainly chloroplastic and decreased in response to Cd, which confirms the Cd-induced disturbance of the photosynthesis. The observed changes indicate an active defence response against a Cd-induced oxidative burst and a restructuring of the cell wall, which is, however, different to what is observed in M. sativa stems and will be discussed.

scholarly journals Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Annelie Gutsch ◽  
Kjell Sergeant ◽  
Els Keunen ◽  
Els Prinsen ◽  
Gea Guerriero ◽  
...  
Keyword(s):  
Cell Wall ◽  
Medicago Sativa ◽  
Cadmium Exposure ◽  
Pectic Polysaccharides

Chemical Composition, Morphological Characteristics, and Cell Wall Structure of Malaysian Oil Palm Fibers

2008 ◽  
Vol 47 (3) ◽  
pp. 273-280 ◽  
Author(s):  
H. P. S. Abdul Khalil ◽  
M. Siti Alwani ◽  
R. Ridzuan ◽  
H. Kamarudin ◽  
A. Khairul
Keyword(s):  
Cell Wall ◽  
Chemical Composition ◽  
Oil Palm ◽  
Morphological Characteristics ◽  
Cell Wall Structure ◽  
Wall Structure
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