Analyses of several seed viability markers in individual recalcitrant seeds ofEugenia stipitataMcVaugh with totipotent germination

Plant Biology ◽  
2016 ◽  
Vol 19 (1) ◽  
pp. 6-13 ◽  
Author(s):  
G. P. Calvi ◽  
F. F. Aud ◽  
I. D. K. Ferraz ◽  
H. W. Pritchard ◽  
I. Kranner
Keyword(s):  
Seed Viability ◽  
Recalcitrant Seeds ◽  
Viability Markers

scholarly journals The effects of kencur (Kaempferia galanga L.) extract and storage period on seed borne fungi and citrus (Citrus sp.) seed viability

2006 ◽  
Vol 4 (2) ◽  
pp. 79-84
Author(s):  
DEWI DEVY DAYUNI ◽  
WARSOKO WIRYOWIDODO ◽  
SALIM WIDONO
Keyword(s):  
Block Design ◽  
Seed Viability ◽  
Storage Period ◽  
Moisture Level ◽  
Recalcitrant Seeds ◽  
High Moisture ◽  
Significance Level ◽  
High Moisture Level ◽  
Citrus Seed ◽  
Citrus Seeds

The recalcitrant seeds need a high moisture level during in storage to prevent loss its viability. The storage at a high moisture level is a favorable condition for fungi growth and on the other hand, a low seeds moisture level will loss the seeds viability. The citrus seeds as one of recalcitrant seeds need a special condition in storage. The storage of citrus seeds by using kencur (Kaempferia galangal L.) extract can protect the seeds against fungi during storage and preserve its viability. The purpose of this research was to determine the best concentration of K. galanga extract which protects the citrus seeds against fungi as long as period storage, the kind of fungi and its percentage of infection, and the citrus seeds viability. This research was carried out in September until November in 2004 at Plant Pest and Disease Laboratory and Greenhouse of Agricultural Faculty of Sebelas Maret University. The citrus seed used was Keprok variety from Ponorogo. The research design was a Randomized Completely Block Design (RCBD), with the treatments: (1) included the seeds without being stored and without being given by K. galanga extract, (2) the seeds were stored for 2 weeks with 100%, 75%, and 50% K. galanga extract, (3) the seeds were stored for 4 weeks with 100%, 75%, and 50% K. galanga extract, (4) the seeds were stored for 6 weeks with 100%, 75%, and 50% K. galanga extract, and (5) the seeds were stored for 8 weeks with 100%, 75%, and 50% K. galanga extract. Data were analyzed by using F test with 5% and 1% significance levels. If there were real differences, it was continued to be tested by using a Duncan multiple range test with 5% significance level. The results of this research showed that K. galanga extract by 50% was the best concentration that protected the citrus seeds against fungi for 2 weeks of storage. The storage of citrus seeds with K. galanga extract more than 2 weeks decreased seeds viability. The dominant kinds of fungi that attack citrus seeds in the storage were Aspergillus spp. and Penicillium spp., obtained by using a blotter test.

scholarly journals Changes in Proline Levels during Seed Development of Orthodox and Recalcitrant Seeds of Genus Acer in a Climate Change Scenario

Forests ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1362
Author(s):  
Joanna Kijowska-Oberc ◽  
Aleksandra M. Staszak ◽  
Mikołaj K. Wawrzyniak ◽  
Ewelina Ratajczak
Keyword(s):  
Climate Change ◽  
Seed Development ◽  
Desiccation Tolerance ◽  
Seed Viability ◽  
Climatic Conditions ◽  
Proline Content ◽  
Change Scenario ◽  
Acer Pseudoplatanus ◽  
Recalcitrant Seeds ◽  
Seed Desiccation

In the present study, we examined the utility of proline usage as a biochemical indicator of metabolic changes caused by climate change (mean temperature and precipitation) during seed development of two Acer species differing in desiccation tolerance: Norway maple (Acer platanoides L.—desiccation tolerant—orthodox) and sycamore (Acer pseudoplatanus L.—desiccation sensitive—recalcitrant). In plants, proline is an element of the antioxidant system, which has a role in response to water loss and high temperatures. Our study considered whether proline could be treated as an indicator of tree seed viability, crucial for genetic resources conservation. Proline content was measured biweekly in developing seeds (between 11 and 23 weeks after flowering) collected in consecutive years (2017, 2018, and 2019). We showed that proline concentrations in recalcitrant seeds were positively correlated with mean two-week temperature. In contrast, in orthodox seeds no such relationship was found. Proline content proved to be sensitive to thermal-moisture conditions changes, which makes it a promising biochemical marker of seed desiccation tolerance in different climatic conditions.

scholarly journals Die or survive? Redox changes as seed viability markers

2015 ◽  
Vol 38 (6) ◽  
pp. 1008-1010 ◽  
Author(s):  
GÁBOR KOCSY
Keyword(s):  
Seed Viability ◽  
Viability Markers

scholarly journals TEKNIK PENGEMASAN BENIH KAKAO (Theobroma cacao L) DALAM PENYIMPANAN

2018 ◽  
Vol 3 (2) ◽  
pp. 89
Author(s):  
Yulistiati Nengsih
Keyword(s):  
Low Temperature ◽  
Moisture Content ◽  
Water Content ◽  
Theobroma Cacao ◽  
Germination Rate ◽  
Seed Viability ◽  
Seed Storage ◽  
Recalcitrant Seeds ◽  
Fusarium Spp ◽  
Theobroma Cacao L

Cocoa seeds are included in recalcitrant seeds that have the characteristic of aging and regrowth of seeds during storage, viability of seeds decreases when their water content is decreased (12-31%) and can not stand to be stored at low temperature and humidity. For the provision of quality seeds need to be mastered technology seed storage properly. The aim of this research is to get the proper packing technique in maintaining viability of cocoa seed (Theobroma cacao L) during storage. The research will be conducted from Pebruari to Juni 2018, at Batanghari University Basic Laboratory. The design used is the Randomized Complete environment with the treatment of cardboard packaging with several ventilation holes: P0 = cardboard without ventilation, P1 = 2% ventilation box, P2 = 4% ventilation box, P3 = 6% ventilation box and P4 = cardboard box 8 %. The observed variables were: percentage of moisture content, percentage of seeds germinated in storage, percentage of sprout gain after storage, germination rate after storage, percentage of moldy seeds in storage and identification mushrooms. To see the effect, F test was done at 5% level, then continued with Duncan test at ? 5%. The result of the research showed that the treatment of the packing ventilation had an effect on the percentage parameter of moldy and germinated seed in storage and seed viability and vigor. In the treatment between ventilation, did not show any difference, but economically recommended the use of ventilation by 8%. Until storage on day 12 of the treatment of packaging vents can withstand the fungus attack 17.19% compared to control. The amount of ventilation does not show ability to withstand viability and vigor after storage. The fungus identified in seed storage is Fusarium spp. and Aspergillus sppKeywords: Recalcitrant, viability, moisture content of seedsAbstrakBenih kakao termasuk benih rekalsitran yang mempunyai sifat mengalami penuaan dan kemunduran benih selama penyimpanan, viabilitas benih menurun apabila diturunkan kadar airnya (12-31%) dan tidak tahan disimpan pada suhu dan kelembaban rendah. Untuk pengadaan benih berkualitas perlu dikuasai teknologi penyimpanan benih secara tepat. Penelitian bertujuan untuk mendapatkan teknik pengemasan yang tepat dalam mempertahankan viabilitas benih kakao (Theobroma cacao. L) selama penyimpanan. Penelitian dilaksanakan pada bulan Pebruari sampai Juni 2018, di Laboratorium Dasar Universitas Batanghari. Rancangan yang digunakan adalah rancangan lingkungan Acak Lengkap dengan perlakuan kemasan kardus dengan beberapa lubang ventilasi yaitu : P0= kardus tanpa ventilasi, P1= kardus ventilasi 2%, P2= kardus ventilasi 4%, P3= kardus ventilasi 6 % dan P4= kardus ventilasi 8%. Peubah yang diamati adalah: Kadar air benih, persentase benih yang berkecambah dalam penyimpanan, persentase daya kecambah setelah penyimpanan, kecepatan berkecambah setelah penyimpanan, persentase benih berjamur dalam penyimpanan dan identifikasi jamur. Untuk melihat pengaruh perlakuan, dilakukan uji F pada taraf 5%, kemudian dilanjutkan dengan uji Duncan pada taraf ? 5%. Hasil penelitian menunjukkan bahwa perlakuan ventilasi pengemasan berpengaruh terhadap peubah persentase benih berjamur dan berkecambah dalam penyimpanan serta viabilitas dan vigor benih. Pada perlakuan antar ventilasi, tidak menunjukkan adanya perbedaan, akan tetapi secara ekonomis dianjurkan penggunaan ventilasi sebesar 8%. Sampai penyimpanan pada hari ke-12 perlakuan ventilasi kemasan dapat menahan serangan jamur 17,19% dibandingkan kontrol. Jumlah ventilasi tidak menunjukkan mampu menahan viabilitas dan vigor setelah penyimpanan. Jamur yang teridentifikasi dalam penyimpanan benih adalah Fusarium spp. dan Aspergillus spp.Kata kunci : Rekalsitran, viabilitas, kadar air benih

Structural aspects and trends in the evolution of recalcitrant seeds in dicotyledons

1994 ◽  
Vol 4 (2) ◽  
pp. 225-239 ◽  
Author(s):  
I. von Teichman ◽  
A. E. van Wyk
Keyword(s):  
Seed Viability ◽  
Endosperm Development ◽  
Functional Adaptation ◽  
Evolutionary Status ◽  
Character State ◽  
Recalcitrant Seeds ◽  
Recalcitrant Seed ◽  
Ancestral Character State ◽  
Storage Reserve ◽  
Orthodox Seeds

AbstractThis review focuses on the possible evolutionary status and functional significance of recalcitrance and certain associated ovule/seed characters, within the framework of modern systems of angiosperm classification. The presence of recalcitrant seed viability in 45 dicotyledonous families is significantly associated with bitegmic and crassinucellate ovules and with nuclear endosperm development, all considered ancestral (plesiomorphic) character states of the ovule; as well as with greater seed size, woody habit and tropical habitat, also regarded as ancestral character states in the dicotyledons. In many species with recalcitrant seeds, the predominant storage reserve is carbohydrate. Recalcitrance is significantly associated with the exalbuminous type of reserve storage. It is proposed that in large recalcitrant seeds, the transfer of the main storage function from endosperm to embryo was probably an early development. In many species with recalcitrance, the ovules/seeds are characterized by extensive vascularization of the integument(s)/seed coat or by a pachychalaza. Pachychalazy is proposed to be a significant functional adaptation for a more efficient transfer of nutrients to the embryo/seed. Recalcitrance and some of the other character states proposed to be plesiomorphic in dicotyledons are also present in some gymnosperms, including presumed sister groups of the dicotyledons. In relatively advanced dicotyledonous families, mostly with orthodox seeds, recalcitrance probably persisted only in isolated relict members. Determination of character polarity is particularly problematic at lower taxonomic levels, because there is always the possibility that, in some taxa, a character state such as recalcitrance may have arisen secondarily as a reversal. Available evidence supports our view that seed recalcitrance can be regarded as a relatively ancestral character state in dicotyledons.

‘Sub-imbibed’ storage is not an option for extending longevity of recalcitrant seeds of the tropical species, Trichilia dregeana Sond.

2000 ◽  
Vol 10 (3) ◽  
pp. 355-363 ◽  
Author(s):  
Penelope J. Drew ◽  
N.W. Pammenter ◽  
Patricia Berjak
Keyword(s):  
Water Content ◽  
Seed Viability ◽  
Indirect Evidence ◽  
Storage Period ◽  
Dry Mass ◽  
Tropical Species ◽  
Recalcitrant Seeds ◽  
Initial Exposure ◽  
Short Period ◽  
Extensive Cell

AbstractRecalcitrant seeds of Trichilia dregeana were stored at 16 or 25°C, either at the water content at which they were shed or partially dried. Although having been exposed to a short period (approx. 6 h) at temperatures up to 30°C prior to storage, seeds at the original water content maintained viability for several weeks at 16°C. However, storage of undried seeds at 25°C was deleterious within 8 d, indicating a chemical basis for degeneration of hydrated recalcitrant seeds. Seeds that had been mildly dehydrated to the relatively high axis level of 1.68 g H2O g—1 dry mass, while maintaining full germinability immediately after drying, exhibited only 4% viability after 8 d in storage at 16°C and had completely lost viability after the same storage period at 25°C. Ultrastructural features characterizing hydrated seeds included indications of enhanced activity associated with initial exposure to the elevated temperature as well as some signs of stress. However, over an effective 15 d storage period at 16°C, ultrastructural features showed the cells to have retained little damage and to have been in an enhanced state of activity commensurate with ongoing development towards germination. After a longer storage period, however, signs of damage, including indirect evidence for disarray of the cytoskeleton in some axis cells, became apparent in line with the declining seed viability. Immediately following dehydration from an average axis water content of 1.97 to 1.68 H2O g g—1(the sub-imbibed condition), some ultrastructural abnormalities were apparent, but the seeds remained 100% germinable. However, within the 8 d storage period in this sub-imbibed condition, a spectrum of severe ultrastructural degeneration, including indirect evidence of the collapse of the nucleoskeleton and extensive cell lysis, accompanied viability decline of the seeds to 4%.

Methods for rapid determination of pecan (Carya illinoensis) seed viability

2017 ◽  
Vol 45 (2) ◽  
pp. 455-464
Author(s):  
T.T. Xue ◽  
J. Liu ◽  
Y.B. Shen ◽  
G.Q. Liu
Keyword(s):  
Rapid Determination ◽  
Seed Viability ◽  
Carya Illinoensis

Effect of Electromagnetic Radiation and Temperature on Wheat Seed Viability

2002 ◽  
Vol 57 (2-3) ◽  
pp. 7
Author(s):  
N. I. Grigor'eva ◽  
L. M. Chepel ◽  
S. P. Sirenko ◽  
T. M. Cheshko ◽  
V. I. Kiyko
Keyword(s):  
Electromagnetic Radiation ◽  
Seed Viability ◽  
Wheat Seed
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