Mutation of the riceASL2 gene encoding plastid ribosomal protein L21 causes chloroplast developmental defects and seedling death

Plant Biology ◽  
2014 ◽  
Vol 17 (3) ◽  
pp. 599-607 ◽  
Author(s):  
D. Lin ◽  
Q. Jiang ◽  
K. Zheng ◽  
S. Chen ◽  
H. Zhou ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Gene Encoding ◽  
Developmental Defects ◽  
Plastid Ribosomal Protein

scholarly journals Disruption of the Rice Plastid Ribosomal Protein S20 Leads to Chloroplast Developmental Defects and Seedling Lethality

2013 ◽  
Vol 3 (10) ◽  
pp. 1769-1777 ◽  
Author(s):  
Xiaodi Gong ◽  
Quan Jiang ◽  
Jianlong Xu ◽  
Jianhui Zhang ◽  
Sheng Teng ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Developmental Defects ◽  
Plastid Ribosomal Protein ◽  
Seedling Lethality

scholarly journals Identification of a gene encoding the predicted ribosomal protein L7b divergently transcribed fromPOL1in fission yeastSchizosaccharomyces pombe

1991 ◽  
Vol 19 (5) ◽  
pp. 1099-1104 ◽  
Author(s):  
Véronigue Damagnez ◽  
Anne-Marie de Recondo ◽  
Guiseppe Baldacci
Keyword(s):  
Ribosomal Protein ◽  
Gene Encoding

Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits

1993 ◽  
Vol 13 (5) ◽  
pp. 2835-2845
Author(s):  
M Deshmukh ◽  
Y F Tsay ◽  
A G Paulovich ◽  
J L Woolford
Keyword(s):  
Ribosomal Protein ◽  
Ribosomal Subunit ◽  
Single Copy ◽  
5S Rrna ◽  
Gene Encoding ◽  
Ribosomal Subunits ◽  
Ribosomal Protein L1 ◽  
The Stability ◽  
And Function

Ribosomal protein L1 from Saccharomyces cerevisiae binds 5S rRNA and can be released from intact 60S ribosomal subunits as an L1-5S ribonucleoprotein (RNP) particle. To understand the nature of the interaction between L1 and 5S rRNA and to assess the role of L1 in ribosome assembly and function, we cloned the RPL1 gene encoding L1. We have shown that RPL1 is an essential single-copy gene. A conditional null mutant in which the only copy of RPL1 is under control of the repressible GAL1 promoter was constructed. Depletion of L1 causes instability of newly synthesized 5S rRNA in vivo. Cells depleted of L1 no longer assemble 60S ribosomal subunits, indicating that L1 is required for assembly of stable 60S ribosomal subunits but not 40S ribosomal subunits. An L1-5S RNP particle not associated with ribosomal particles was detected by coimmunoprecipitation of L1 and 5S rRNA. This pool of L1-5S RNP remained stable even upon cessation of 60S ribosomal subunit assembly by depletion of another ribosomal protein, L16. Preliminary results suggest that transcription of RPL1 is not autogenously regulated by L1.

The gene encoding ribosomal protein S19 is mutated in Diamond-Blackfan anaemia

10.1038/5951 ◽  
1999 ◽  
Vol 21 (2) ◽  
pp. 169-175 ◽  
Author(s):  
Natalia Draptchinskaia ◽  
Peter Gustavsson ◽  
Björn Andersson ◽  
Monica Pettersson ◽  
Thiébaut-Noël Willig ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Gene Encoding ◽  
Ribosomal Protein S19 ◽  
Diamond Blackfan Anaemia

scholarly journals In Vitro Selection of Resistance in Haemophilus influenzae by Amoxicillin-Clavulanate, Cefpodoxime, Cefprozil, Azithromycin, and Clarithromycin

2002 ◽  
Vol 46 (9) ◽  
pp. 2956-2962 ◽  
Author(s):  
Catherine Clark ◽  
Bülent Bozdogan ◽  
Mihaela Peric ◽  
Bonifacio Dewasse ◽  
Michael R. Jacobs ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
In Vitro Selection ◽  
Fold Increase ◽  
Single Step ◽  
23S Rrna ◽  
Gene Encoding ◽  
Amoxicillin Clavulanate

ABSTRACT Abilities of amoxicillin-clavulanate, cefpodoxime, cefprozil, azithromycin, and clarithromycin to select resistant mutants of Haemophilus influenzae were tested by multistep and single-step methodologies. For multistep studies, 10 random strains were tested: 5 of these were β-lactamase positive. After 50 daily subcultures in amoxicillin-clavulanate, MICs did not increase more than fourfold. However, cefprozil MICs increased eightfold for one strain. Clarithromycin and azithromycin gave a >4-fold increase in 8 and 10 strains after 14 to 46 and 20 to 50 days, respectively. Mutants selected by clarithromycin and azithromycin were associated with mutations in 23S rRNA and ribosomal proteins L4 and L22. Three mutants selected by clarithromycin or azithromycin had alterations in ribosomal protein L4, while five had alterations in ribosomal protein L22. Two mutants selected by azithromycin had mutations in the gene encoding 23S rRNA: one at position 2058 and the other at position 2059 (Escherichia coli numbering), with replacement of A by G. One clone selected by clarithromycin became hypersusceptible to macrolides. In single-step studies azithromycin and clarithromycin had the highest mutation rates, while amoxicillin-clavulanate had the lowest. All resistant clones were identical to parents as observed by pulsed-field gel electrophoresis. The MICs of azithromycin for azithromycin-resistant clones were 16 to >128 μg/ml, and those of clarithromycin for clarithromycin-resistant clones were 32 to >128 μg/ml in multistep studies. For strains selected by azithromycin, the MICs of clarithromycin were high and vice versa. After 50 daily subcultures in the presence of drugs, MICs of amoxicillin-clavulanate and cefpodoxime against H. influenzae did not rise more than fourfold, in contrast to cefprozil, azithromycin, and clarithromycin, whose MICs rose to variable degrees.

scholarly journals Targeted Disruption of the Ribosomal Protein S19 Gene Is Lethal Prior to Implantation

2004 ◽  
Vol 24 (9) ◽  
pp. 4032-4037 ◽  
Author(s):  
Hans Matsson ◽  
Edward J. Davey ◽  
Natalia Draptchinskaia ◽  
Isao Hamaguchi ◽  
Andreas Ooka ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Normal Growth ◽  
Lethal Effect ◽  
Blastocyst Stage ◽  
Targeted Disruption ◽  
Gene Encoding ◽  
Diamond Blackfan Anemia ◽  
Associated Malformations ◽  
Ribosomal Protein S19

ABSTRACT The ribosomal protein S19 (RPS19) is located in the small (40S) subunit and is one of 79 ribosomal proteins. The gene encoding RPS19 is mutated in approximately 25% of patients with Diamond-Blackfan anemia, which is a rare congenital erythroblastopenia. Affected individuals present with decreased numbers or the absence of erythroid precursors in the bone marrow, and associated malformations of various organs are common. We produced C57BL/6J mice with a targeted disruption of murine Rps19 to study its role in erythropoiesis and development. Mice homozygous for the disrupted Rps19 were not identified as early as the blastocyst stage, indicating a lethal effect. In contrast, mice heterozygous for the disrupted Rps19 allele have normal growth and organ development, including that of the hematopoietic system. Our findings indicate that zygotes which are Rps19 −/− do not form blastocysts, whereas one normal Rps19 allele in C57BL/6J mice is sufficient to maintain normal ribosomal and possibly extraribosomal functions.

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