Comparative characterization of sun exposed and sun protected skin‐derived mesenchymal‐like stem cells in variegate porphyria and healthy individuals

2020 ◽  
Author(s):  
Rémi Safi ◽  
Elie Malek ◽  
Georges Nemer ◽  
Reem Sayed ◽  
Edward Eid ◽  
...  
Keyword(s):  
Stem Cells ◽  
Healthy Individuals ◽  
Variegate Porphyria ◽  
Comparative Characterization

scholarly journals Comparative characterization of human induced pluripotent stem cells (hiPSC) derived from patients with schizophrenia and autism

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Lena-Marie Grunwald ◽  
Ricarda Stock ◽  
Kathrina Haag ◽  
Sandra Buckenmaier ◽  
Mark-Christian Eberle ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Comparative Characterization ◽  
Induced Pluripotent

scholarly journals ID3013 Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements

2017 ◽  
Vol 4 (S) ◽  
pp. 25
Author(s):  
Karuppiah Thilakavathy
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Culture Medium ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Differentiation Potential ◽  
Comparative Characterization ◽  
Medium Formulation

Preclinical studies on mesenchymal stem cells (MSC) have allowed the cells to be considered as a promising candidate for cellular therapy. The mouse is the most widely used species for studying the characteristics of MSC. In recent years, conflicting data were reported regarding growth kinetics, surface marker profile, differentiation capacity, genetic instability or malignant transformation and so forth, that may be a result of a range of factors. One of the factors probably is the culture medium formulation.  Here we have made a comparative characterization of bone marrow-derived mesenchymal stem cells (mBM-MSC), under the same experimental conditions, cultured using two common supplements, fetal bovine serum (FBS) and MesenCultTM Stimulatory Supplement (MSS). mBM-MSC isolated from the tibias of C57BL/6 mice were cultured and expanded in Dulbecco’s Modified Eagle’s Medium supplemented with either 15% FBS or 15% MSS.  Clonogenic potential, population doubling time, immunophenotyping, differentiation immunosuppression potentials and chromosome analysis of early and late passage of mBM-MSC were assessed.      The findings showed that the immunophenotype and differentiation potential of mBM-MSC were similar when cultured using these supplements irrespective of passages.  Variations were seen in clonogenic, growth, proliferation rate and immunosuppression potential of the mBM-MSC.  This study also revealed that prolonged culture will disrupt their genetic stability regardless of the supplements used.  The genetically mutated mBM-MSC were also found to maintain their stemness characteristics and immunosuppression potential.       In conclusion, culture medium formulation causes variations in the cultured MSC and may influence downstream investigation findings.

Comparative characterization of mesenchymal stem cells from different age groups of cynomolgus monkeys

2010 ◽  
Vol 53 (5) ◽  
pp. 563-572 ◽  
Author(s):  
ZhenHua Ren ◽  
JiaYin Wang ◽  
ChunLin Zou ◽  
YunQian Guan ◽  
Y. Alex Zhang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Age Groups ◽  
Cynomolgus Monkeys ◽  
Comparative Characterization
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