Comparative Characterization of Porcine Mesenchymal Stem Cells Derived from Bone Marrow Extract and Skin Tissues

2010 ◽  
Vol 16 (6) ◽  
pp. 1481-1491 ◽  
Author(s):  
Sun-A Ock ◽  
Byeong-Gyun Jeon ◽  
Gyu-Jin Rho
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Comparative Characterization

scholarly journals ID3013 Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements

2017 ◽  
Vol 4 (S) ◽  
pp. 25
Author(s):  
Karuppiah Thilakavathy
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Culture Medium ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Differentiation Potential ◽  
Comparative Characterization ◽  
Medium Formulation

Preclinical studies on mesenchymal stem cells (MSC) have allowed the cells to be considered as a promising candidate for cellular therapy. The mouse is the most widely used species for studying the characteristics of MSC. In recent years, conflicting data were reported regarding growth kinetics, surface marker profile, differentiation capacity, genetic instability or malignant transformation and so forth, that may be a result of a range of factors. One of the factors probably is the culture medium formulation.  Here we have made a comparative characterization of bone marrow-derived mesenchymal stem cells (mBM-MSC), under the same experimental conditions, cultured using two common supplements, fetal bovine serum (FBS) and MesenCultTM Stimulatory Supplement (MSS). mBM-MSC isolated from the tibias of C57BL/6 mice were cultured and expanded in Dulbecco’s Modified Eagle’s Medium supplemented with either 15% FBS or 15% MSS.  Clonogenic potential, population doubling time, immunophenotyping, differentiation immunosuppression potentials and chromosome analysis of early and late passage of mBM-MSC were assessed.      The findings showed that the immunophenotype and differentiation potential of mBM-MSC were similar when cultured using these supplements irrespective of passages.  Variations were seen in clonogenic, growth, proliferation rate and immunosuppression potential of the mBM-MSC.  This study also revealed that prolonged culture will disrupt their genetic stability regardless of the supplements used.  The genetically mutated mBM-MSC were also found to maintain their stemness characteristics and immunosuppression potential.       In conclusion, culture medium formulation causes variations in the cultured MSC and may influence downstream investigation findings.

scholarly journals Isolation and characterization of bone marrow-derived mesenchymal stem cells in Xenopus laevis

2021 ◽  
pp. 102341
Author(s):  
Rina Otsuka-Yamaguchi ◽  
Masaaki Kitada ◽  
Yasumasa Kuroda ◽  
Yoshihiro Kushida ◽  
Shohei Wakao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Xenopus Laevis ◽  
Isolation And Characterization

Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers

2009 ◽  
Vol 132 (5) ◽  
pp. 533-546 ◽  
Author(s):  
Erdal Karaoz ◽  
Ayça Aksoy ◽  
Selda Ayhan ◽  
Ayla Eker Sarıboyacı ◽  
Figen Kaymaz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Differentiation Potential ◽  
Rat Bone

Development and characterization of Gal KO porcine bone marrow‐derived mesenchymal stem cells

2021 ◽  
Author(s):  
Takeshi Kikuchi ◽  
Masuhiro Nishimura ◽  
Maki Hirata ◽  
Fuminori Tanihara ◽  
Natsuki Komori ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells

scholarly journals Isolation and characterization of turkey bone marrow‐derived mesenchymal stem cells

2019 ◽  
Vol 37 (6) ◽  
pp. 1419-1428
Author(s):  
Qian Liu ◽  
Yaxi Zhu ◽  
Jun Qi ◽  
Peter C. Amadio ◽  
Steven L. Moran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Isolation And Characterization
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