Topical application of Nexrutine inhibits ultraviolet B-induced cutaneous inflammatory responses in SKH-1 hairless mouse

2017 ◽  
Vol 34 (1) ◽  
pp. 82-90 ◽  
Author(s):  
Shamshad Alam ◽  
Anu Pal ◽  
Dhirendra Singh ◽  
Kausar Mahmood Ansari
Keyword(s):  
Topical Application ◽  
Inflammatory Responses ◽  
Hairless Mouse ◽  
Ultraviolet B

scholarly journals Anti-Wrinkle Mechanism of Melatonin in UVB Treated HaCaT Keratinocytes and Hairless Mouse via Inhibition of ROS and Sonic Hedgehog Mediated Inflammatory Proteins

2018 ◽  
Author(s):  
Eun Kyung Park ◽  
Hyo-Jung Lee ◽  
Hyemin Lee ◽  
Ju-Ha Kim ◽  
Jisung Hwang ◽  
...  
Keyword(s):  
Sonic Hedgehog ◽  
Hedgehog Signaling ◽  
Inflammatory Responses ◽  
Hairless Mouse ◽  
Ultraviolet B ◽  
Hacat Keratinocytes ◽  
Hairless Mice ◽  
Dermal Collagen ◽  
Inflammatory Proteins ◽  
Cox 2

Though melatonin is known to improve ultraviolet B (UVB)-induced oxidative damage and inflammatory conditions via blockade of nuclear factor (NF)-κB, interleukin (IL)-6, there is no report on anti-wrinkle effect of melatonin to date. Hence in the present study, anti-wrinkle mechanism of melatonin was elucidated in UVB treated HaCaT keratinocytes and hairless mice. Herein melatonin protected against a radical initiator tert-Butyl hydroperoxide (t-BOOH) induced reactive oxygen species (ROS) production, matrix metalloprotease 1 (MMP-1) and cytotoxicity in HaCaT keratinocytes. Also, melatonin suppressed the expression of sonic hedgehog (SHH) and GLI for hedgehog signaling, p-NF-kB, cyclooxygenase (COX-2), p-ERK for inflammatory responses in UVB treated HaCaT keratinocytes. Furthermore, melatonin protected skin from wrinkle formation, transdermal water loss in hairless mice irradiated by UVB for 8 weeks. Notably, melatonin prevented against epidermal thickness and dermal collagen degradation in UVB irradiated hairless mice by Hematoxylin & Eosin and Masson’s trichrome staining. Taken together, these findings suggest that melatonin reduces wrinkle formation via inhibition of ROS/SHH and inflammatory proteins such as NF-kB/COX-2/ERK/MMP1.

Topical application of solubilized Reseda luteola extract inhibits ultraviolet B-induced inflammation in human volunteers in vivo

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
F Casetti ◽  
W Jung ◽  
U Wölfle ◽  
J Reuter ◽  
K Neumann ◽  
...  
Keyword(s):  
Topical Application ◽  
Ultraviolet B ◽  
Human Volunteers

Oral Administration of Lactobacillus plantarum HY7714 Protects Hairless Mouse Against Ultraviolet B-Induced Photoaging

2014 ◽  
Vol 24 (11) ◽  
pp. 1583-1591 ◽  
Author(s):  
Hyun Mee Kim ◽  
Dong Eun Lee ◽  
Soo Dong Park ◽  
Yong-Tae Kim ◽  
Yu Jin Kim ◽  
...  
Keyword(s):  
Oral Administration ◽  
Lactobacillus Plantarum ◽  
Hairless Mouse ◽  
Ultraviolet B

Protective Effect of Sanguinarine on Ultraviolet B-mediated Damages in SKH-1 Hairless Mouse Skin: Implications for Prevention of Skin Cancer

2007 ◽  
Vol 83 (4) ◽  
pp. 986-993 ◽  
Author(s):  
Haseeb Ahsan ◽  
Shannon Reagan-Shaw ◽  
David M. Eggert ◽  
Thomas C. Tan ◽  
Farrukh Afaq ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Protective Effect ◽  
Mouse Skin ◽  
Hairless Mouse ◽  
Ultraviolet B ◽  
Hairless Mouse Skin

A vitamin D analogue KH 1060 activates the protein kinase C– c-fos signalling pathway to stimulate epidermal proliferation in murine skin

1994 ◽  
Vol 143 (3) ◽  
pp. 521-525 ◽  
Author(s):  
R Gniadecki
Keyword(s):  
Vitamin D ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Topical Application ◽  
Messenger Rna ◽  
Hairless Mouse ◽  
Kinase C ◽  
Protein Kinase C Activity ◽  
Epidermal Growth

Abstract The cellular signalling pathways of a potent 20-epi-22-oxa vitamin D3 analogue (KH 1060) were examined in vivo in a hairless mouse model. Seventy two hours after a single topical application of KH 1060 a thickening of the epidermis (from 24·8 ±1·2 μm at 0·01 pmol/cm2 KH 1060 to 124·2 ± 6 μm at 5 pmol/cm2 KH 1060, P<0·001) was elicited due to epidermal hyperproliferation. This effect could be blocked by topical 2·5 μmol/cm sphingosine, an inhibitor of protein kinase C. Two hours after topical application of 2·5 pmol/cm2 KH 1060 a translocation of protein kinase C activity from cytoplasm to the membrane fractions was observed. Moreover, using a reverse-transcription polymerase chain reaction technique, a transient upregulation of c-fos gene expression was seen 2 hours after topical treatment with KH 1060. The expression of c-fos was dependent on protein kinase C activation, since after pretreatment with the protein kinase C blocker sphingosine, c-fos messenger RNA was not detected. These findings strongly suggest that KH 1060 stimulates epidermal growth through activation of the protein kinase C - c-fos signalling axis in vivo. Journal of Endocrinology (1994) 143, 521–525

Topical application of spent coffee ground extracts protects skin from ultraviolet B-induced photoaging in hairless mice

2016 ◽  
Vol 15 (6) ◽  
pp. 779-790 ◽  
Author(s):  
Hyeon-Son Choi ◽  
Eu Ddeum Park ◽  
Yooheon Park ◽  
Sung Hee Han ◽  
Ki Bae Hong ◽  
...  
Keyword(s):  
Topical Application ◽  
Ultraviolet B ◽  
Hairless Mice ◽  
Spent Coffee Ground ◽  
Coffee Ground

The topical application of SCG-derived OSCG and ESCG effectively protected the skin against UVB-induced photoaging in mice.

scholarly journals Ultraviolet B radiation induction of ornithine decarboxylase gene expression in mouse epidermis

1990 ◽  
Vol 270 (3) ◽  
pp. 565-568 ◽  
Author(s):  
C F Rosen ◽  
D Gajic ◽  
Q Jia ◽  
D J Drucker
Keyword(s):  
Gene Expression ◽  
Ornithine Decarboxylase ◽  
Hairless Mouse ◽  
Ultraviolet B ◽  
Dependent Manner ◽  
Mouse Epidermis ◽  
Cellular Effects ◽  
Mrna Transcripts ◽  
Maximal Induction

The cellular effects of u.v. radiation have been studied by using a hairless-mouse model in vivo. U.v. B radiation (u.v.B) induced the activity of the enzyme ornithine decarboxylase (ODC) in mouse epidermis. Maximal induction was noted after radiation with 90 mJ/cm2, and increased ODC activity was first detected 2 h after u.v.B exposure. U.v.B. also induced the expression of the ODC gene in a time- and dose-dependent manner, but did not induce the levels of actin mRNA transcripts. Cycloheximide treatment did not alter basal levels of ODC mRNA transcripts and had no effect on the u.v.B induction of ODC-gene expression. The results of these experiments demonstrate that u.v.B radiation induces both the expression of the ODC gene and the activity of the enzyme, and provides a useful ‘in vivo’ paradigm for the analysis of the molecular effects of u.v.B radiation.

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