In Vitro Effect of Photodynamic Therapy with Indocyanine Green Followed by 660 Photobiomodulation Therapy on Fibroblast Viability

2021 ◽  
Author(s):  
Ardavan Etemadi ◽  
Negar Imani ◽  
Ehsan Seyed Jafari ◽  
Nasim Chiniforush
Keyword(s):  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Photobiomodulation Therapy ◽  
Vitro Effect

scholarly journals In Vitro Effect of Photodynamic Therapy with Different Lights and Combined or Uncombined with Chlorhexidine on Candida spp.

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1176
Author(s):  
Vanesa Pérez-Laguna ◽  
Yolanda Barrena-López ◽  
Yolanda Gilaberte ◽  
Antonio Rezusta
Keyword(s):  
Photodynamic Therapy ◽  
Light Emitting Diode ◽  
Antimicrobial Effect ◽  
Photodynamic Treatment ◽  
Candida Spp ◽  
Promising Alternative ◽  
Light Emitting ◽  
Colony Forming Units ◽  
Vitro Effect

Candidiasis is very common and complicated to treat in some cases due to increased resistance to antifungals. Antimicrobial photodynamic therapy (aPDT) is a promising alternative treatment. It is based on the principle that light of a specific wavelength activates a photosensitizer molecule resulting in the generation of reactive oxygen species that are able to kill pathogens. The aim here is the in vitro photoinactivation of three strains of Candida spp., Candida albicans ATCC 10231, Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258, using aPDT with different sources of irradiation and the photosensitizer methylene blue (MB), alone or in combination with chlorhexidine (CHX). Irradiation was carried out at a fluence of 18 J/cm2 with a light-emitting diode (LED) lamp emitting in red (625 nm) or a white metal halide lamp (WMH) that emits at broad-spectrum white light (420–700 nm). After the photodynamic treatment, the antimicrobial effect is evaluated by counting colony forming units (CFU). MB-aPDT produces a 6 log10 reduction in the number of CFU/100 μL of Candida spp., and the combination with CHX enhances the effect of photoinactivation (effect achieved with lower concentration of MB). Both lamps have similar efficiencies, but the WMH lamp is slightly more efficient. This work opens the doors to a possible clinical application of the combination for resistant or persistent forms of Candida infections.

In vitro effect of curcumin-mediated antimicrobial photodynamic therapy on fibroblasts: viability and cell signaling for apoptosis

2020 ◽  
Author(s):  
Giuliana Campos Chaves Lamarque ◽  
Daniela Alejandra Cusicanqui Méndez ◽  
Adriana Arruda Matos ◽  
Thiago José Dionísio ◽  
Maria Aparecida Andrade Moreira Machado ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cell Signaling ◽  
Antimicrobial Photodynamic Therapy ◽  
Vitro Effect

In vitro efficiency and mechanistic role of indocyanine green as photodynamic therapy agent for human melanoma

2009 ◽  
Vol 6 (2) ◽  
pp. 105-116 ◽  
Author(s):  
Abdel-Megid Mamoon ◽  
Amira M. Gamal–Eldeen ◽  
Meghan E. Ruppel ◽  
Randy J. Smith ◽  
Thomas Tsang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Human Melanoma

Synthesis of isoxazoline-linked chlorins and their in vitro cell viabilities

2010 ◽  
Vol 14 (10) ◽  
pp. 859-865 ◽  
Author(s):  
Jin-Jun Wang ◽  
Jia-Zhu Li ◽  
Yun-Wei Li ◽  
Judit Jakus ◽  
Young Key Shim
Keyword(s):  
Photodynamic Therapy ◽  
Cell Line ◽  
Starting Material ◽  
Nitrile Oxide ◽  
Dipolar Cycloaddition ◽  
Mouse Sarcoma ◽  
Pyropheophorbide A ◽  
Vinyl Group ◽  
Vitro Effect

A concise synthesis of isoxazoline-linked chlorins is described. This approach is carried out from methyl pyropheophorbide-a as the starting material via 1,3-dipolar cycloaddition of a vinyl group on the periphery with nitrile oxide to give regioselective products with excellent yields. This method represents an extensive and efficient entry into the functionalization of chlorins with a chlorophyll-α skeleton. Moreover, we have examined a preliminary in vitro effect of these new derivatives on mouse sarcoma S-180 cell line in photodynamic therapy.

In-vitro effect of antimicrobial photodynamic therapy with methylene blue in two different genera of dermatophyte fungi

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Teresita Spezzia-Mazzocco ◽  
Susana A. Torres-Hurtado ◽  
Julio Cesar Ramírez-San-Juan ◽  
Rubén Ramos-García
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Antimicrobial Photodynamic Therapy ◽  
Vitro Effect

scholarly journals Influence of Incubation Time on Ortho-Toluidine Blue Mediated Antimicrobial Photodynamic Therapy Directed against Selected Candida Strains—An In Vitro Study

2021 ◽  
Vol 22 (20) ◽  
pp. 10971
Author(s):  
Rafał Wiench ◽  
Joanna Nowicka ◽  
Magdalena Pajączkowska ◽  
Piotr Kuropka ◽  
Dariusz Skaba ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Toluidine Blue ◽  
Incubation Time ◽  
In Vitro Study ◽  
Yeast Cells ◽  
Antimicrobial Photodynamic Therapy ◽  
Microscopic Evaluation ◽  
Huge Impact ◽  
Vitro Effect

(1) Background and the aim: The appropriate incubation time in the antimicrobial photodynamic therapy protocol seems to have a huge impact on the efficacy of this process. This is particularly important in relation to Candida strains, due to the size of these cells and the presence of the cell wall. The aims of this study were to determine the optimal incubation time needed for the absorption of toluidine blue by cells of C. albicans, C. glabrata, C. krusei and C. parapsilosis using direct observation by optical microscopy, and to evaluate the efficacy of TBO-mediated aPDT on planktonic cells of these strains. (2) Methods: The microscopic evaluation consisted of taking a series of images at a magnification of 600× and counting the % of stained cells. The in vitro effect of TBO-mediated aPDT combined with a diode laser (635 nm, 400mW, 12 J/cm2, CW) on the viability of yeast cells with different incubation times was evaluated. (3) Results: The presence of TBO within the cytoplasm was observed in all tested Candida strains and at all microscopic evaluation times. However, the highest percentages of cells were stained at 7 and 10 min. The highest % reduction of CFU/mL after TBO-mediated aPDT against Candida was obtained for the strain C. albicans ATCC 10,231 and it was 78.55%. (4) Conclusions: TBO-mediated aPDT against Candida was effective in reducing the number of CFU/mL at all assessed incubation times. However, the most efficient period for almost all strains was 7–10 min.

The in vitro effect of Antimicrobial Photodynamic Therapy on dental microcosm biofilms from partially erupted permanent molars: A pilot study

2018 ◽  
Vol 21 ◽  
pp. 163-167 ◽  
Author(s):  
Fabiana Sodré de Oliveira ◽  
Thiago Cruvinel ◽  
Daniela Alejandra Cusicanqui Méndez ◽  
Evandro José Dionísio ◽  
Daniela Rios ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Pilot Study ◽  
Antimicrobial Photodynamic Therapy ◽  
Permanent Molars ◽  
Vitro Effect
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