scholarly journals Influence of Incubation Time on Ortho-Toluidine Blue Mediated Antimicrobial Photodynamic Therapy Directed against Selected Candida Strains—An In Vitro Study

2021 ◽  
Vol 22 (20) ◽  
pp. 10971
Author(s):  
Rafał Wiench ◽  
Joanna Nowicka ◽  
Magdalena Pajączkowska ◽  
Piotr Kuropka ◽  
Dariusz Skaba ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Toluidine Blue ◽  
Incubation Time ◽  
In Vitro Study ◽  
Yeast Cells ◽  
Antimicrobial Photodynamic Therapy ◽  
Microscopic Evaluation ◽  
Huge Impact ◽  
Vitro Effect

(1) Background and the aim: The appropriate incubation time in the antimicrobial photodynamic therapy protocol seems to have a huge impact on the efficacy of this process. This is particularly important in relation to Candida strains, due to the size of these cells and the presence of the cell wall. The aims of this study were to determine the optimal incubation time needed for the absorption of toluidine blue by cells of C. albicans, C. glabrata, C. krusei and C. parapsilosis using direct observation by optical microscopy, and to evaluate the efficacy of TBO-mediated aPDT on planktonic cells of these strains. (2) Methods: The microscopic evaluation consisted of taking a series of images at a magnification of 600× and counting the % of stained cells. The in vitro effect of TBO-mediated aPDT combined with a diode laser (635 nm, 400mW, 12 J/cm2, CW) on the viability of yeast cells with different incubation times was evaluated. (3) Results: The presence of TBO within the cytoplasm was observed in all tested Candida strains and at all microscopic evaluation times. However, the highest percentages of cells were stained at 7 and 10 min. The highest % reduction of CFU/mL after TBO-mediated aPDT against Candida was obtained for the strain C. albicans ATCC 10,231 and it was 78.55%. (4) Conclusions: TBO-mediated aPDT against Candida was effective in reducing the number of CFU/mL at all assessed incubation times. However, the most efficient period for almost all strains was 7–10 min.

scholarly journals Efficacy of Toluidine Blue—Mediated Antimicrobial Photodynamic Therapy on Candida spp. A Systematic Review

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 349
Author(s):  
Rafał Wiench ◽  
Dariusz Skaba ◽  
Jacek Matys ◽  
Kinga Grzech-Leśniak
Keyword(s):  
Photodynamic Therapy ◽  
Toluidine Blue ◽  
Randomized Clinical Trials ◽  
Oral Candidiasis ◽  
In Vitro Studies ◽  
Cochrane Central Register ◽  
Antimicrobial Photodynamic Therapy ◽  
Candida Spp ◽  
Denture Stomatitis

The effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral yeast infections was examined many times in recent years. The authors of this review tried to address the question: “Should TBO (toluidine blue ortho)-mediated aPDT be considered a possible alternative treatment for oral candidiasis?”. PubMed/Medline and the Cochrane Central Register of Controlled Trials (CEN-TRAL) databases were searched from 1997 up to the 27th of October 2020 using a combination of the following keywords: (Candida OR Candidiasis oral OR Candidosis oral OR denture stomatitis) AND (toluidine blue OR photodynamic therapy OR aPDT OR photodynamic antimicrobial chemotherapy OR PACT OR photodynamic inactivation OR PDI). Animal studies or in vitro studies involving Candida albicans (C. albicans) and/or nonalbicans stain, randomized clinical trials (RCT) involving patients with oral candidiasis or denture stomatitis published solely in English language were included. Candida elimination method in animal, in vitro studies and RCT used was TBO-mediated aPDT. Exactly 393 studies were taken into consideration. Then, after analyzing titles and abstracts of said studies, 361 were excluded. Only 32 studies ended up being selected for in-depth screening, after which 21 of them were included in this study. All studies reported the antifungal effectiveness of aPDT with TBO against C. albicans and non-albicans Candida. In studies conducted with planktonic cells, only one study showed eradication of C. albicans. All others showed partial elimination and only one of them was not statistically significant. Experiments on yeast biofilms, in all cases, showed partial, statistically significant cell growth inhibition and weight reduction (a reduction in the number of cells—mainly hyphae) and the mass of extracellular polymeric substance (EPS). In vivo aPDT mediated by TBO exhibits antifungal effects against oral Candida spp.; however, its clinical effectiveness as a potent therapeutic strategy for oral yeast infections requires further investigation.

ERYTHROSINE AS A PHOTOSENSITIZER FOR ANTIMICROBIAL PHOTODYNAMIC THERAPY WITH BLUE LIGHT-EMITTING DIODES – AN IN VITRO STUDY

2021 ◽  
pp. 102445
Author(s):  
Marcela Leticia Leal Gonçalves ◽  
Elaine Marcílio Santos ◽  
Ana Cláudia Muniz Renno ◽  
Anna Carolina Ratto Tempestini Horliana ◽  
Matheus de Almeida Cruz ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Blue Light ◽  
Light Emitting Diodes ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antimicrobial Photodynamic Therapy ◽  
Light Emitting

In vitro effect of curcumin-mediated antimicrobial photodynamic therapy on fibroblasts: viability and cell signaling for apoptosis

2020 ◽  
Author(s):  
Giuliana Campos Chaves Lamarque ◽  
Daniela Alejandra Cusicanqui Méndez ◽  
Adriana Arruda Matos ◽  
Thiago José Dionísio ◽  
Maria Aparecida Andrade Moreira Machado ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cell Signaling ◽  
Antimicrobial Photodynamic Therapy ◽  
Vitro Effect

scholarly journals The efficacy of antimicrobial photodynamic therapy and light‐activated disinfection on zirconia implants‐ In vitro study

2019 ◽  
Vol 30 (S19) ◽  
pp. 108-108
Author(s):  
Dragana Gabric ◽  
Bleron Azizi ◽  
Veranda Azizi ◽  
Marija Peeva‐Petreska ◽  
Igor Smojver ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antimicrobial Photodynamic Therapy ◽  
Zirconia Implants

scholarly journals Type I and Type II mechanisms of antimicrobial photodynamic therapy: An in vitro study on gram-negative and gram-positive bacteria

2012 ◽  
Vol 44 (6) ◽  
pp. 490-499 ◽  
Author(s):  
Liyi Huang ◽  
Yi Xuan ◽  
Yuichiro Koide ◽  
Timur Zhiyentayev ◽  
Masamitsu Tanaka ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Vitro Study ◽  
Vitro Study ◽  
Type I ◽  
Antimicrobial Photodynamic Therapy ◽  
Type Ii ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

scholarly journals Antimicrobial photodynamic therapy and light‐activated disinfection efficacy in decontaminating titanium implant surfaces‐ In vitro study

2019 ◽  
Vol 30 (S19) ◽  
pp. 131-131
Author(s):  
Bleron Azizi ◽  
Veranda Azizi ◽  
Marija Peeva‐Petreska ◽  
Marko Vuletic ◽  
Mato Susic ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Vitro Study ◽  
Titanium Implant ◽  
Vitro Study ◽  
Antimicrobial Photodynamic Therapy

In-vitro effect of antimicrobial photodynamic therapy with methylene blue in two different genera of dermatophyte fungi

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Teresita Spezzia-Mazzocco ◽  
Susana A. Torres-Hurtado ◽  
Julio Cesar Ramírez-San-Juan ◽  
Rubén Ramos-García
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Antimicrobial Photodynamic Therapy ◽  
Vitro Effect
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