Conformational Dynamics of Sensory Rhodopsin II in Nanolipoprotein and Styrene–Maleic Acid Lipid Particles

2019 ◽  
Vol 95 (5) ◽  
pp. 1195-1204 ◽  
Author(s):  
Wageiha Mosslehy ◽  
Natalia Voskoboynikova ◽  
Alexandr Colbasevici ◽  
Adrian Ricke ◽  
Daniel Klose ◽  
...  
Keyword(s):  
Maleic Acid ◽  
Conformational Dynamics ◽  
Sensory Rhodopsin ◽  
Lipid Particles

scholarly journals Lipid Dynamics in Diisobutylene-Maleic Acid (DIBMA) Lipid Particles in Presence of Sensory Rhodopsin II

2021 ◽  
Vol 22 (5) ◽  
pp. 2548
Author(s):  
Natalia Voskoboynikova ◽  
Philipp Orekhov ◽  
Marine Bozdaganyan ◽  
Felix Kodde ◽  
Malte Rademacher ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Maleic Acid ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Coarse Grained ◽  
Spin Labels ◽  
Resonance Spectroscopy ◽  
Sensory Rhodopsin ◽  
Lipid Dynamics ◽  
Lipid Ratio ◽  
Lipid Particles

Amphiphilic diisobutylene/maleic acid (DIBMA) copolymers extract lipid-encased membrane proteins from lipid bilayers in a detergent-free manner, yielding nanosized, discoidal DIBMA lipid particles (DIBMALPs). Depending on the DIBMA/lipid ratio, the size of DIBMALPs can be broadly varied which makes them suitable for the incorporation of proteins of different sizes. Here, we examine the influence of the DIBMALP sizes and the presence of protein on the dynamics of encased lipids. As shown by a set of biophysical methods, the stability of DIBMALPs remains unaffected at different DIBMA/lipid ratios. Coarse-grained molecular dynamics simulations confirm the formation of viable DIBMALPs with an overall size of up to 35 nm. Electron paramagnetic resonance spectroscopy of nitroxides located at the 5th, 12th or 16th carbon atom positions in phosphatidylcholine-based spin labels reveals that the dynamics of enclosed lipids are not altered by the DIBMALP size. The presence of the membrane protein sensory rhodopsin II from Natronomonas pharaonis (NpSRII) results in a slight increase in the lipid dynamics compared to empty DIBMALPs. The light-induced photocycle shows full functionality of DIBMALPs-embedded NpSRII and a significant effect of the protein-to-lipid ratio during preparation on the NpSRII dynamics. This study indicates a possible expansion of the applicability of the DIBMALP technology on studies of membrane protein–protein interaction and oligomerization in a constraining environment.

Membrane proteins: is the future disc shaped?

2016 ◽  
Vol 44 (4) ◽  
pp. 1011-1018 ◽  
Author(s):  
Sarah C. Lee ◽  
Naomi L. Pollock
Keyword(s):  
Membrane Proteins ◽  
Maleic Acid ◽  
Membrane Lipids ◽  
Biological Membranes ◽  
Structure And Function ◽  
Amphiphilic Copolymer ◽  
New Approach ◽  
Analysis Techniques ◽  
Lipid Particles ◽  
And Function

The use of styrene maleic acid lipid particles (SMALPs) for the purification of membrane proteins (MPs) is a rapidly developing technology. The amphiphilic copolymer of styrene and maleic acid (SMA) disrupts biological membranes and can extract membrane proteins in nanodiscs of approximately 10 nm diameter. These discs contain SMA, protein and membrane lipids. There is evidence that MPs in SMALPs retain their native structures and functions, in some cases with enhanced thermal stability. In addition, the method is compatible with biological buffers and a wide variety of biophysical and structural analysis techniques. The use of SMALPs to solubilize and stabilize MPs offers a new approach in our attempts to understand, and influence, the structure and function of MPs and biological membranes. In this review, we critically assess progress with this method, address some of the associated technical challenges, and discuss opportunities for exploiting SMA and SMALPs to expand our understanding of MP biology.

scholarly journals Diisobutylene Maleic Acid Copolymer (DIBMA) Lipid Particle: A “Stealth” Membrane Mimetic for Neutron Scattering

2020 ◽  
Author(s):  
Rong Guo ◽  
Jacob Sumner ◽  
Shuo Qian
Keyword(s):  
Membrane Proteins ◽  
Neutron Scattering ◽  
Maleic Acid ◽  
Contrast Variation ◽  
Acid Copolymer ◽  
Membrane Mimetic ◽  
Lipid Particles ◽  
Lipid Particle ◽  
Contrast Matching ◽  
Maleic Acid Copolymer

Diisobutylene maleic acid (DIBMA) has been shown to solubilize and purify membrane proteins from a native lipid bilayer into nanodiscs without the need for a detergent. To explore DIBMA lipid particles as a suitable membrane mimetic system for neutron scattering studies of membrane proteins, we measured and determined the contrast matching point of DIBMA to be ~12% (v/v) D2O—similar to that of most protiated lipid molecules, but distinct from that of regular protiated proteins, providing a natural contrast for separating neutron scattering signals. Using SANS contrast variation, we demonstrated that the scattering from the whole lipid particle can be annihilated. Further, the lipid part of the particle shows a well-defined discoidal shape with DIBMA contrast matched. These results demonstrate that the DIBMA lipid particle is an outstanding “stealth” membrane mimetic for membrane proteins.<br>

scholarly journals Membrane-active Polymers: NCMNP13-x, NCMNP21-x and NCMNP21b-x for Membrane Protein Structural Biology

2022 ◽  
Author(s):  
Thi Kim Hoang Trinh ◽  
Claudio Catalano ◽  
Youzhong Guo
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Structural Biology ◽  
Maleic Acid ◽  
Drug Targets ◽  
Design Synthesis ◽  
Native Cell ◽  
Wide Range ◽  
Lipid Particles ◽  
High Resolution Structure

Membrane proteins are a ubiquitous group of bio-macromolecules responsible for many crucial biological processes and serve as drug targets for a wide range of modern drugs. Detergent-free technologies such as styrene-maleic acid lipid particles (SMALP), diisobutylene-maleic acid lipid particles (DIBMALP), and native cell membrane nanoparticles (NCMN) systems have recently emerged as revolutionary alternatives to the traditional detergent-based approaches for membrane protein research. NCMN systems aim to create a membrane-active polymer library suitable for high-resolution structure determination. Herein, we report our design, synthesis, characterization and comparative application analyses of three novel classes of NCMN polymers, NCMNP13-x, NCMNP21-x and NCMNP21b-x. Although each NCMN polymer can solubilize various model membrane proteins and conserve native lipids into NCMN particles, only the NCMNP21b-x series reveals lipid-protein particles with good buffer compatibility and high homogeneity suitable for single-particle cryo-EM analysis. Consequently, the NCMNP21b-x polymers that bring out high-quality NCMN particles are particularly attractive for membrane protein structural biology.

scholarly journals Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene–maleic acid lipid particles

RSC Advances ◽  
2017 ◽  
Vol 7 (81) ◽  
pp. 51324-51334 ◽  
Author(s):  
N. Voskoboynikova ◽  
W. Mosslehy ◽  
A. Colbasevici ◽  
T. T. Ismagulova ◽  
D. V. Bagrov ◽  
...  
Keyword(s):  
Maleic Acid ◽  
Lipid Particles ◽  
Natronomonas Pharaonis

The archaeal receptor/transducer complex NpSRII/NpHtrII retains its integrity upon reconstitution in styrene–maleic acid lipid particles.

scholarly journals Single molecule binding of a ligand to a G-protein-coupled receptor in real time using fluorescence correlation spectroscopy, rendered possible by nano-encapsulation in styrene maleic acid lipid particles

Nanoscale ◽  
2020 ◽  
Vol 12 (21) ◽  
pp. 11518-11525 ◽  
Author(s):  
Rachael L. Grime ◽  
Joelle Goulding ◽  
Romez Uddin ◽  
Leigh A. Stoddart ◽  
Stephen J. Hill ◽  
...  
Keyword(s):  
Real Time ◽  
G Protein ◽  
Single Molecule ◽  
Maleic Acid ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
G Protein Coupled Receptor ◽  
Fluorescence Correlation ◽  
Lipid Particles ◽  
G Protein Coupled

Combining the technologies of encapsulation of GPCRs in SMA lipid particles with fluorescence correlation spectroscopy provides a versatile characterisation platform.

scholarly journals Development of Styrene Maleic Acid Lipid Particles as a Tool for Studies of Phage-Host Interactions

2020 ◽  
Vol 94 (23) ◽  
Author(s):  
Patrick A. de Jonge ◽  
Dieuwke J. C. Smit Sibinga ◽  
Oliver A. Boright ◽  
Ana Rita Costa ◽  
Franklin L. Nobrega ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Cell Surface ◽  
Host Cell ◽  
Maleic Acid ◽  
Bacterial Species ◽  
Receptor Molecule ◽  
Receptor Interactions ◽  
Lipid Particles ◽  
Host Cell Surface ◽  
Phage Receptor

ABSTRACT The infection of a bacterium by a phage starts with attachment to a receptor molecule on the host cell surface by the phage. Since receptor-phage interactions are crucial to successful infections, they are major determinants of phage host range and, by extension, of the broader effects that phages have on bacterial communities. Many receptor molecules, particularly membrane proteins, are difficult to isolate because their stability is supported by their native membrane environments. Styrene maleic acid lipid particles (SMALPs), a recent advance in membrane protein studies, are the result of membrane solubilizations by styrene maleic acid (SMA) copolymer chains. SMALPs thereby allow for isolation of membrane proteins while maintaining their native environment. Here, we explore SMALPs as a tool to isolate and study phage-receptor interactions. We show that SMALPs produced from taxonomically distant bacterial membranes allow for receptor-specific decrease of viable phage counts of several model phages that span the three largest phage families. After characterizing the effects of incubation time and SMALP concentration on the activity of three distinct phages, we present evidence that the interaction between two model phages and SMALPs is specific to bacterial species and the phage receptor molecule. These interactions additionally lead to DNA ejection by nearly all particles at high phage titers. We conclude that SMALPs are a potentially highly useful tool for phage-host interaction studies. IMPORTANCE Bacteriophages (viruses that infect bacteria or phages) impact every microbial community. All phage infections start with the binding of the viral particle to a specific receptor molecule on the host cell surface. Due to its importance in phage infections, this first step is of interest to many phage-related research and applications. However, many phage receptors are difficult to isolate. Styrene maleic acid lipid particles (SMALPs) are a recently developed approach to isolate membrane proteins in their native environment. In this study, we explore SMALPs as a tool to study phage-receptor interactions. We find that different phage species bind to SMALPs, while maintaining specificity to their receptor. We then characterize the time and concentration dependence of phage-SMALP interactions and furthermore show that they lead to genome ejection by the phage. The results presented here show that SMALPs are a useful tool for future studies of phage-receptor interactions.

Controlling Styrene Maleic Acid Lipid Particles through RAFT

2017 ◽  
Vol 18 (11) ◽  
pp. 3706-3713 ◽  
Author(s):  
Anton A. A. Smith ◽  
Henriette E. Autzen ◽  
Tomas Laursen ◽  
Vincent Wu ◽  
Max Yen ◽  
...  
Keyword(s):  
Maleic Acid ◽  
Lipid Particles
Sign in / Sign up

Export Citation Format

Share Document