scholarly journals Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene–maleic acid lipid particles

RSC Advances ◽  
2017 ◽  
Vol 7 (81) ◽  
pp. 51324-51334 ◽  
Author(s):  
N. Voskoboynikova ◽  
W. Mosslehy ◽  
A. Colbasevici ◽  
T. T. Ismagulova ◽  
D. V. Bagrov ◽  
...  

The archaeal receptor/transducer complex NpSRII/NpHtrII retains its integrity upon reconstitution in styrene–maleic acid lipid particles.

Optik ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 4259-4262 ◽  
Author(s):  
M. Lakshmipriya ◽  
R. Ezhil Vizhi ◽  
D. Rajan Babu
Keyword(s):  

2015 ◽  
Vol 26 ◽  
pp. 167-172 ◽  
Author(s):  
Samira Mandizadeh ◽  
Faezeh Soofivand ◽  
Masoud Salavati-Niasari ◽  
Samira Bagheri
Keyword(s):  

2008 ◽  
Vol 286 (14-15) ◽  
pp. 1605-1612 ◽  
Author(s):  
Jingtian Han ◽  
Patrick Silcock ◽  
A. James McQuillan ◽  
Phil Bremer

2006 ◽  
Vol 43 (12) ◽  
pp. 1945-1954 ◽  
Author(s):  
Robinson Anandakathir ◽  
Taizoon Canteenwala ◽  
Hsing‐Ling Wang ◽  
Long Y. Chiang

2006 ◽  
Vol 420 (1-2) ◽  
pp. 298-305 ◽  
Author(s):  
Y.D. Zhong ◽  
X.B. Zhao ◽  
G.S. Cao ◽  
J.P. Tu ◽  
T.J. Zhu

2016 ◽  
Vol 44 (4) ◽  
pp. 1011-1018 ◽  
Author(s):  
Sarah C. Lee ◽  
Naomi L. Pollock

The use of styrene maleic acid lipid particles (SMALPs) for the purification of membrane proteins (MPs) is a rapidly developing technology. The amphiphilic copolymer of styrene and maleic acid (SMA) disrupts biological membranes and can extract membrane proteins in nanodiscs of approximately 10 nm diameter. These discs contain SMA, protein and membrane lipids. There is evidence that MPs in SMALPs retain their native structures and functions, in some cases with enhanced thermal stability. In addition, the method is compatible with biological buffers and a wide variety of biophysical and structural analysis techniques. The use of SMALPs to solubilize and stabilize MPs offers a new approach in our attempts to understand, and influence, the structure and function of MPs and biological membranes. In this review, we critically assess progress with this method, address some of the associated technical challenges, and discuss opportunities for exploiting SMA and SMALPs to expand our understanding of MP biology.


2007 ◽  
Vol 18 (5) ◽  
pp. 605-608 ◽  
Author(s):  
Mei Na Huang ◽  
Yan Feng Luo ◽  
Jia Chen ◽  
Yong Gang Li ◽  
Chun Hua Fu ◽  
...  

Extremophiles ◽  
2008 ◽  
Vol 12 (3) ◽  
pp. 471-476 ◽  
Author(s):  
Yi Cao ◽  
Li Liao ◽  
Xue-wei Xu ◽  
Aharon Oren ◽  
Ce Wang ◽  
...  

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